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Step-By-Step Approach: Stereotaxic Surgery for in Vivo Extracellular Field Potential Recording at the Rat Schaffer Collateral-Ca1 Synapse Using the Elab System Publisher



Azimzadeh M1 ; Mohd Azmi MAN2 ; Reisi P3 ; Cheah PS4, 5 ; Ling KH1, 5
Authors
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Authors Affiliations
  1. 1. Department of Biomedical Sciences Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, UPM, Selangor, Serdang, 43400, Malaysia
  2. 2. Deputy Dean's Office (Research and Internationalization), Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, UPM Serdang, Selangor, 43400, Malaysia
  3. 3. Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Selangor, UPM Serdang, 43400, Malaysia
  5. 5. Malaysian Research Institute on Ageing (MyAgeing™), Universiti Putra Malaysia, Selangor, UPM Serdang, 43400, Malaysia

Source: MethodsX Published:2024


Abstract

In vivo extracellular field potential recording is a commonly used technique in modern neuroscience research. The success of long-term electrophysiological recordings often depends on the quality of the implantation surgery. However, there is limited use of visually guided stereotaxic neurosurgery and the application of the eLab/ePulse electrophysiology system in rodent models. This study presents a practical and functional manual guide for surgical electrode implantation in rodent models using the eLab/ePulse electrophysiology system for recording and stimulation purposes to assess neuronal functionality and synaptic plasticity. The evaluation parameters included the input/output function (IO), paired-pulse facilitation or depression (PPF/PPD), long-term potentiation (LTP), and long-term depression (LTD). • Provides a detailed picture-guided procedure for conducting in vivo stereotaxic neurosurgery. • Specifically covers the insertion of hippocampal electrodes and the recording of evoked extracellular field potentials. © 2023 The Author(s)
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