Combination of Minimal Residual Disease on Day 15 and Copy Number Alterations Results in Bcr-Abl1-Negative Pediatric B-All: A Powerful Tool for Prediction of Induction Failure

Combination of Minimal Residual Disease on Day 15 and Copy Number Alterations Results in Bcr-Abl1-Negative Pediatric B-All: A Powerful Tool for Prediction of Induction Failure Publisher Pubmed

Baghdadi H¹ ; Soleimani M¹ ; Zavvar M² ; Bahoush G³ ; Poopak B⁴

Show Affiliations

1. Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, 14115111, Iran
2. Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, 443614177, Iran
3. Department of Pediatrics, Ali-Asghar Children Hospital, Faculty of Medicine, Iran University of Medical Sciences, Tehran, 14665354, Iran
4. Islamic Azad University, Tehran Medical Sciences Branch, Tehran, 193951495, Iran

Source: Cancer Genetics Published:2024

Abstract

The current genomic abnormalities provide prognostic value in pediatric Acute Lymphoblastic Leukemia (ALL). Furthermore, Copy Number Alteration (CNA) has recently been used to improve the genetic risk stratification of patients. This study aimed to evaluate CNA profiles in BCR-ABL1-negative pediatric B-ALL patients and correlate the data with Minimal Residual Disease (MRD) results after induction therapy. We examined 82 bone marrow samples from pediatric BCR-ABL1-negative B-ALL using the MLPA method for the most common CNAs, including IKZF1, CDKN2A/B, PAX5, RB1, BTG1, ETV6, EBF1, JAK2, and PAR1 region. Subsequently, patients were followed-up by multiparameter Flow Cytometry for MRD (MFC-MRD) assessment on days 15 and 33 after induction. Data showed that 58.5 % of patients carried at least one gene deletion, whereas 41.7 % of them carried more than one gene deletion simultaneously. The most frequent gene deletions were CDKN2A/B, ETV6, and IKZF1 (30.5 %, 14.6 %, and 14.6 %, respectively), while the PAR1 region showed predominantly duplication (30.5 %). CDKN2A/B and IKZF1 were related to positive MRD results on day 15 (p = 0.003 and p = 0.007, respectively). The simultaneous presence of more than one deletion was significantly associated with high induction failure (p = 0.001). Also, according to the CNA profile criteria, the CNA with poor risk (CNA-PR) profile was statistically associated with older age and positive MRD results on day 15 (p = 0.014 and p = 0.013, respectively). According to our results, the combined use of CNAs with MRD results on day 15 can predict induction failure and be helpful in ameliorating B-ALL risk stratification and treatment approaches. © 2023

Style	Citing Format
MLA	Baghdadi H, et al.. "Combination of Minimal Residual Disease on Day 15 and Copy Number Alterations Results in Bcr-Abl1-Negative Pediatric B-All: A Powerful Tool for Prediction of Induction Failure." Cancer Genetics, vol. 282-283, no. , 2024, pp. 27-34.
APA	Baghdadi H, Soleimani M, Zavvar M, Bahoush G, Poopak B (2024). Combination of Minimal Residual Disease on Day 15 and Copy Number Alterations Results in Bcr-Abl1-Negative Pediatric B-All: A Powerful Tool for Prediction of Induction Failure. Cancer Genetics, 282-283(), 27-34.
Chicago	Baghdadi H, Soleimani M, Zavvar M, Bahoush G, Poopak B. "Combination of Minimal Residual Disease on Day 15 and Copy Number Alterations Results in Bcr-Abl1-Negative Pediatric B-All: A Powerful Tool for Prediction of Induction Failure." Cancer Genetics 282-283, no. (2024): 27-34.
Harvard	Baghdadi H et al. (2024) 'Combination of Minimal Residual Disease on Day 15 and Copy Number Alterations Results in Bcr-Abl1-Negative Pediatric B-All: A Powerful Tool for Prediction of Induction Failure', Cancer Genetics, 282-283(), pp. 27-34.
Vancouver	Baghdadi H, Soleimani M, Zavvar M, Bahoush G, Poopak B. Combination of Minimal Residual Disease on Day 15 and Copy Number Alterations Results in Bcr-Abl1-Negative Pediatric B-All: A Powerful Tool for Prediction of Induction Failure. Cancer Genetics. 2024;282-283():27-34.
BibTex	@article{ author = {Baghdadi H and Soleimani M and Zavvar M and Bahoush G and Poopak B}, title = {Combination of Minimal Residual Disease on Day 15 and Copy Number Alterations Results in Bcr-Abl1-Negative Pediatric B-All: A Powerful Tool for Prediction of Induction Failure}, journal = {Cancer Genetics}, volume = {282-283}, number = {}, pages = {27-34}, year = {2024} }
RIS	TY - JOUR AU - Baghdadi H AU - Soleimani M AU - Zavvar M AU - Bahoush G AU - Poopak B TI - Combination of Minimal Residual Disease on Day 15 and Copy Number Alterations Results in Bcr-Abl1-Negative Pediatric B-All: A Powerful Tool for Prediction of Induction Failure JO - Cancer Genetics VL - 282-283 IS - SP - 27 EP - 34 PY - 2024 ER -

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