A Report of a Novel Mutation in Human Prostacyclin Receptor Gene in Patients Affected With Migraine



Kheirollahi M¹ ; Pourreza MR¹ ; Khorvash F² ; Kazemi M¹ ; Amini G¹ Show Affiliations
Source: Iranian Journal of Psychiatry Published:2017

Abstract

Objective: The human prostacyclin receptor gene encodes the human prostacyclin receptor, which is a part of the vasodilator system, during migraine attacks and almost certainly plays an important role in the mechanism of this disease. Method: The present study aimed at determining any variants in PTGIR gene by means of PCR and direct sequencing. Blood samples were taken from the patients, and genomic DNA was extracted. Polymerase chain reaction was performed on the extracted DNA. The PCR products were then sequenced using the Sanger method. Results: When reviewing the familial and clinicopathological history of the 2 patients, we found that both had symptoms of migraine with visual aura and that their mothers were also suffering from migraine. Their parents were not relatives prior to marriage. Direct sequencing of Exon 2 of the PTGIR gene showed the presence of 2 mutations. These mutations were heterozygous and made the following changes: g.1626T > A, c.754T > A, cDNA.867T > A, and p. S252T for the first mutation, and c.753C > T, cDNA866C > T, g.1625C > T, and p. C251C for the second mutation. The first mutation altered the amino acid and was a novel mutation. The second change was a conservative mutation that has already been reported. Conclusion: The prediction results of silico studies indicated that the c.754T > A change would negatively affect the protein's function and seemed to cause the disease. However, functional analysis is required to confirm the association between the variant and the disease.