Isolation of Hl-60 Cancer Cells From the Human Serum Sample Using Mno2-Pei/Ni/Au/Aptamer As a Novel Nanomotor and Electrochemical Determination of Thereof by Aptamer/Gold Nanoparticles-Poly(3,4-Ethylene Dioxythiophene) Modified Gc Electrode

Isolation of Hl-60 Cancer Cells From the Human Serum Sample Using Mno2-Pei/Ni/Au/Aptamer As a Novel Nanomotor and Electrochemical Determination of Thereof by Aptamer/Gold Nanoparticles-Poly(3,4-Ethylene Dioxythiophene) Modified Gc Electrode Publisher Pubmed

Amouzadeh Tabrizi M^{1, 2} ; Shamsipur M³ ; Saber R^{2, 4} ; Sarkar S^{2, 5}

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1. Nano Drug Delivery Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
2. Research Center for Science and Technology in Medicine, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Chemistry, Razi University, Kermanshah, Iran
4. School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
5. Department of Medical Physics and Biomedical Engineering Tehran University of Medical Sciences, Tehran, Iran

Source: Biosensors and Bioelectronics Published:2018

Abstract

Herein, aptamer-modified self-propelled nanomotors were used for transportation of human promyelocytic leukemia cells (HL-60) from a human serum sample. For this purpose, the fabricated manganese oxide nanosheets-polyethyleneimine decorated with nickel/gold nanoparticles (MnO2-PEI/Ni/Au) as nanomotors were added to a vial containing thiolated aptamer KH1C12 solution as a capture aptamer to attach to the gold nanoparticles on the surface of nanomotors covalently. The aptamer-modified self-propelled nanomotors (aptamerKH1C12/nanomotors) were then separated by placing the vial in a magnetic stand. The aptamer-modified self-propelled nanomotors were rinsed three times with water to remove the non-attached aptamers. Then, the resulting aptamerKH1C12/nanomotors were applied for the on-the-fly” transporting of HL-60 cancer cell from a human serum sample. To release of the captured HL-60 cancer cells, the complementary nucleotide sequences of KH1C12 aptamer solution (releasing aptamer) that has a with capture aptamer was added to phosphate buffer solution (1 M, pH 7.4) containing HL-60/aptamerKH1C12/nanomotors. Because of the high affinity of capture aptamer to complementary nucleotide sequences of aptamerKH1C12, the HL-60 cancer cells released on the surface of aptamerKH1C12/nanomotors into the solution. The second goal of the present work was determining the concentration of HL-60 cancer cell in the human serum samples. The electrochemical impedance spectroscopy technique (EIS) was used for the determination of HL-60 cancer cell. The concentration of separated cancer cell was determined by aptamer/gold nanoparticles-poly(3,4-ethylene dioxythiophene) modified GC electrode (GC/PEDOT-Aunano/aptamer KH1C12). The proposed aptasensor exhibited a good response to the concentration of HL-60 cancer cells in the range of 2.5 × 101 to 5 × 105 cells mL-1 with a low limit of detection of 250 cells mL-1. © 2018 Elsevier B.V.

Style	Citing Format
MLA	Amouzadeh Tabrizi M, et al.. "Isolation of Hl-60 Cancer Cells From the Human Serum Sample Using Mno2-Pei/Ni/Au/Aptamer As a Novel Nanomotor and Electrochemical Determination of Thereof by Aptamer/Gold Nanoparticles-Poly(3,4-Ethylene Dioxythiophene) Modified Gc Electrode." Biosensors and Bioelectronics, vol. 110, no. , 2018, pp. 141-146.
APA	Amouzadeh Tabrizi M, Shamsipur M, Saber R, Sarkar S (2018). Isolation of Hl-60 Cancer Cells From the Human Serum Sample Using Mno2-Pei/Ni/Au/Aptamer As a Novel Nanomotor and Electrochemical Determination of Thereof by Aptamer/Gold Nanoparticles-Poly(3,4-Ethylene Dioxythiophene) Modified Gc Electrode. Biosensors and Bioelectronics, 110(), 141-146.
Chicago	Amouzadeh Tabrizi M, Shamsipur M, Saber R, Sarkar S. "Isolation of Hl-60 Cancer Cells From the Human Serum Sample Using Mno2-Pei/Ni/Au/Aptamer As a Novel Nanomotor and Electrochemical Determination of Thereof by Aptamer/Gold Nanoparticles-Poly(3,4-Ethylene Dioxythiophene) Modified Gc Electrode." Biosensors and Bioelectronics 110, no. (2018): 141-146.
Harvard	Amouzadeh Tabrizi M et al. (2018) 'Isolation of Hl-60 Cancer Cells From the Human Serum Sample Using Mno2-Pei/Ni/Au/Aptamer As a Novel Nanomotor and Electrochemical Determination of Thereof by Aptamer/Gold Nanoparticles-Poly(3,4-Ethylene Dioxythiophene) Modified Gc Electrode', Biosensors and Bioelectronics, 110(), pp. 141-146.
Vancouver	Amouzadeh Tabrizi M, Shamsipur M, Saber R, Sarkar S. Isolation of Hl-60 Cancer Cells From the Human Serum Sample Using Mno2-Pei/Ni/Au/Aptamer As a Novel Nanomotor and Electrochemical Determination of Thereof by Aptamer/Gold Nanoparticles-Poly(3,4-Ethylene Dioxythiophene) Modified Gc Electrode. Biosensors and Bioelectronics. 2018;110():141-146.
BibTex	@article{ author = {Amouzadeh Tabrizi M and Shamsipur M and Saber R and Sarkar S}, title = {Isolation of Hl-60 Cancer Cells From the Human Serum Sample Using Mno2-Pei/Ni/Au/Aptamer As a Novel Nanomotor and Electrochemical Determination of Thereof by Aptamer/Gold Nanoparticles-Poly(3,4-Ethylene Dioxythiophene) Modified Gc Electrode}, journal = {Biosensors and Bioelectronics}, volume = {110}, number = {}, pages = {141-146}, year = {2018} }
RIS	TY - JOUR AU - Amouzadeh Tabrizi M AU - Shamsipur M AU - Saber R AU - Sarkar S TI - Isolation of Hl-60 Cancer Cells From the Human Serum Sample Using Mno2-Pei/Ni/Au/Aptamer As a Novel Nanomotor and Electrochemical Determination of Thereof by Aptamer/Gold Nanoparticles-Poly(3,4-Ethylene Dioxythiophene) Modified Gc Electrode JO - Biosensors and Bioelectronics VL - 110 IS - SP - 141 EP - 146 PY - 2018 ER -

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