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Molecular Detection of Lipase a Gene in Putative Bacillus Subtilis Strains Isolated From Soil



Mir Mohammad Sadeghi H1, 2 ; Rabbani M1 ; Moazen F1 ; Homami S1
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Authors Affiliations
  1. 1. Department of Biotechnology, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Iranian Journal of Biotechnology Published:2010

Abstract

The present study was undertaken to screen the soil samples collected in Iran for the presence of the Bacillus subtilis lipase A gene. The bacterial colonies obtained from the collected soil samples were examined by physical appearance, biochemical tests and the polymerase chain reaction (PCR). Only four colonies were identified as putative B. subtilis strains and all contained the lipase A gene. However, the intensities of the DNA bands were different and correlated with the differences obtained from the biochemical tests. Polymorphism of the lipase gene was also determined in samples using SSCP assay. In conclusion, this study demonstrates an easy and reliable method for detection of the lipase gene in B. subtilis strains. Further screening of the soil by this method will enable the detection and identification of industrially more favorable lipases.
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