A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (Cop-Gfp) in Baculovirus Expression System

A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (Cop-Gfp) in Baculovirus Expression System Pubmed

Shokrollahi N¹ ; Shahbazzadeh D¹ ; Pooshangbagheri K¹ ; Habibianbouhi M² ; Jahaniannajafabadi A³ ; Behdani M¹

Show Affiliations

1. Biotechnology Research Center, Venom and Biotherapeutics Molecules Lab, Pasteur Institute of Iran, Tehran, Iran
2. National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
3. Department of Pharmaceutical Biotechnology, School of Pharmacy, Isfahan University of Medical Sciences and Health Services, Isfahan, Iran

Source: Iranian Biomedical Journal Published:2016

Abstract

Background: Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. Methods: The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluorescent protein (copGFP). In this light, the resultant vector was constructed and used for transfection of Spodoptera frugiperda cells. Results: Expression of the copGFP protein in insect cells was confirmed by fluorescent microscopy and Western-blot analysis. Conclusion: The application of copGFP control bacmid can be considered as an appropriate control for insect cell transfection. © 2016, Pasteur Institute of Iran. All rights reserved.

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Experts (# of related papers)

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Style	Citing Format
MLA	Shokrollahi N, et al.. "A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (Cop-Gfp) in Baculovirus Expression System." Iranian Biomedical Journal, vol. 20, no. 3, 2016, pp. 182-186.
APA	Shokrollahi N, Shahbazzadeh D, Pooshangbagheri K, Habibianbouhi M, Jahaniannajafabadi A, Behdani M (2016). A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (Cop-Gfp) in Baculovirus Expression System. Iranian Biomedical Journal, 20(3), 182-186.
Chicago	Shokrollahi N, Shahbazzadeh D, Pooshangbagheri K, Habibianbouhi M, Jahaniannajafabadi A, Behdani M. "A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (Cop-Gfp) in Baculovirus Expression System." Iranian Biomedical Journal 20, no. 3 (2016): 182-186.
Harvard	Shokrollahi N et al. (2016) 'A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (Cop-Gfp) in Baculovirus Expression System', Iranian Biomedical Journal, 20(3), pp. 182-186.
Vancouver	Shokrollahi N, Shahbazzadeh D, Pooshangbagheri K, Habibianbouhi M, Jahaniannajafabadi A, Behdani M. A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (Cop-Gfp) in Baculovirus Expression System. Iranian Biomedical Journal. 2016;20(3):182-186.
BibTex	@article{ author = {Shokrollahi N and Shahbazzadeh D and Pooshangbagheri K and Habibianbouhi M and Jahaniannajafabadi A and Behdani M}, title = {A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (Cop-Gfp) in Baculovirus Expression System}, journal = {Iranian Biomedical Journal}, volume = {20}, number = {3}, pages = {182-186}, year = {2016} }
RIS	TY - JOUR AU - Shokrollahi N AU - Shahbazzadeh D AU - Pooshangbagheri K AU - Habibianbouhi M AU - Jahaniannajafabadi A AU - Behdani M TI - A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (Cop-Gfp) in Baculovirus Expression System JO - Iranian Biomedical Journal VL - 20 IS - 3 SP - 182 EP - 186 PY - 2016 ER -

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