Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Comparative Study of Crh Microinjections Into Pvn and Cea Nuclei on Food Intake, Ghrelin, Leptin, and Glucose Levels in Acute Stressed Rats Publisher



Radahmadi M1 ; Izadi MS1 ; Rayatpour A1 ; Ghasemi M1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Basic and Clinical Neuroscience Published:2021


Abstract

Introduction: Corticotropin-Releasing Hormone (CRH) is involved in stress and energy homeostasis. On the other hand, CRH receptors also exist within the paraventricular nucleus (PVN) and Central Amygdala (CeA) nuclei. The present study compared the effect of CRH microinjections into PVN and CeA on three consecutive hours and cumulative food intake, internal regulatory factors of food intake, such as serum leptin and ghrelin, as well as blood glucose levels in rats under different acute psychological (Social Stress [SS] and Isolation Stress [IS] group) stresses. Methods: Sixty-six male Wistar rats were randomly allocated to 11 groups: Control, Sham, CRH-PVN, CRH-CeA, SS, IS, SS-CRH-PVN, SS-CRH-CeA, IS-CRH-PVN, and IS-CRH-CeA groups. The CRH (2 µg/kg in 0.5 µL saline) was injected into PVN and CeA nuclei in rats under everyday, acute social stress and isolation stress conditions. Results: Acute isolation and social stresses did not affect cumulative food intake. Whereas isolation stress led to changes in both leptin and glucose levels, social stress reduced only glucose levels. Cumulative food intake significantly decreased under acute CRH injection into the CeA and particularly into the PVN. Blood glucose significantly reduced in all the groups receiving CRH into their CeA. Conclusion: The PVN played a more important role compared to CeA on food intake. These nuclei probably employ different mechanisms for their effects on food intake. Besides, it seems that exogenously CRH injection into the PVN probably had a more anorectic effect than naturally activated CRH by stresses. Acute isolation stress had a greater impact than social stress on leptin level and cumulative food intake. Thus, elevated food intake related to leptin compared to ghrelin and glucose levels in the CRH-PVN group under acute social stress. © 2021 Iran University of Medical Sciences. All rights reserved.
Related Docs
View other Related Docs
1. Effects of Sub-Chronic Crh Administration Into the Hypothalamic Paraventricular and Central Amygdala Nuclei in Male Rats With a Focus on Food Intake Biomarkers, Anais da Academia Brasileira de Ciencias (2023)
2. Effect of Intraparaventricular Administration of Corticotropin Releasing Hormone on Food Intake in Food-Deprived Rats, Journal of Isfahan Medical School (2017)
3. Effect of Repeated Administration of Corticotropin-Releasing Hormone (Crh) in Central Amygdala Nucleus on Feeding Behavior in Adult Male Rats, Journal of Isfahan Medical School (2017)
4. The Effects of Subchronic Social and Isolation Stresses on Learning and Memory Trend in Male Rats, Physiology and Pharmacology (Iran) (2018)
5. The Protective Effects of Crocin on Input-Output Functions and Long-Term Potentiation of Hippocampal Ca1 Area in Rats Exposed to Chronic Social Isolated Stress, Basic and Clinical Neuroscience (2022)
6. Vitamin C Protects Against Chronic Social Isolation Stress-Induced Weight Gain and Depressive-Like Behavior in Adult Male Rats, Endocrine Regulations (2020)
7. Overview of the Central Amygdala Role in Feeding Behaviour, British Journal of Nutrition (2022)
8. Comparing Various Types of Chronic Psychological Stress on Cognitive Functions and Behaviors in Rats, Basic and Clinical Neuroscience (2024)
Experts (# of related papers)
View all Related Experts
Maryam Radahmadi (17)
Maedeh Ghasemi (7)
Other Related Docs
9. Effects of Chronic Scheduled–Caloric Dietary on Ghrelin Secretion and Food Intake in Adult Male Rats, Physiology and Pharmacology (Iran) (2019)
10. The Protective Effects of Escitalopram on Chronic Restraint Stress-Induced Memory Deficits in Adult Rats, Physiology and Pharmacology (Iran) (2022)
11. The Effect of Basolateral Amygdala Nucleus Lesion on Memory Under Acute, Mid and Chronic Stress in Male Rats, Turkish Journal of Medical Sciences (2016)
12. The Impact of Different Dark Chocolate Dietary Patterns on Synaptic Potency and Plasticity in the Hippocampal Ca1 Area of the Rats Under Chronic Isolation Stress, Nutritional Neuroscience (2023)
13. Involvement of Cholinergic Pathway in Energy Homeostasis Via Ventromedial Hypothalamic D2 Receptors, Journal of Isfahan Medical School (2019)
14. Effect of Psychological Distress on Weight Concern and Weight Control Behaviors, Archives of Iranian Medicine (2014)
15. Comparing the Effects of Crocin at Different Doses on Excitability and Long-Term Potentiation in the Ca1 Area, As Well As the Electroencephalogram Responses of Rats Under Chronic Stress, Metabolic Brain Disease (2021)
16. Effects of Different Timing of Stress on Corticosterone, Bdnf and Memory in Male Rats, Physiology and Behavior (2015)
17. Effects of Aqueous Saffron Extract on Glucoregulation As Well As Hepatic Agt and Tnf‑Α Gene Expression in Rats Subjected to Sub‑Chronic Stress, Advanced Biomedical Research (2023)
18. Comparative Study of the Protective Effects of Crocin and Exercise on Long-Term Potentiation of Ca1 in Rats Under Chronic Unpredictable Stress, Life Sciences (2020)
19. The Impact of Crocin and Chronic Isolation Stress on Passive Avoidance Memory and Brain Electrical Activity in Male Rats, Physiology and Pharmacology (Iran) (2022)
20. Effects of Stress on Exacerbation of Diabetes Mellitus, Serum Glucose and Cortisol Levels and Body Weight in Rats, Pathophysiology (2006)