Intra Operative Mild Cooling of Large Tumors Reduces Their Invasive and Metastatic Functions While Increasing Their Resistance to Apoptosis

Intra Operative Mild Cooling of Large Tumors Reduces Their Invasive and Metastatic Functions While Increasing Their Resistance to Apoptosis Publisher Pubmed

Tavassoli N¹ ; Ghahremani A² ; Namakin K^{2, 3} ; Naserghandi A^{2, 3} ; Miri SR¹ ; Abdolahad M²

Show Affiliations

1. Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran
2. Nano Bio Electronic Devices Lab, Nano Electronic Center of Excellence, School of Electrical and Computer Engineering, College of Engineering, University of Tehran, Tehran, Iran
3. Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Therapeutic Hypothermia and Temperature Management Published:2023

Abstract

Cancer treatment often involves excisional surgery, but this approach may leave behind minimal residual disease, leading to tumor regrowth. Proinflammatory cytokines and their role in altering residual cancerous cells postsurgery have garnered attention. The study examines how mild intraoperative cooling affects cancer cells and their gene expression. It aims to discover strategies for reducing tumor growth after surgery. Nine cases of solid tumor were included in the study, nine samples were cooled with the Peltier-Seebeck device down to12°C, and cooled and noncooled regions of tumors were analyzed using reverse transcription-polymerase chain reaction. Key transcriptomes, including neural-cadherin, cadherins (CDH), 70-kDa Heat Shock Protein (HSP70), hypoxia-inducible factor (HIF), Y-Box-binding protein 1 (YB-1), matrix metalloproteinase 9 (MMP9), and matrix metalloproteinase 2 (MMP2), were measured to assess the impact of mild hypothermia on cancer cell metabolism and cold shock responses. Analysis of cooled and noncooled regions revealed reduced MMP2/9 levels in cooled regions in five out of seven cases, indicating potential suppression of tumor invasion and metastasis. CDH-1 expression was detected in five cases, with decreased levels observed in cooled regions in most cases, suggesting a role in tumor aggressiveness. YB-1 expression was increased in six out of eight samples, possibly correlating with local recurrence and reduced overall survival times. N-Cad expression was increased in all five samples where it was detected, indicating its potential involvement in tumor cell motility and invasion. HSPs showed a mild increase in four out of five cases following cooling, potentially contributing to tumor cell resistance to cooling-induced apoptosis. Intraoperative mild cooling resulted in the downregulation of key proteins playing a role in invasion and metastasis. However, Elevated YB-1 and N-Cad expression limits cooling's universal application. Further research is necessary to comprehend cooling-related transcriptome changes and their impact on patient outcomes. Copyright 2023, Mary Ann Liebert, Inc., publishers.

Style	Citing Format
MLA	Tavassoli N, et al.. "Intra Operative Mild Cooling of Large Tumors Reduces Their Invasive and Metastatic Functions While Increasing Their Resistance to Apoptosis." Therapeutic Hypothermia and Temperature Management, vol. , no. , 2023, pp. -.
APA	Tavassoli N, Ghahremani A, Namakin K, Naserghandi A, Miri SR, Abdolahad M (2023). Intra Operative Mild Cooling of Large Tumors Reduces Their Invasive and Metastatic Functions While Increasing Their Resistance to Apoptosis. Therapeutic Hypothermia and Temperature Management, (), -.
Chicago	Tavassoli N, Ghahremani A, Namakin K, Naserghandi A, Miri SR, Abdolahad M. "Intra Operative Mild Cooling of Large Tumors Reduces Their Invasive and Metastatic Functions While Increasing Their Resistance to Apoptosis." Therapeutic Hypothermia and Temperature Management , no. (2023): -.
Harvard	Tavassoli N et al. (2023) 'Intra Operative Mild Cooling of Large Tumors Reduces Their Invasive and Metastatic Functions While Increasing Their Resistance to Apoptosis', Therapeutic Hypothermia and Temperature Management, (), pp. -.
Vancouver	Tavassoli N, Ghahremani A, Namakin K, Naserghandi A, Miri SR, Abdolahad M. Intra Operative Mild Cooling of Large Tumors Reduces Their Invasive and Metastatic Functions While Increasing Their Resistance to Apoptosis. Therapeutic Hypothermia and Temperature Management. 2023;():-.
BibTex	@article{ author = {Tavassoli N and Ghahremani A and Namakin K and Naserghandi A and Miri SR and Abdolahad M}, title = {Intra Operative Mild Cooling of Large Tumors Reduces Their Invasive and Metastatic Functions While Increasing Their Resistance to Apoptosis}, journal = {Therapeutic Hypothermia and Temperature Management}, volume = {}, number = {}, pages = {-}, year = {2023} }
RIS	TY - JOUR AU - Tavassoli N AU - Ghahremani A AU - Namakin K AU - Naserghandi A AU - Miri SR AU - Abdolahad M TI - Intra Operative Mild Cooling of Large Tumors Reduces Their Invasive and Metastatic Functions While Increasing Their Resistance to Apoptosis JO - Therapeutic Hypothermia and Temperature Management VL - IS - SP - EP - PY - 2023 ER -

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