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Step-By-Step Approach: Stereotaxic Surgery for in Vivo Extracellular Field Potential Recording at the Rat Schaffer Collateral-Ca1 Synapse Using the Elab System Publisher

Summary: A study provides a guide for precise brain electrode placement in rodents, aiding neuroscience research. #BrainResearch #Neuroscience

Azimzadeh M1 ; Mohd Azmi MAN2 ; Reisi P3 ; Cheah PS4, 5 ; Ling KH1, 5
Authors

Source: MethodsX Published:2024


Abstract

In vivo extracellular field potential recording is a commonly used technique in modern neuroscience research. The success of long-term electrophysiological recordings often depends on the quality of the implantation surgery. However, there is limited use of visually guided stereotaxic neurosurgery and the application of the eLab/ePulse electrophysiology system in rodent models. This study presents a practical and functional manual guide for surgical electrode implantation in rodent models using the eLab/ePulse electrophysiology system for recording and stimulation purposes to assess neuronal functionality and synaptic plasticity. The evaluation parameters included the input/output function (IO), paired-pulse facilitation or depression (PPF/PPD), long-term potentiation (LTP), and long-term depression (LTD). • Provides a detailed picture-guided procedure for conducting in vivo stereotaxic neurosurgery. • Specifically covers the insertion of hippocampal electrodes and the recording of evoked extracellular field potentials. © 2023 The Author(s)