Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Preparation and in Vitro Evaluation of Inhalation Powder Containing Fluconazole Nanoparticles for the Treatment of Fungal Lung Infections Publisher



Taymouri S1 ; Ansari H1 ; Shafiee F2 ; Varshosaz J1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Pharmaceutics, School of Pharmacy, Novel Drug Delivery Systems Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Isfahan Medical School Published:2023


Abstract

Background: Immunocompromised persons are very susceptible to fungal infections. It is desirable to treat pulmonary fungal infections with specific delivery of drugs to the lungs. Methods: Zein nanoparticles containing fluconazole were prepared by anti-solvent method. Their physicochemical properties were investigated. From the optimal formulation, dry powder was prepared with lactose or mannitol using spray drying and aerosolization characteristics of the dry powders were evaluated using Anderson's cascade impactor. Antifungal effect was investigated against candida albicans and minimum inhibitory concentration was determined. Cytotoxicity of free fluconazole, dry powder containing drug loaded nanoparticles, dry powder containing nanoparticles without drug was also evaluated against A549 cell line by MTT method. Findings: The size of nanoparticles was in the range of 88.70 ± 1.35 to 226.90 ± 5.40 nm, and the percentage of loading efficiency was in the range of 57.58 ± 3.61 to 92.94 ± 0.34. In the prepared dry powder samples, all the powder samples had a high emitted dose percentage. Cytotoxicity test showed that in general none of the groups had significant cytotoxic effects even at the highest studied concentration. Conclusion: Using lactose as carrier, can improve the aerodynamic properties of dry powder compared with mannitol. The prepared inhaled fluconazole powder has a suitable fine particle fraction % and a low median mass aerodynamic diameter and can be well deposited in the lung. In addition, powder containing fluconazole was safe and its effect was comparable with fluconazole. © 2023 Isfahan University of Medical Sciences(IUMS). All rights reserved.
Related Docs
View other Related Docs
1. Investigating the Performance of Drug Delivery System of Fluconazole Made of Nano–Micro Fibers Coated on Cotton/Polyester Fabric, Journal of Materials Science: Materials in Medicine (2017)
2. Comparison of the Antifungal Activity of Fluconazole- and Ketoconazole-Loaded Pcl/Pvp Nanofibrous Mat, Bulletin of Materials Science (2021)
3. Evaluation of the Therapeutic Effect of 15 Ppm Silver Nanoparticle Spray Compared to Clotrimazole 1% on Candida Vaginitis: A Randomized Controlled Clinical Trial, Journal of Family and Reproductive Health (2023)
4. Evaluation of Rapid Diagnosis of Candidemia by Agglutination Test Using Gold Nanoparticles, Journal of Isfahan Medical School (2022)
5. Formulation and Physicochemical Characterization of Azithromycin-Loaded Cubosomes, Research in Pharmaceutical Sciences (2023)
6. Evaluation and Comparison of Antifungal Effect of Voriconazole With Nystatin on Candida Species Derived From Neoplastic Patients Undergoing Maxillofacial Radiotherapy, Dental Research Journal (2023)
7. Release Studies on Ciprofloxacin Loaded Non-Ionic Surfactant Vesicles, Avicenna Journal of Medical Biotechnology (2015)
8. Synergistic Activity Between Echinophora Platyloba Dc Ethanolic Extract and Azole Drugs Against Clinical Isolates of Candida Albicans From Women Suffering Chronic Recurrent Vaginitis, Journal de Mycologie Medicale (2014)
Experts (# of related papers)
View all Related Experts
Somayeh Taymouri (14)
Jaleh Varshosaz (13)
Other Related Docs
9. In Vitro Antifungal Susceptibility Profile of Miltefosine Against a Collection of Azole and Echinocandins Resistant Fusarium Strains, Journal of Fungi (2022)
10. Antifungal Activity of Anethum Graveolens Extract and Atrovastatin Against Candida Species Compared to Fluconazole, Journal of Isfahan Medical School (2015)
11. Ciprofloxacin Nano-Niosomes for Targeting Intracellular Infections: An in Vitro Evaluation, Journal of Nanoparticle Research (2013)
12. Development and Evaluation of Wafer Loaded With Sertaconazole Solid Dispersion for the Treatment of Oral Candidiasis, Brazilian Journal of Pharmaceutical Sciences (2023)
13. Biodistribution of Amikacin Solid Lipid Nanoparticles After Pulmonary Delivery, BioMed Research International (2013)
14. Antifungal Effect of Atorvastatin Against Candida Species in Comparison to Fluconazole and Nystatin, Medicine and Pharmacy Reports (2019)
15. Efavirenz Oral Delivery Via Lipid Nanocapsules: Formulation, Optimisation, and Ex-Vivo Gut Permeation Study, IET Nanobiotechnology (2018)
16. Feasibility of Haloperidol-Anchored Albumin Nanoparticles Loaded With Doxorubicin As Dry Powder Inhaler for Pulmonary Delivery, Pharmaceutical Development and Technology (2015)
17. Antibacterial, Antifungal and Cytotoxic Evaluation of Some New Quinazolinone Derivatives, Research in Pharmaceutical Sciences (2012)
18. In Vitro Activity of Two Nanoparticles on Clinical Isolates of Candida Parapsilosis, Showing Resistance Against Antifungal Agents in Children, Archives of Clinical Infectious Diseases (2017)
19. Ciprofloxacin Loaded Alginate/Chitosan and Solid Lipid Nanoparticles, Preparation, and Characterization, Journal of Dispersion Science and Technology (2012)
20. In Vitro Activities of Six Antifungal Drugs Against Candida Glabrata Isolates: An Emerging Pathogen, Jundishapur Journal of Microbiology (2016)
21. Physicochemical, Pharmacodynamic and Pharmacokinetic Characterization of Soluplus Stabilized Nanosuspension of Tacrolimus, Current Drug Delivery (2017)
22. Preparing Simvastatin Nanoparticles by a Combination of Ph-Sensitive and Timed-Release Approaches for the Potential Treatment of Ulcerative Colitis, Journal of Biomaterials Applications (2022)
23. Antibacterial, Antifungal and Cytotoxic Evaluation of Some New 2,3-Disubstituted 4(3H)-Quinazolinone Derivatives, Research in Pharmaceutical Sciences (2012)
24. In Vitro Antifungal Susceptibility Profile of Iranian Fusarium Isolates: Emphasising on the Potent Inhibitory Effect of Efinaconazole Compared to Other Drugs, Mycoses (2023)
25. The Characterization and Antileishmanial Evaluation on Leishmania Major With Chitosan/Zno Bio-Nanocomposite As Drug Delivery Systems, Nanomedicine Research Journal (2022)
26. Evaluation of Thermosensitive Chitosan Hydrogel Containing Gefitinib Loaded Cellulose Acetate Butyrate Nanoparticles in a Subcutaneous Breast Cancer Model, International Journal of Pharmaceutics (2022)
27. Antifungal Activity of Almond (Prunus Amygdalus) Hull Extracts Against Clinical Isolates of Candida Albicans, Current Medical Mycology (2024)
28. Determining the Frequency of Pathogenic Yeasts in Clinical Urine Samples and Tracking the Trichosporon Using Gold Nanoparticles; [تعیین فراوانی انواع مخمرهای بیماریزا در نمونههای بالینی ادرار و ردیابی مخمر ترایکوسپورونبا استفاده از نانوذرات طلا], Journal of Isfahan Medical School (2024)
29. Fabrication and Characterization of Injectable Thermosensitive Hydrogel Containing Dipyridamole Loaded Polycaprolactone Nanoparticles for Bone Tissue Engineering, Journal of Drug Delivery Science and Technology (2021)
30. Stability and Antimicrobial Effect of Amikacin-Loaded Solid Lipid Nanoparticles, International Journal of Nanomedicine (2011)
31. Co-Delivery of Carboplatin and Doxorubicin Using Zif-8 Coated Chitosan-Poly(N-Isopropyl Acrylamide) Nanoparticles Through a Dual Ph/Thermo Responsive Strategy to Breast Cancer Cells, International Journal of Biological Macromolecules (2024)
32. Lipid Nanocapsules for Imatinib Delivery: Design, Optimization and Evaluation of Anticancer Activity Against Melanoma Cell Line, Iranian Journal of Pharmaceutical Research (2019)
33. Preparation and Physicochemical Evaluation of Nystatine Mucoadhesive Buccal Film, Journal of Reports in Pharmaceutical Sciences (2013)
34. Novel Nanoscale Vanillin Based Covalent Triazine Framework As a Novel Carrier for Sustained Release of Imatinib, Polymers for Advanced Technologies (2023)
35. Comparing the Drug Loading and Release of Silica Aerogel and Pva Nano Fibers, Journal of Non-Crystalline Solids (2019)
36. Multicenter Identification and Antifungal Susceptibility Patterns of Candida Species Isolated From Clinical Samples, Jundishapur Journal of Microbiology (2017)
37. Pulmonary Delivery of Docetaxel and Celecoxib by Plga Porous Microparticles for Their Synergistic Effects Against Lung Cancer, Anti-Cancer Agents in Medicinal Chemistry (2022)
38. In-Vivo Efficiency of the Novel Azole Compounds (Attaf-1 and Attaf-2) Against Systemic Candidiasis in a Murine Model, Journal of Medical Mycology (2023)
39. Fabrication of Polymeric Nanoparticles of Poly(Ethylene-Co-Vinyl Acetate) Coated With Chitosan for Pulmonary Delivery of Carvedilol, Journal of Applied Polymer Science (2014)
40. Formulation and Optimization of Celecoxib-Loaded Plga Nanoparticles by the Taguchi Design and Their in Vitro Cytotoxicity for Lung Cancer Therapy, Pharmaceutical Development and Technology (2015)
41. Topical Effectiveness of Different Concentrations of Nanosilver Solution on Leishmania Major Lesions in Mice (Balb/C), Journal of Isfahan Medical School (2011)
42. Preparation and Evaluation of Inhalable Dry Powder Containing Glucosamine-Conjugated Gefitinib Slns for Lung Cancer Therapy, Drug Development and Industrial Pharmacy (2020)
43. Preparation and Characterization of Spray-Dried Inhalable Powders Containing Polymeric Micelles for Pulmonary Delivery of Paclitaxel in Lung Cancer, Journal of Pharmacy and Pharmaceutical Sciences (2018)
44. Preparation and Pharmaceutical Evaluation of Miconazole Nitrate Mucoadhesive Films for Vaginal Candidiasis, Journal of Isfahan Medical School (2013)
45. Particle Size Design of Plga Microspheres for Potential Pulmonary Drug Delivery Using Response Surface Methodology, Journal of Microencapsulation (2009)
46. Investigation of Biological Effects of New Tetrazole Compounds on Cancer Cell Lines (Hela, Mcf-7) and Common Microorganisms (Staphylococcus Aureus, Escherichia Coli, Candida Albicans), Journal of Isfahan Medical School (2023)
47. Antifungal Activity of Zingiber Officinale Rosc. Essential Oil Against Fluconazole Resistant Vaginal Isolates of Candida Albicans, Journal of Medicinal Plants (2007)
48. Antifungal Susceptibility Testing of Candida Species Isolated From the Immunocompromised Patients Admitted to Ten University Hospitals in Iran: Comparison of Colonizing and Infecting Isolates, BMC Infectious Diseases (2017)
49. Preparation of Chitosan Nanoparticles for Simultaneous Drug Delivery of Dacarbazine and Enoxaparin in Melanoma, Carbohydrate Polymers (2023)
50. In Vitro Activity of Juglone (5-Hydroxy-1,4-Naphthoquinone) Against Both Fluconazole-Resistant and Susceptible Candida Isolates; [Actividad in Vitro De Juglona (5-Hidroxi-1,4-Naftoquinona) Contra Aislamientos De Candida Resistentes Y Sensibles a Fluconazol], Revista Iberoamericana de Micologia (2022)