Optimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase

Optimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase Publisher

Abedi D¹ ; Fazeli MR² ; Jafarian A¹

Source: Iranian Journal of Pharmaceutical Research Published:2004

Abstract

Penicillin G acylase from E. coli TA1 was immobilized by Cross-Linked Enzyme Aggregates (CLEA), a new method for immobilization. This biocatalyst and commercial immobilized penicillin G acylase (PGA-450) were used to study the effect of pH, temperature and substrate concentration on the synthesis of ampicillin from phenyl glycine methyl ester (PGME) and 6-aminopenicillanic acid (6-APA). Compared with PGA-450, this immobilized enzyme showed a high synthesis activity. The optimum conditions for synthetic activity was at pH 6, 25°C and 2:6 (6-APA:PGME) substrate ratio. © 2004 by School of Pharmacy Shaheed Beheshti University of Medical Sciences and Health Services.

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Style	Citing Format
MLA	Abedi D, Fazeli MR, Jafarian A. "Optimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase." Iranian Journal of Pharmaceutical Research, vol. 3, no. 3, 2004, pp. 159-164.
APA	Abedi D, Fazeli MR, Jafarian A (2004). Optimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase. Iranian Journal of Pharmaceutical Research, 3(3), 159-164.
Chicago	Abedi D, Fazeli MR, Jafarian A. "Optimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase." Iranian Journal of Pharmaceutical Research 3, no. 3 (2004): 159-164.
Harvard	Abedi D, Fazeli MR, Jafarian A (2004) 'Optimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase', Iranian Journal of Pharmaceutical Research, 3(3), pp. 159-164.
Vancouver	Abedi D, Fazeli MR, Jafarian A. Optimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase. Iranian Journal of Pharmaceutical Research. 2004;3(3):159-164.
BibTex	@article{ author = {Abedi D and Fazeli MR and Jafarian A}, title = {Optimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase}, journal = {Iranian Journal of Pharmaceutical Research}, volume = {3}, number = {3}, pages = {159-164}, year = {2004} }
RIS	TY - JOUR AU - Abedi D AU - Fazeli MR AU - Jafarian A TI - Optimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase JO - Iranian Journal of Pharmaceutical Research VL - 3 IS - 3 SP - 159 EP - 164 PY - 2004 ER -

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