Epi/Perineural and Schwann Cells As Well As Perineural Sheath Integrity Are Affected Following 2,4-D Exposure

Epi/Perineural and Schwann Cells As Well As Perineural Sheath Integrity Are Affected Following 2,4-D Exposure Publisher Pubmed

Sharifi Pasandi M^{1, 2} ; Hosseini Shirazi F^{3, 4} ; Gholami MR⁵ ; Salehi H⁶ ; Najafzadeh N⁷ ; Mazani M² ; Ghasemi Hamidabadi H⁸ ; Niapour A⁷

Show Affiliations

1. Student Research Committee, Ardabil University of Medical Sciences, Ardabil, Iran
2. Department of Biochemistry, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
3. Department of Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4. Pharmaceutical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5. Department of Anatomical Sciences, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
6. Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
7. Research Laboratory for Embryology and Stem Cells, Department of Anatomical Sciences, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
8. Immunogenetic Research Center, Department of Anatomy and Cell Biology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

Source: Neurotoxicity Research Published:2017

Abstract

2,4-dicholorophenoxy acetic acid (2,4-D) is a worldwide-known hormone herbicide. However, there are increasing concerns about its exposure and risks of developing pathological conditions for the peripheral nervous system. The aim of this study was to investigate the mechanism(s) involved in the toxicity of 2,4-D on peripheral nerve’s cellular components. The epi/perineural and Schwann cells and a total of three cell lines were treated with 2,4-D. The viability of cells at different doses of 2,4-D was measured by MTT assay. The cell cycle analyses, cumulative cell counting, fluorescent staining, antioxidant and caspase enzymes activity were examined on epi/perineural and Schwann cells. The epi/perineural cells were assessed as having biological macromolecular changes. Some tight junction-related genes and proteins were also tested on explants of 2,4-D treated epi/perineural tissue. The viability of 2,4-D treated cells was reduced in a dose-dependent manner. Reduced growth rate and G1 cell cycle arrest were verified in 2,4-D treated epi/perineural and Schwann cells. The use of staining methods (acridine orange/ethidium bromide and DAPI) and caspase 3/7 activity assay along with malondialdehyde, glutathione peroxidase, and superoxide dismutase activity assays indicated the apoptotic and oxidant effects of 2,4-D on epi/perineural and Schwann cells. Data obtained from FTIR revealed changes in epi/perineural proteins and cell membrane lipids. Additionally, claudin-1, occludin, and ZO-1 gene/protein expression profiles were significantly reduced in 2,4-D-treated epi/perineural pieces. Our data indicated that oxidative stress, apoptosis of epi/perineural and Schwann cell and impaired blood-nerve barrier may have contributed to nerve damage following 2,4-D exposure. © 2017, Springer Science+Business Media, LLC.

2. Toxicity Effects of Methamphetamine on Embryonic Stem Cell-Derived Neuron, Journal of Research in Medical Sciences (2012)

3. All Trans Retinoic Acid Modulates Peripheral Nerve Fibroblasts Viability and Apoptosis, Tissue and Cell (2015)

Experts (# of related papers)

Hossein Salehi (2)

Rokhsareh Meamar (1)

Style	Citing Format
MLA	Sharifi Pasandi M, et al.. "Epi/Perineural and Schwann Cells As Well As Perineural Sheath Integrity Are Affected Following 2,4-D Exposure." Neurotoxicity Research, vol. 32, no. 4, 2017, pp. 624-638.
APA	Sharifi Pasandi M, Hosseini Shirazi F, Gholami MR, Salehi H, Najafzadeh N, Mazani M, Ghasemi Hamidabadi H, Niapour A (2017). Epi/Perineural and Schwann Cells As Well As Perineural Sheath Integrity Are Affected Following 2,4-D Exposure. Neurotoxicity Research, 32(4), 624-638.
Chicago	Sharifi Pasandi M, Hosseini Shirazi F, Gholami MR, Salehi H, Najafzadeh N, Mazani M, Ghasemi Hamidabadi H, Niapour A. "Epi/Perineural and Schwann Cells As Well As Perineural Sheath Integrity Are Affected Following 2,4-D Exposure." Neurotoxicity Research 32, no. 4 (2017): 624-638.
Harvard	Sharifi Pasandi M et al. (2017) 'Epi/Perineural and Schwann Cells As Well As Perineural Sheath Integrity Are Affected Following 2,4-D Exposure', Neurotoxicity Research, 32(4), pp. 624-638.
Vancouver	Sharifi Pasandi M, Hosseini Shirazi F, Gholami MR, Salehi H, Najafzadeh N, Mazani M, et al.. Epi/Perineural and Schwann Cells As Well As Perineural Sheath Integrity Are Affected Following 2,4-D Exposure. Neurotoxicity Research. 2017;32(4):624-638.
BibTex	@article{ author = {Sharifi Pasandi M and Hosseini Shirazi F and Gholami MR and Salehi H and Najafzadeh N and Mazani M and Ghasemi Hamidabadi H and Niapour A}, title = {Epi/Perineural and Schwann Cells As Well As Perineural Sheath Integrity Are Affected Following 2,4-D Exposure}, journal = {Neurotoxicity Research}, volume = {32}, number = {4}, pages = {624-638}, year = {2017} }
RIS	TY - JOUR AU - Sharifi Pasandi M AU - Hosseini Shirazi F AU - Gholami MR AU - Salehi H AU - Najafzadeh N AU - Mazani M AU - Ghasemi Hamidabadi H AU - Niapour A TI - Epi/Perineural and Schwann Cells As Well As Perineural Sheath Integrity Are Affected Following 2,4-D Exposure JO - Neurotoxicity Research VL - 32 IS - 4 SP - 624 EP - 638 PY - 2017 ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract