Isolation and Identification of Specific Bacteriophage Against Enteropathogenic Escherichia Coli (Epec) and in Vitro and in Vivo Characterization of Bacteriophage

Isolation and Identification of Specific Bacteriophage Against Enteropathogenic Escherichia Coli (Epec) and in Vitro and in Vivo Characterization of Bacteriophage Publisher Pubmed

Vahedi A¹ ; Dallal MMS² ; Douraghi M³ ; Nikkhahi F⁴ ; Rajabi Z⁵ ; Yousefi M⁶ ; Mousavi M⁷

Show Affiliations

1. School of Public Health, Tehran University of Medical Sciences, Ghods street, Tehran, 3551713146, Iran
2. Department of Microbiology and Pathobiology, Tehran University of Medical Sciences, Enghelab Sq., Tehran, 1416753955, Iran
3. Department of Pathology, Tehran University of Medical Sciences, Poursina St., Tehran, 14185781, Iran
4. Department of Medical Bacteriology-Pathobiology, Tehran University of Medical Sciences, District 6 Pour Sina St, Tehran, 1416753955, Iran
5. Food Microbiology Research Center, Tehran University of Medical Sciences, Ghods st, Tehran, 1416753955, Iran
6. Department of Virology, Tehran University of Medical Sciences, Ghods street, Tehran, 1416753955, Iran
7. Department of Biostatistics, Faculty of medical sciences, Tarbiat Modares University, Tehran, 3551713146, Iran

Source: FEMS Microbiology Letters Published:2018

Abstract

In recent years, the increasing resistance of enteropathogenic Escherichia coli (EPEC) to commonly used antibiotics has made it difficult to choose the best treatment option. Bacteriophage therapy could be a potent alternative to antibiotic therapy for antibiotic-resistant bacteria. The aim of the present study was to isolate and identify a specific bacteriophage against EPEC and characterize bacteriophage in vitro and in vivo. The specific bacteriophage was isolated, and the effect of phage therapy on 48 mice (Balb/c) was investigated. Animals were divided into six groups, including A: PBS (negative control); B: bacteria (positive control); C: bacteria + ciprofloxacin (after 24 h); D: bacteria + bacteriophage (after 24 h); E: bacteria + ciprofloxacin + bacteriophage (after 24 h) and F: bacteriophage + bacteria (after 24 h). Specific bacteriophage against EPEC was isolated from hospital sewage. The bacteriophage had an icosahedral head (120 nm) and a tail (138 nm). The single dose of the bacteriophage (2 × 109 pfu ml-1) was able to control the infection. Unfortunately, because of the misuse of antibiotics by EPEC infected patients, the antibiotic resistant bacteria will become prevalent in the future and the treatment of EPEC infection is going to become more difficult than ever. © FEMS 2018.

2. Isolation and in Vitro Characterization of Specific Bacteriophages Against Methicillin-Resistant Staphylococcus Aureus, Infection# Epidemiology and Microbiology (2022)

Experts (# of related papers)

Yousef Erfani (1)

Style	Citing Format
MLA	Vahedi A, et al.. "Isolation and Identification of Specific Bacteriophage Against Enteropathogenic Escherichia Coli (Epec) and in Vitro and in Vivo Characterization of Bacteriophage." FEMS Microbiology Letters, vol. 365, no. 16, 2018, pp. -.
APA	Vahedi A, Dallal MMS, Douraghi M, Nikkhahi F, Rajabi Z, Yousefi M, Mousavi M (2018). Isolation and Identification of Specific Bacteriophage Against Enteropathogenic Escherichia Coli (Epec) and in Vitro and in Vivo Characterization of Bacteriophage. FEMS Microbiology Letters, 365(16), -.
Chicago	Vahedi A, Dallal MMS, Douraghi M, Nikkhahi F, Rajabi Z, Yousefi M, Mousavi M. "Isolation and Identification of Specific Bacteriophage Against Enteropathogenic Escherichia Coli (Epec) and in Vitro and in Vivo Characterization of Bacteriophage." FEMS Microbiology Letters 365, no. 16 (2018): -.
Harvard	Vahedi A et al. (2018) 'Isolation and Identification of Specific Bacteriophage Against Enteropathogenic Escherichia Coli (Epec) and in Vitro and in Vivo Characterization of Bacteriophage', FEMS Microbiology Letters, 365(16), pp. -.
Vancouver	Vahedi A, Dallal MMS, Douraghi M, Nikkhahi F, Rajabi Z, Yousefi M, et al.. Isolation and Identification of Specific Bacteriophage Against Enteropathogenic Escherichia Coli (Epec) and in Vitro and in Vivo Characterization of Bacteriophage. FEMS Microbiology Letters. 2018;365(16):-.
BibTex	@article{ author = {Vahedi A and Dallal MMS and Douraghi M and Nikkhahi F and Rajabi Z and Yousefi M and Mousavi M}, title = {Isolation and Identification of Specific Bacteriophage Against Enteropathogenic Escherichia Coli (Epec) and in Vitro and in Vivo Characterization of Bacteriophage}, journal = {FEMS Microbiology Letters}, volume = {365}, number = {16}, pages = {-}, year = {2018} }
RIS	TY - JOUR AU - Vahedi A AU - Dallal MMS AU - Douraghi M AU - Nikkhahi F AU - Rajabi Z AU - Yousefi M AU - Mousavi M TI - Isolation and Identification of Specific Bacteriophage Against Enteropathogenic Escherichia Coli (Epec) and in Vitro and in Vivo Characterization of Bacteriophage JO - FEMS Microbiology Letters VL - 365 IS - 16 SP - EP - PY - 2018 ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract