Structural and Functional Impact of Missense Mutations in Tpmt: An Integrated Computational Approach Publisher Pubmed



Fazelnajafabadi E¹ ; Vahdat Ahar E² ; Fattahpour S¹ ; Sedghi M^{1, 3} Show Affiliations
Source: Computational Biology and Chemistry Published:2015

Abstract

Background Thiopurine S-methyltransferase (TPMT) detoxifies thiopurine drugs which are used for treatment of various diseases including inflammatory bowel disease (IBD), and hematological malignancies. Individual variation in TPMT activity results from mutations in TPMT gene. In this study, the effects of all the known missense mutations in TPMT enzyme were studied at the sequence and structural level Methods A broad set of bioinformatic tools was used to assess all the known missense mutations affecting enzyme activity. The effects of these mutations on protein stability, aggregation propensity, and residue interaction network were analyzed. Results Our results indicate that the missense mutations have diverse effects on TPMT structure and function. Stability and aggregation propensities are affected by various mutations. Several mutations also affect residues in ligand binding site. Conclusions In vitro study of missense mutation is laborious and time-consuming. However, computational methods can be used to obtain information about effects of missense mutations on protein structure. In this study, the effects of most of the mutations on enzyme activity could be explained by computational methods. Thus, the present approach can be used for understanding the protein structure-function relationships. © 2015 Elsevier Ltd. All rights reserved.