Effects of Methadone on the Toll-Like Receptor 4 Expression in Human Non-Small Cell Lung Carcinoma A549 Cell Line Using In-Silico and in Vitro Techniques

Effects of Methadone on the Toll-Like Receptor 4 Expression in Human Non-Small Cell Lung Carcinoma A549 Cell Line Using In-Silico and in Vitro Techniques Publisher

Vaseghi G¹ ; Rashidi N² ; Zare N³ ; Ghasemi F⁴ ; Pourhadi M² ; Rafiee L² ; Javanmard SH^{2, 3}

Show Affiliations

1. Isfahan Cardiovascular Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran
2. Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3. Applied Physiology Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran
4. Department of Bioinformatics and Systems Biology, School Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Advanced Biomedical Research Published:2022

Abstract

Background: In this study, the effects of methadone and naloxone on the expression of toll-like receptor 4 (TLR4) gene have been evaluated in human non-small cell lung carcinoma A549 cell line migration using in-silico and in vitro techniques. Materials and Methods: Lung cancer A549 cell cultures were stimulated for 24 h with methadone (5, 10, and 20 µM) and naloxone (20 and 40 µM) concentrations. The level of TLR4 expression was determined by the quantitative real-time polymerase chain reaction. Migration of the A549 cells was investigated after a 4-h incubation period with methadone using the Boyden Chamber assay. Results: Migration rate of the A549 cells treated with 5 (P < 0.05) and 20 (P < 0.01) µM methadone was, respectively, increased and decreased with 20 µM naloxone (P < 0.05). Furthermore, the TLR4 expression was enhanced with 5 (P < 0.05) and 20 (P < 0.01) µM methadone and decreased with 20 (P < 0.05) and 40 µM naloxone (P < 0.01). In addition, in silico docking analysis revealed docking of methadone to MD-2 and TLR4. Conclusion: According to the present DATA, methadone affects the TLR4 expression. It may however cause adverse consequences by increasing the TLR4 expression. Therefore, the useful analgesic properties of methadone should be separated from the unwanted TLR4-mediated side effects. © 2022 Advanced Biomedical Research.

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Shaghayegh Haghjooy Javanmard (8)

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Style	Citing Format
MLA	Vaseghi G, et al.. "Effects of Methadone on the Toll-Like Receptor 4 Expression in Human Non-Small Cell Lung Carcinoma A549 Cell Line Using In-Silico and in Vitro Techniques." Advanced Biomedical Research, vol. 11, no. 1, 2022, pp. -.
APA	Vaseghi G, Rashidi N, Zare N, Ghasemi F, Pourhadi M, Rafiee L, Javanmard SH (2022). Effects of Methadone on the Toll-Like Receptor 4 Expression in Human Non-Small Cell Lung Carcinoma A549 Cell Line Using In-Silico and in Vitro Techniques. Advanced Biomedical Research, 11(1), -.
Chicago	Vaseghi G, Rashidi N, Zare N, Ghasemi F, Pourhadi M, Rafiee L, Javanmard SH. "Effects of Methadone on the Toll-Like Receptor 4 Expression in Human Non-Small Cell Lung Carcinoma A549 Cell Line Using In-Silico and in Vitro Techniques." Advanced Biomedical Research 11, no. 1 (2022): -.
Harvard	Vaseghi G et al. (2022) 'Effects of Methadone on the Toll-Like Receptor 4 Expression in Human Non-Small Cell Lung Carcinoma A549 Cell Line Using In-Silico and in Vitro Techniques', Advanced Biomedical Research, 11(1), pp. -.
Vancouver	Vaseghi G, Rashidi N, Zare N, Ghasemi F, Pourhadi M, Rafiee L, et al.. Effects of Methadone on the Toll-Like Receptor 4 Expression in Human Non-Small Cell Lung Carcinoma A549 Cell Line Using In-Silico and in Vitro Techniques. Advanced Biomedical Research. 2022;11(1):-.
BibTex	@article{ author = {Vaseghi G and Rashidi N and Zare N and Ghasemi F and Pourhadi M and Rafiee L and Javanmard SH}, title = {Effects of Methadone on the Toll-Like Receptor 4 Expression in Human Non-Small Cell Lung Carcinoma A549 Cell Line Using In-Silico and in Vitro Techniques}, journal = {Advanced Biomedical Research}, volume = {11}, number = {1}, pages = {-}, year = {2022} }
RIS	TY - JOUR AU - Vaseghi G AU - Rashidi N AU - Zare N AU - Ghasemi F AU - Pourhadi M AU - Rafiee L AU - Javanmard SH TI - Effects of Methadone on the Toll-Like Receptor 4 Expression in Human Non-Small Cell Lung Carcinoma A549 Cell Line Using In-Silico and in Vitro Techniques JO - Advanced Biomedical Research VL - 11 IS - 1 SP - EP - PY - 2022 ER -

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