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Identification of an Immunoglobulin M (Igm) Antibody Against Enolase 1 Protein (Eno1) Derived From Hek-293 Cells in Patients With Kidney Failure Publisher



Jazayeri MH1, 2 ; Sadri M2 ; Mostafaie A3 ; Nedaeinia R4
Authors
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Authors Affiliations
  1. 1. Immunology Research Center, Iran University of Medical Sciences, Shahid Hemmat Highway, P.O Box: 14665-354, Tehran, 1449614535, Iran
  2. 2. Department of Immunology, Faculty of Medical Sciences, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
  4. 4. Applied Physiology Research Center, Cardiovascular Research Institute, Isfahan University of Medical Science, Isfahan, Iran

Source: International Journal of Peptide Research and Therapeutics Published:2020


Abstract

Abstract: Kidney failure is one of the most important challenges in medicine. In this study, we used HEK-293 kidney cells to evaluate and compare changes in the expression of natural antibodies in patients with kidney failure and healthy controls. We analyzed the immunoreactivity of two groups of pooled sera from patient and control groups against antigens derived from HEK-293 cell lysates. The cell lysates were immunoblotted with the pooled sera from the groups. The results showed that the patients’ sera contained IgM antibodies, which recognized enolase 1 proteins. Graphic Abstract: [Figure not available: see fulltext.] © 2019, Springer Nature B.V.
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