Another Case of Para-Bombay Phenotype in an Iranian Donor



Pourazar A¹ ; Joshi S² ; Clarke VA³ ; Ala F³ Show Affiliations
Source: Archives of Iranian Medicine Published:2004

Abstract

The blood specimen of a 42-year-old male donor was targeted for detailed study at the Iranian National Blood Transfusion Services as the ABO blood grouping of his cells and serum did not match. Preliminary results suggested that his group was Bombay (Oh) phenotype. For confirmation, his blood specimen was air-shipped to Bombay, where detailed studies revealed the presence of depressed A antigens on his red cells that were detectable with variable strength with anti-A+B reagents prepared from group O individuals. His red cells failed to show agglutination by anti-H reagents from Bombay phenotype, lectin from Ulex europaeus, and immunized chicken. The donor's serum agglutinated group B red cells showing a titer of up to 1:32 at room temperature, but reacted with A and O red cells only at 4°C in a low titer of 1:4 - 8. The reactivity against group A and O cells was partially inhibited by saliva from individuals secreting A/H antigens and was characterized as anti-I. The donor was classified as para-Bombay of AHw phenotype. The anti-I antigen strength on the donor's red cells, as determined by titration of anti-I and anti-i reagents, was comparable to normal adult individuals but not to the Bombay phenotype, thus supporting his phenotype as para-Bombay. This case provides another example of the para-Bombay phenotype that serologically differs from the one reported earlier in Iran.