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The Effects of Inflammatory Tooth Pain on Anxiety in Adult Male Rats Publisher



Raoof M1 ; Ebrahimnejad H2 ; Abbasnejad M3 ; Amirkhosravi L4 ; Raoof R4 ; Mahani SE4 ; Ramazani M5 ; Shokouhinejad N6 ; Khoshkhounejad M6
Authors
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Authors Affiliations
  1. 1. Oral and Dental Diseases Research Center, Kerman University of Medical Sciences, Kerman, Iran
  2. 2. Department of Oral and Maxillofacial Radiology, Faculty of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran
  3. 3. Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran
  4. 4. Laboratory of Molecular Neuroscience, Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
  5. 5. Department of Endodontics, Faculty of Dentistry, Mazandaran University of Medical Sciences, Mazandaran, Iran
  6. 6. Dental Research Center, Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran

Source: Basic and Clinical Neuroscience Published:2016


Abstract

Introduction: This study aimed to examine the effects of induced inflammatory tooth pain on anxiety level in adult male rats. Methods: The mandibular incisors of 56 adult male rats were cut off and prefabricated crowns were fixed on the teeth. Formalin and capsaicin were injected intradentally to induce inflammatory tooth pain. Diazepam treated group received diazepam 30 minutes before intradental injection. The anxiety-related behavior was evaluated with elevated plus maze test. Results: Intradental application of chemical noxious stimuli, capsaicin and formalin, significantly affected nociceptive behaviors (P<0.001). Capsaicin (P<0.001) and formalin (P<0.01) significantly increased the anxiety levels in rats by decrease in the duration of time spent in open arm and increase in the duration of time spent in closed arm. Rats that received capsaicin made fewer open arm entries compared to the control animals (P<0.05). Capsaicin (P<0.001) and formalin (P<0.01) treated rats showed more stretch attend postures compared to the control and sham operated animals. In diazepam-pretreated rats, capsaicin induced algesic effect was prevented (P<0.001). Conclusion: Inflammatory pulpal pain has anxiogenic effect on rats, whereas diazepam premedication showed both anxiolytic and pain reducing effects.
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