An Efficient Deep Eutectic Magnetic Nano Gel for Rapid Ultrasound-Assisted Dispersive Μ-Solid Phase Extraction of Residue of Tetracyclines in Food Samples Publisher



Shirani M¹ ; Faraji M² ; Rashidi Nodeh H³ ; Akbariadergani B⁴ ; Sepahi S⁵ Show Affiliations
Source: Journal of Food Science and Technology Published:2023

Abstract

In the present study, a magnetic nano gel as the sorbent which is the combination of octatonic acid: cumarin as eutectic solvent and Fe3O4@SiO2 was introduced as the sorbent in ultrasound-assisted dispersive µ-solid phase extraction process coupled with high performance liquid chromatography with photo diode array detector for simultaneous separation and determination of tetracyclines residues in food samples. FT-IR, SEM, VSM were used for the characterization of the synthetized magnetic nano gel. Under obtained optimum conditions, the obtained linear ranges were 1.5–500 (µg L−1), 2.5–750 (µg L−1), 2–750 (µg L−1), and 2.5–500 (µg L−1) for tetracycline, oxytetracycline, chlortetracycline, and doxycycline, respectively. Moreover, the below level of quantification (BLQ) (based on S/N = 3) of 0.47 µg L−1, 0.11 µg L−1, 0.85 µg L−1, 0.66 µg L−1, 0.81 µg L−1 and the limit of quantification (based on S/N = 10) of 1.61, 2.74, 2.23 (µg L−1), and 2.66 were achieved for tetracycline, oxytetracycline, chlortetracycline, and doxycycline, respectively. The intra-day and inter-day precision (%) of the procedure were less than 3.2 and 3.8, respectively. The recoveries over 95% confirmed high sufficiency of the proposed method for application in complex matrices such as honey, milk, and egg. Graphical abstract: [Figure not available: see fulltext.]. © 2023, Association of Food Scientists & Technologists (India).