Induced in Vitro Differentiation of Neural-Like Cells From Human Exfoliated Deciduous Teeth-Derived Stem Cells Publisher Pubmed



Nourbakhsh N¹ ; Soleimani M² ; Taghipour Z² ; Karbalaie K³ ; Mousavi S⁴ ; Talebi A⁵ ; Nadali F⁵ ; Tanhaei S³ ; Kiyani GA¹ ; Nematollahi M³ ; Rabiei F¹ ; Mardani M² ; Bahramiyan H² ; Torabinejad M⁶ Show All Authors Show Affiliations
Source: International Journal of Developmental Biology Published:2011

Abstract

Stem cells from human exfoliated deciduous teeth (SHED) are highly proliferative, clonogenic and multipotent stem cells with a neural crest cell origin. Additionally, they can be collected with minimal invasiveness in comparison with other sources of mesenchymal stem cells (MSCs). Therefore, SHED could be a desirable option for potential therapeutic applications. In this study, SHEDs were established from enzyme-disaggregated deciduous dental pulp obtained from 6 to 9 year-old children. The cells had typical fibroblastoid morphology and expressed antigens characteristic of MSCs, STRO1, CD146, CD45, CD90, CD106 and CD166, but not the hematopoietic and endothelial markers, CD34 and CD31, as assessed by FACS analysis. Differentiation assessment revealed a strong osteogenic and adipogenic potential of SHEDs. In order to further evaluate the in vitro differentiation potential of SHED into neural cells, a simple short time growth factormediated induction was used. Immunofluorescence staining and flow cytometric analysis revealed that SHED rapidly expressed nestin and β-III tubulin, and later expressed intermediate neural markers. In addition, the intensity and percentages of nestin and β-III tubulin and mature neural markers (PSA-NCAM, NeuN, Tau, TH, or GFAP) increased significantly following treatment. Moreover, RT-PCR and Western blot analyses showed that the neural markers were strongly upregulated after induction. In conclusion, these results provide evidence that SHED can differentiate into neural cells by the expression of a comprehensive set of genes and proteins that define neural-like cells in vitro. SHED cells might be considered as new candidates for the autologous transplantation of a wide variety of neurological diseases and neurotraumatic injuries. © 2011 UBC Press.