Comparison of Different Methods for Erythroid Differentiation in the K562 Cell Line Publisher Pubmed



Shariati L¹ ; Modaress M² ; Khanahmad H^{2, 3} ; Hejazi Z² ; Tabatabaiefar MA^{2, 3} ; Salehi M² ; Modarressi MH^{1, 4}
Source: Biotechnology Letters Published:2016

Abstract

Objective: To compare methods for erythroid differentiation of K562 cells that will be promising in the treatment of beta-thalassemia by inducing γ-globin synthesis. Results: Cells were treated separately with: RPMI 1640 medium without glutamine, RPMI 1640 medium without glutamine supplemented with 1 mM sodium butyrate, RPMI 1640 medium supplemented with 1 mM sodium butyrate, 25 µg cisplatin/ml, 0.1 µg cytosine arabinoside/ml. The highest differentiation (84 %) with minimum toxicity was obtained with cisplatin at 15 µg /ml. Real-time RT-PCR showed that expression of the γ-globin gene was significantly higher in the cells differentiated with cisplatin compared to undifferentiated cells (P < 0.001). Conclusions: Cisplatin is useful in the experimental therapy of ß-globin gene defects and can be considered for examining the basic mechanism of γ-reactivation. © 2016, Springer Science+Business Media Dordrecht.