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Comparative Analysis and Identification of Spike Mutations in Iranian Covid-19 Samples From the First Three Waves of Disease Publisher



Shoaei P1 ; Ranjbar M2 ; Tokhanbigli S3 ; Ataei B3 ; Alibakhshi A4 ; Haghjooy Javanmard S5 ; Ahangarzadeh S3
Authors
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Authors Affiliations
  1. 1. Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, Iran
  3. 3. Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Molecular Medicine Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
  5. 5. Department of Physiology, Applied Physiology Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Advanced Biomedical Research Published:2023


Abstract

Background: The spike surface glycoprotein of SARS-CoV-2 is the essential protein in virus attachment to the target cell and cell entrance. As this protein contains immunodominant epitopes and is the main target for immune recognition, it is the critical target for vaccine and therapeutics development. In the current research, we analyzed the variability and mutations of the spike glycoprotein isolated from 72 COVID-19-positive patients from Iran's first three waves of disease. Materials and Methods: The RNA was extracted from nasopharyngeal samples of confirmed COVID-19 cases and served as a template for cDNA synthesis and reverse transcriptase polymerase chain reaction. The reverse transcriptase polymerase chain reaction products of each sample were assembled and sequenced. Results: After analysis of 72 sequences, we obtained 46 single nucleotide polymorphisms, including 23 that produce amino acid changes. Our analysis showed that the most frequent mutation was the D614G (in the samples of the second and third waves). Conclusions: Our findings suggest that developing effective vaccines requires identifying the predominant variants of SARS-CoV-2 in each community. © 2023 Medknow. All rights reserved.
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