Development of an Expression Vector Engineering Strategy Based on Tdna Insulator Element for the Stable Expression of Vascular Endothelial Growth Factor Receptor-Fc Fusion Protein

Development of an Expression Vector Engineering Strategy Based on Tdna Insulator Element for the Stable Expression of Vascular Endothelial Growth Factor Receptor-Fc Fusion Protein Publisher Pubmed

Rahimpour A^{1, 2} ; Mosallaei M³ ; Pourghazi F⁴ ; Tabatabaee SH⁵ ; Hoseinpoor R⁶ ; Pourmaleki E^{1, 2} ; Soosanabadi M^{7, 8}

Show Affiliations

1. Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3. Personalized Medicine and Genometabolomics Research Center, Hope Generation Foundation, Tehran, Iran
4. Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran
5. Department of Life Science Engineering, Faculty of New Sciences and Technology, University of Tehran, Tehran, Iran
6. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
7. Department of Medical Genetics, Semnan University of Medical Sciences, Semnan, Iran
8. Department of Genetics, School of Medicine, Jiroft University of Medical Sciences, Jiroft, Iran

Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Published:2023

Abstract

During the past decades, tremendous advances have occurred in manufacturing recombinant therapeutic proteins in Chinese hamster ovary (CHO) cells. Nevertheless, the production of stable high-producing cell lines has remained a major obstacle in the development process of the CHO cell line. It has been shown that genomic regulatory elements can promote cell line development efficiency by improving transgenes' productivity and stability. Such elements include insulators, ubiquitous chromatin opening elements, scaffold/matrix attachment regions, and antirepressors. In addition, tDNA elements are shown to act as insulators in mammalian cells. This study examines the effect of the tDNA insulator on stable expression of a vascular endothelial growth factor receptor-Fc fusion protein. © Copyright 2023, Mary Ann Liebert, Inc., publishers 2023.

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1. The Augmenting Effects of the Tdna Insulator on Stable Expression of Monoclonal Antibody in Chinese Hamster Ovary Cells, Monoclonal Antibodies in Immunodiagnosis and Immunotherapy (2018)

Style	Citing Format
MLA	Rahimpour A, et al.. "Development of an Expression Vector Engineering Strategy Based on Tdna Insulator Element for the Stable Expression of Vascular Endothelial Growth Factor Receptor-Fc Fusion Protein." Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, vol. 42, no. 4, 2023, pp. 140-144.
APA	Rahimpour A, Mosallaei M, Pourghazi F, Tabatabaee SH, Hoseinpoor R, Pourmaleki E, Soosanabadi M (2023). Development of an Expression Vector Engineering Strategy Based on Tdna Insulator Element for the Stable Expression of Vascular Endothelial Growth Factor Receptor-Fc Fusion Protein. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 42(4), 140-144.
Chicago	Rahimpour A, Mosallaei M, Pourghazi F, Tabatabaee SH, Hoseinpoor R, Pourmaleki E, Soosanabadi M. "Development of an Expression Vector Engineering Strategy Based on Tdna Insulator Element for the Stable Expression of Vascular Endothelial Growth Factor Receptor-Fc Fusion Protein." Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 42, no. 4 (2023): 140-144.
Harvard	Rahimpour A et al. (2023) 'Development of an Expression Vector Engineering Strategy Based on Tdna Insulator Element for the Stable Expression of Vascular Endothelial Growth Factor Receptor-Fc Fusion Protein', Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 42(4), pp. 140-144.
Vancouver	Rahimpour A, Mosallaei M, Pourghazi F, Tabatabaee SH, Hoseinpoor R, Pourmaleki E, et al.. Development of an Expression Vector Engineering Strategy Based on Tdna Insulator Element for the Stable Expression of Vascular Endothelial Growth Factor Receptor-Fc Fusion Protein. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. 2023;42(4):140-144.
BibTex	@article{ author = {Rahimpour A and Mosallaei M and Pourghazi F and Tabatabaee SH and Hoseinpoor R and Pourmaleki E and Soosanabadi M}, title = {Development of an Expression Vector Engineering Strategy Based on Tdna Insulator Element for the Stable Expression of Vascular Endothelial Growth Factor Receptor-Fc Fusion Protein}, journal = {Monoclonal Antibodies in Immunodiagnosis and Immunotherapy}, volume = {42}, number = {4}, pages = {140-144}, year = {2023} }
RIS	TY - JOUR AU - Rahimpour A AU - Mosallaei M AU - Pourghazi F AU - Tabatabaee SH AU - Hoseinpoor R AU - Pourmaleki E AU - Soosanabadi M TI - Development of an Expression Vector Engineering Strategy Based on Tdna Insulator Element for the Stable Expression of Vascular Endothelial Growth Factor Receptor-Fc Fusion Protein JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy VL - 42 IS - 4 SP - 140 EP - 144 PY - 2023 ER -

Style

Citing Format

MLA

Rahimpour A, et al.. "Development of an Expression Vector Engineering Strategy Based on Tdna Insulator Element for the Stable Expression of Vascular Endothelial Growth Factor Receptor-Fc Fusion Protein." Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, vol. 42, no. 4, 2023, pp. 140-144.

APA

Rahimpour A, Mosallaei M, Pourghazi F, Tabatabaee SH, Hoseinpoor R, Pourmaleki E, Soosanabadi M (2023). Development of an Expression Vector Engineering Strategy Based on Tdna Insulator Element for the Stable Expression of Vascular Endothelial Growth Factor Receptor-Fc Fusion Protein. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 42(4), 140-144.

Chicago

Rahimpour A, Mosallaei M, Pourghazi F, Tabatabaee SH, Hoseinpoor R, Pourmaleki E, Soosanabadi M. "Development of an Expression Vector Engineering Strategy Based on Tdna Insulator Element for the Stable Expression of Vascular Endothelial Growth Factor Receptor-Fc Fusion Protein." Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 42, no. 4 (2023): 140-144.

Harvard

Rahimpour A et al. (2023) 'Development of an Expression Vector Engineering Strategy Based on Tdna Insulator Element for the Stable Expression of Vascular Endothelial Growth Factor Receptor-Fc Fusion Protein', Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 42(4), pp. 140-144.

Vancouver

Rahimpour A, Mosallaei M, Pourghazi F, Tabatabaee SH, Hoseinpoor R, Pourmaleki E, et al.. Development of an Expression Vector Engineering Strategy Based on Tdna Insulator Element for the Stable Expression of Vascular Endothelial Growth Factor Receptor-Fc Fusion Protein. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. 2023;42(4):140-144.

BibTex

@article{ author = {Rahimpour A and Mosallaei M and Pourghazi F and Tabatabaee SH and Hoseinpoor R and Pourmaleki E and Soosanabadi M}, title = {Development of an Expression Vector Engineering Strategy Based on Tdna Insulator Element for the Stable Expression of Vascular Endothelial Growth Factor Receptor-Fc Fusion Protein}, journal = {Monoclonal Antibodies in Immunodiagnosis and Immunotherapy}, volume = {42}, number = {4}, pages = {140-144}, year = {2023} }

RIS

TY - JOUR
AU - Rahimpour A
AU - Mosallaei M
AU - Pourghazi F
AU - Tabatabaee SH
AU - Hoseinpoor R
AU - Pourmaleki E
AU - Soosanabadi M
TI - Development of an Expression Vector Engineering Strategy Based on Tdna Insulator Element for the Stable Expression of Vascular Endothelial Growth Factor Receptor-Fc Fusion Protein
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
VL - 42
IS - 4
SP - 140
EP - 144
PY - 2023
ER -

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