The Relationship Between Transcript Expression Levels of Nuclear Encoded (Tfam, Nrf1) and Mitochondrial Encoded (Mt-Co1) Genes in Single Human Oocytes During Oocyte Maturation

The Relationship Between Transcript Expression Levels of Nuclear Encoded (Tfam, Nrf1) and Mitochondrial Encoded (Mt-Co1) Genes in Single Human Oocytes During Oocyte Maturation Publisher

Ghaffari Novin M¹ ; Allahveisi A² ; Noruzinia M³ ; Farhadifar F⁴ ; Yousefian E⁵ ; Dehghani Fard A⁶ ; Salimi M¹

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1. Department of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Science, Tehran, Iran
2. Department of Anatomical Sciences, Faculty of Medicine, Kurdistan University of Medical Sciences, Pasdaran Street, Sanandaj, Iran
3. Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
4. Deptartment of Obstetrics and Gynecology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
5. Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
6. Sarem Cell Research Center, Sarem Women's Hospital, Tehran, Iran

Source: Balkan Journal of Medical Genetics Published:2015

Abstract

In some cases of infertility in women, human oocytes fail to mature when they reach the metaphase II (MII) stage. Mitochondria plays an important role in oocyte maturation. A large number of mitochondrial DNA (mtDNA), copied in oocytes, is essential for providing adenosine triphosphate (ATP) during oocyte maturation. The purpose of this study was to identify the relationship between transcript expression levels of the mitochondrial encoded gene (MT-CO1) and two nuclear encoded genes, nuclear respiratory factor 1 (NRF1) and mitochondrial transcription factor A (TFAM) in various stages of human oocyte maturation. Nine consenting patients, age 21-35 years old, with male factors were selected for ovarian stimulation and intracytoplasmic sperm injection (ICSI) procedures. mRNA levels of mitochondrial-related genes were performed by singlecell TaqMan® quantitative real-time polymerase chain reaction (qRT-PCR). There was no significant relationship between the relative expression levels in germinal vesicle (GV) stage oocytes (p = 0.62). On the contrary, a significant relationship was seen between the relative expression levels of TFAM and NRF1 and the MT-CO1 genes at the stages of metaphase I (MI) and MII (p = 0.03 and p = 0.002). A relationship exists between the transcript expression levels of TFAM and NRF1, and MT-CO1 genes in various stages of human oocyte maturation. © 2016 Balkan Journal of Medical Genetics.

2. Altered Expression of Klc3 May Affect Semen Parameters, International Journal of Reproductive BioMedicine (2016)

Experts (# of related papers)

Mohammad Mardani (1)

Shahnaz Razavi (1)

Style	Citing Format
MLA	Ghaffari Novin M, et al.. "The Relationship Between Transcript Expression Levels of Nuclear Encoded (Tfam, Nrf1) and Mitochondrial Encoded (Mt-Co1) Genes in Single Human Oocytes During Oocyte Maturation." Balkan Journal of Medical Genetics, vol. 18, no. 1, 2015, pp. 39-46.
APA	Ghaffari Novin M, Allahveisi A, Noruzinia M, Farhadifar F, Yousefian E, Dehghani Fard A, Salimi M (2015). The Relationship Between Transcript Expression Levels of Nuclear Encoded (Tfam, Nrf1) and Mitochondrial Encoded (Mt-Co1) Genes in Single Human Oocytes During Oocyte Maturation. Balkan Journal of Medical Genetics, 18(1), 39-46.
Chicago	Ghaffari Novin M, Allahveisi A, Noruzinia M, Farhadifar F, Yousefian E, Dehghani Fard A, Salimi M. "The Relationship Between Transcript Expression Levels of Nuclear Encoded (Tfam, Nrf1) and Mitochondrial Encoded (Mt-Co1) Genes in Single Human Oocytes During Oocyte Maturation." Balkan Journal of Medical Genetics 18, no. 1 (2015): 39-46.
Harvard	Ghaffari Novin M et al. (2015) 'The Relationship Between Transcript Expression Levels of Nuclear Encoded (Tfam, Nrf1) and Mitochondrial Encoded (Mt-Co1) Genes in Single Human Oocytes During Oocyte Maturation', Balkan Journal of Medical Genetics, 18(1), pp. 39-46.
Vancouver	Ghaffari Novin M, Allahveisi A, Noruzinia M, Farhadifar F, Yousefian E, Dehghani Fard A, et al.. The Relationship Between Transcript Expression Levels of Nuclear Encoded (Tfam, Nrf1) and Mitochondrial Encoded (Mt-Co1) Genes in Single Human Oocytes During Oocyte Maturation. Balkan Journal of Medical Genetics. 2015;18(1):39-46.
BibTex	@article{ author = {Ghaffari Novin M and Allahveisi A and Noruzinia M and Farhadifar F and Yousefian E and Dehghani Fard A and Salimi M}, title = {The Relationship Between Transcript Expression Levels of Nuclear Encoded (Tfam, Nrf1) and Mitochondrial Encoded (Mt-Co1) Genes in Single Human Oocytes During Oocyte Maturation}, journal = {Balkan Journal of Medical Genetics}, volume = {18}, number = {1}, pages = {39-46}, year = {2015} }
RIS	TY - JOUR AU - Ghaffari Novin M AU - Allahveisi A AU - Noruzinia M AU - Farhadifar F AU - Yousefian E AU - Dehghani Fard A AU - Salimi M TI - The Relationship Between Transcript Expression Levels of Nuclear Encoded (Tfam, Nrf1) and Mitochondrial Encoded (Mt-Co1) Genes in Single Human Oocytes During Oocyte Maturation JO - Balkan Journal of Medical Genetics VL - 18 IS - 1 SP - 39 EP - 46 PY - 2015 ER -

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