Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Identification and Validation of Nf-Kb Pathway-Related Lncrna Upregulated in Ibd Patients Publisher



Kazemifard N1, 2 ; Kazemi M1, 4 ; Shahrokh S3 ; Aghdaei HA2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  3. 3. Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  4. 4. Pediatric Inherited Diseases Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Gene Reports Published:2023


Abstract

Background: Inflammatory bowel disease (IBD) is a complex disease classified into two types, ulcerative colitis (UC) and Crohn's disease (CD), and it results from the interaction between genetic and environmental factors influencing immune responses. The nuclear factor kappa beta (NF-κB) pathway is one of the major regulators of inflammatory responses associated with the pathogenesis of IBD. lncRNAs, involved in immune responses were suggested to associate with IBD pathogenesis and maintenance of intestinal mucosa function via the interaction network between miRNAs, transcription factors, and mRNAs. Method: In this study, we aimed to predict regulatory lncRNAs for the NF-κB pathway with bioinformatics studies and validate the expression of 4 predicted lncRNAs (NRAV, HULC, RAD51-AS1, and SBF2-AS1) in blood and tissue samples of UC patients. Results: The qPCR results indicated that NRAV and HULC are upregulated in UC colonic samples, and RAD51-AS1 and SBF2-AS1 are upregulated in blood samples of UC patients compared to controls. Conclusion: This study indicated that bioinformatics studies could successfully predict disease-related lncRNAs and studied lncRNAs such as HULC, NRAV, RAD51-AS1 and SBF2-AS1 may have and important role in controlling inflammation in IBD. © 2023
Related Docs
View other Related Docs
1. Emerging Involvement of Long Non-Coding Rnas in Gastrointestinal Associated Inflammatory Disorders, Comparative Immunology, Microbiology and Infectious Diseases (2020)
2. A Prediction of the Crnde Role by Modulating Nf-Κb Pathway in Inflammatory Bowel Disease (Ibd), Biochemistry and Biophysics Reports (2024)
3. Microrna Binding Site Polymorphism in Inflammatory Genes Associated With Colorectal Cancer: Literature Review and Bioinformatics Analysis, Cancer Gene Therapy (2020)
4. Alteration of Mir-21, Mir-433 and Mir-590 Tissue Expression Related to the Tgf-Β Signaling Pathway in Ulcerative Colitis Patients, Archives of Physiology and Biochemistry (2022)
5. Ibd Genomic Risk Loci and Overlap With Other Inflammatory Diseases, Molecular Genetics of Inflammatory Bowel Disease (2019)
6. Novel Biomarkers for Neoplastic Progression From Ulcerative Colitis to Colorectal Cancer: A Systems Biology Approach, Scientific Reports (2023)
7. Decreased Expression of Hsa-Mir-142-3P and Hsa-Mir-155-5P in Common Variable Immunodeficiency and Involvement of Their Target Genes and Biological Pathways, Allergologia et Immunopathologia (2025)
8. Single Nucleotide Polymorphism Rs10889677 in Mirnas Let-7E and Let-7F Binding Site of Il23r Gene Is a Strong Colorectal Cancer Determinant: Report and Meta-Analysis, Cancer Genetics (2019)
Experts (# of related papers)
View all Related Experts
Rasoul Salehi (5)
Mohammad Kazemi (3)
Other Related Docs
9. Oleuropein As an Effective Suppressor of Inflammation and Microrna-146A Expression in Patients With Rheumatoid Arthritis, Cell Journal (2023)
10. New Long Noncoding Rna Biomarkers and Cerna Networks on Mir‑616‑3P in Colorectal Cancer: Bioinformatics‑Based Study, Journal of Research in Medical Sciences (2024)
11. Mir-145 and Mir20a-5P Potentially Mediate Pleiotropic Effects of Interferon-Beta Through Mitogen-Activated Protein Kinase Signaling Pathway in Multiple Sclerosis Patients, Journal of Molecular Neuroscience (2017)
12. Alteration of the Inflammatory and Anti-Inflammatory Cytokine Profiles of Peripheral Blood Mononuclear Cell in Crohn’S Disease Patients After Following Up, Iranian Journal of Public Health (2022)
13. Mirna-Binding Site Polymorphism In Il-15Ra Gene in Rheumatoid Arthritis and Systemic Lupus Erythematosus: Correlation With Disease Risk and Clinical Characteristics, Clinical Rheumatology (2022)
14. Investigation of Rs531564 Polymorphism in the Primary Microrna-124 Gene in Patients With Systemic Lupus Erythematosus and Rheumatoid Arthritis: Association With Disease Susceptibility and Clinical Characteristics, Iranian Journal of Allergy, Asthma and Immunology (2021)
15. Identification of Genes Related to Ribosomal Proteins in Colorectal Cancer: Exploring Their Potential As Biomarkers, Prognostic Indicators, and Therapeutic Targets, Molecular Biology Reports (2024)
16. Integrative Analysis of Lncrnas in Th17 Cell Lineage to Discover New Potential Biomarkers and Therapeutic Targets in Autoimmune Diseases, Molecular Therapy Nucleic Acids (2018)
17. Leng8-As1: A Prognostic Biomarker in Colorectal Cancer-Differential Expression and Clinical Implications, Indian Journal of Clinical Biochemistry (2024)
18. Comparison of Interleukin 18 Gene Expression and Its Serum Level Between Iranian Colorectal Cancer (Crc) Patients and Healthy People, Biologia (2019)
19. Impact of Mirna-Binding Site Polymorphisms in Stat3 Gene on Occurrence and Clinical Characteristics of Systemic Lupus Erythematosus, Lupus (2022)
20. Connection of Mir-185 and Mir-320A Expression Levels With Response to Interferon-Beta in Multiple Sclerosis Patients, Multiple Sclerosis and Related Disorders (2020)
21. Locked Nucleic Acid Anti-Mir-21 Inhibits Cell Growth and Invasive Behaviors of a Colorectal Adenocarcinoma Cell Line: Lna-Anti-Mir As a Novel Approach, Cancer Gene Therapy (2016)
22. Exploring Conserved Mrna-Mirna Interactions in Colon and Lung Cancers, Gastroenterology and Hepatology from Bed to Bench (2017)
23. An Integrated Bioinformatics Analysis of the Potential Regulatory Effects of Mir-21 on T-Cell Related Target Genes in Multiple Sclerosis, Avicenna Journal of Medical Biotechnology (2021)
24. Mir-196: Emerging of a New Potential Therapeutic Target and Biomarker in Colorectal Cancer, Molecular Biology Reports (2020)
25. Current Status and Perspectives Regarding Lna-Anti-Mir Oligonucleotides and Microrna Mir-21 Inhibitors As a Potential Therapeutic Option in Treatment of Colorectal Cancer, Journal of Cellular Biochemistry (2017)
26. Emerging Roles for Lncrna-Neat1 in Colorectal Cancer, Cancer Cell International (2022)
27. Lncrna–Mirna–Mrna Networks of Gastrointestinal Cancers Representing Common and Specific Lncrnas and Mrnas, Frontiers in Genetics (2022)
28. The Importance of Snps at Mirna Binding Sites As Biomarkers of Gastric and Colorectal Cancers: A Systematic Review, Journal of Personalized Medicine (2022)
29. Exploring the Role of Interleukin 11 in Cancer Progression, Patient Survival, and Therapeutic Insights, Molecular Biology Reports (2024)
30. Dysregulated of the Fam138e and Clrn1-As1 Lncrnas Could Be Diagnosis Biomarkers in Colorectal Cancer, Human Gene (2024)
31. Lncarod Promotes the Proliferation and Migration of Gastric Cancer: A Bioinformatics Analysis and Experimental Validation, Functional and Integrative Genomics (2023)
32. Study of the Correlation Between Mir-106A, Mir-125B, and Mir-330 on Multiple Sclerosis Patients by Targeting Tnfsf4 and Sp1 in Nf-Κb/Tnf-Α Pathway: A Case-Control Study, Cell Journal (2022)
33. A Functional Microrna Binding Site Variant in Il-23R Gene in Systemic Lupus Erythematosus and Rheumatoid Arthritis: Is There Any Correlation?, Molecular Biology Reports (2022)
34. An Analysis and Survey of Interleukin-10 Receptor Mutation in Inflammatory Bowel Disease (Ibd) in the First Iranian Ibd Cohort, Journal of Laboratory Medicine (2019)
35. Micrornas Regulating Wnt Signaling Pathway in Colorectal Cancer: Biological Implications and Clinical Potentials, Functional and Integrative Genomics (2022)
36. Assessment of Microrna-146A in Generalized Aggressive Periodontitis and Its Association With Disease Severity, Journal of Periodontal Research (2019)
37. Single Nucleotide Polymorphism Rs696 in Mir449a Binding Site of Nfkbia Gene Is Correlated With Risk of Colorectal Cancer, Gastroenterology and Hepatology from Bed to Bench (2018)
38. Importance of Stat3 Polymorphisms on the Risk and Clinical Characteristics of Rheumatoid Arthritis, Iranian Journal of Allergy, Asthma and Immunology (2022)
39. Therapeutic Potential of Mesenchymal Stem/Stromal Cells (Mscs)-Based Cell Therapy for Inflammatory Bowel Diseases (Ibd) Therapy, European Journal of Medical Research (2023)
40. Inflammatory Cytokines and Oxidative Stress Biomarkers in Irritable Bowel Syndrome: Association With Digestive Symptoms and Quality of Life, Cytokine (2017)
41. Genomic Testing Identifies Monogenic Causes in Patients With Very Early-Onset Inflammatory Bowel Disease: A Multicenter Survey in an Iranian Cohort, Clinical and Experimental Immunology (2024)
42. Emerging Circulating Mirnas and Lncrnas in Upper Gastrointestinal Cancers, Expert Review of Molecular Diagnostics (2020)
43. Association of a Mirna-Binding Site Polymorphism in Il-16 Gene With Disease Risk and Clinical Characteristics of Rheumatoid Arthritis and Systemic Lupus Erythematosus, Clinical Rheumatology (2022)
44. Very Early Onset Inflammatory Bowel Disease: Investigation of the Il-10 Signaling Pathway in Iranian Children, European Journal of Medical Genetics (2017)
45. Construction of a Three-Component Regulatory Network of Transcribed Ultraconserved Regions for the Identification of Prognostic Biomarkers in Gastric Cancer, Journal of Cellular Biochemistry (2023)
46. Microrna-29B Variants and Mxa Expression Change During Interferon Beta Therapy in Patients With Relapsing-Remitting Multiple Sclerosis, Multiple Sclerosis and Related Disorders (2019)
47. Rs12904 Polymorphism in the 3'-Untranslated Region of Ephrin A1 Ligand and the Risk of Sporadic Colorectal Cancer in the Iranian Population, Journal of Cancer Research and Therapeutics (2019)
48. Unraveling the Molecular Landscape of Osteoarthritis: A Comprehensive Review Focused on the Role of Non-Coding Rnas, Pathology Research and Practice (2024)
49. Construction of a Cell Cycle-Specific Lncrna-Mirna-Mrna Network Reveals Novel Key Lncrnas in Colorectal Cancer, Biomarkers (2024)
50. The Pathogenic Roles of Lncrna-Taurine Upregulated 1 (Tug1) in Colorectal Cancer, Cancer Cell International (2022)