Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Effects of Chitosan As a Novel Obturation Material for Pulpectomized Teeth on Periapical Inflammation, Periodontal Ligament Widening, and Hard Tissue Resorption: A Preliminary Exploratory Study on Dogs Publisher



Basir L1 ; Babashahi E1 ; Razavi SM2 ; Manshaei M3 ; Rakhshan V4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Pediatric Dentistry, School of Dentistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
  2. 2. Department of Oral and Maxillofacial Pathology, Dental School, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Dental Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Tehran, Iran

Source: Frontiers in Dentistry Published:2024


Abstract

Objectives: This preliminary animal study was conducted to assess the effects of chitosan as a novel obturation material for pulpectomized teeth on periapical inflammation, periodontal ligament (PDL) widening, and hard tissue resorption. Materials and Methods: Forty premolar root canals in two mature dogs were obturated with zinc oxide eugenol (ZOE) and an experimental 3% chitosan paste (n=20 in each group). The teeth were then restored with amalgam. After 28 days, the dogs were sacrificed, and histopathological assessment was performed. The amount of resorbed obturation material, degree of inflammatory response, degree of PDL widening, and the number of bone/cementum/dentin resorption defects were recorded under ×40 and ×200 magnifications. Data were analyzed using the Mann-Whitney U test, one-sample Wilcoxon signed-rank test, and Fisher’s exact test (α=0.05). Results: Bone, cementum, and dentin resorption were seen in 6, 10, and 1 chitosan-obturated canals and 14, 15, and 0 ZOE-obturated canals, respectively. Only the bone resorption defects were significantly fewer in the chitosan group (P=0.026). Mild, moderate, and severe inflammation were observed in 17, 3, and 0 chitosan-obturated canals, and 7, 9, and 4 ZOE-filled canals, respectively (P=0.004). Mild, moderate, and severe PDL widening were seen around 15, 5, and 0 chitosan-filled canals and 7, 12, and 1 ZOE-filled canals, respectively (P=0.025). Conclusion: The 3% chitosan was superior to ZOE in terms of causing less inflammation and PDL widening. It also decreased bone resorption and acted similar to ZOE in terms of dentin and cementum resorption. © 2024 The Authors. Published by Tehran University of Medical Sciences.
Related Docs
View other Related Docs
1. Hard Tissue Formation in Pulpotomized Primary Teeth in Dogs With Nanomaterials Mcm-48 and Mcm-48/Hydroxyapatite: An in Vivo Animal Study, BMC Oral Health (2024)
2. Pulp Response Following Direct Pulp Capping With Dentin Adhesives and Mineral Trioxide Aggregate; an Animal Study, Iranian Endodontic Journal (2017)
3. Histological Evaluation of Proroot Mineral Trioxide Aggregate and Cold Ceramic As Root-End Filling Materials in Animal Models, Dental Research Journal (2023)
4. Periodontal Healing of Replanted Dog Teeth Stored in Milk and Egg Albumen, Dental Traumatology (2008)
5. Effectiveness of Buccal Pouch Grafting in Minimizing Loss of Alveolar Dimension: A Canine Investigation, Dental Research Journal (2019)
6. Histologic Evaluation of Apical Pulp of Immature Apex Following Extraction, Surface Treatment, and Replantation in Different Storage Media in Dogs, Dental Traumatology (2015)
7. The Accuracy of Three Apex Locators in Determining the Location of Strip Root Perforation in Different Environments, Iranian Endodontic Journal (2021)
8. Outcomes of Revascularization Treatment in Immature Dog's Teeth, Dental Traumatology (2014)
Experts (# of related papers)
View all Related Experts
Abbasali Khademi (24)
Sayed Mohammad Razavi (19)
Other Related Docs
9. Prevalence of Middle Mesial Root Canal in Mandibular Molars in an Iranian Population: A Micro-Computed Tomography Study, Dental Research Journal (2024)
10. Combined Effects of Calcium Hydroxide and Photobiomodulation Therapy on Apexogenesis of Immature Permanent Teeth in Dogs, Journal of Photochemistry and Photobiology B: Biology (2020)
11. Mechanical or Cold Lateral Compaction: The Incidence of Dentinal Defects, Dental Research Journal (2015)
12. Mineral Trioxide Aggregate Obturation Quality With Two Obturation Techniques in Severe Curved Root Canals – a Micro-Ct Study, BMC Oral Health (2024)
13. Influence of Blood on the Accuracy of Raypex 5 and Root Zx Electronic Foramen Locators: An in Vivo Study, Brazilian Dental Journal (2016)
14. Effect of Cpp-Acp Application on Flexural Strength of Bleached Enamel and Dentin Complex, Operative Dentistry (2011)
15. Comparative Evaluation of Microtensile Bond Strength of Four Glass-Containing Materials With Primary Teeth Dentin, Iranian Journal of Pediatrics (2019)
16. Effect of Surface Treatments on Fracture Resistance of Root Filled Teeth With Bonded Fibre Posts, Dental Traumatology (2014)
17. A Comparative Study of Three Treatment Methods of Direct Pulp Capping in Canine Teeth of Cats: A Histologic Evaluation, Lasers in Medical Science (2010)
18. Comparative Evaluation of the Effects of Co2 and Er:Yag Lasers on Smear Layer Removal and Blood Cell Attachment to Tooth Root Surfaces, Journal of Lasers in Medical Sciences (2020)
19. Antibacterial Effect of Nano-Chlorhexidine on Enterococcus Faecalis Biofilm in Root Canal System:An in Vitro Study, Dental Research Journal (2022)
20. In Vitro Evaluation of Dentin Tubule Occlusion by Three Bioactive Materials: A Scanning Electron Microscopic Study, Dental Research Journal (2019)
21. The Effects of Simvastatin on Bone Remodeling, Tooth Movement and Root Resorption in Orthodontic Treatments, Biomedical and Pharmacology Journal (2013)
22. Marginal Leakage of Class V Composite Resin Restorations, Dental Hypotheses (2018)
23. Enhancing Adhesive Performance With N, N, N', N'-Tetrakis (2-Pyridyl Methyl) Ethylenediamine Matrix Metalloproteinase Inhibitors: A Comprehensive Study of Degree of Conversion, Microleakage, and Micro-Tensile Bond Strength in Dental Adhesives, Journal of Conservative Dentistry and Endodontics (2025)
24. Immediate and Delayed Post Space Preparations in Endodontically Treated Teeth: A Scoping Review, BMC Oral Health (2022)
25. Comparative Effectiveness of Hand and Ultrasonic Instrumentations in Root Surface Planing in Vitro, Journal of Clinical Periodontology (2004)
26. Radiographic Quality of Root Canal Obturation Performed by Fifth Year Students of Hamadan Dental School, Iranian Endodontic Journal (2017)
27. Influence of Tooth Length on the Accuracy of the Root Zx Electronic Apical Foramen Locator: An Ex Vivo Study, Acta Odontologica Scandinavica (2014)
28. The Effect of Time and Storage Environment on Dimensional Changes of Acrylic Resin Post Patterns, Open Dentistry Journal (2015)
29. Investigating the Effect of Nonrigid Connectors on the Success of Tooth-And-Implant-Supported Fixed Partial Prostheses in Maxillary Anterior Region: A Finite Element Analysis (Fea), International Journal of Dentistry (2021)
30. Tissue Engineering: Dentin – Pulp Complex Regeneration Approaches (A Review), Tissue and Cell (2017)
31. Clinical and Radiographic Success of Theracal Versus Formocresol in Primary Teeth Pulpotomy: A Systematic Review and Meta-Analysis, Saudi Dental Journal (2024)
32. Effects of Propolis-Based Herbal Toothpaste on Dentine Hypersensitivity, International Dental Journal (2024)
33. Canine Impaction and Fenestration Technique, Innovative Perspectives in Oral and Maxillofacial Surgery (2021)
34. Retro Mta and Tricalcium Phosphate/Retro Mta for Guided Tissue Regeneration of Periodontal Dehiscence Defects in a Dog Model: A Pilot Study, Biomaterials Research (2019)
35. Apical Microleakage Assessment of Calcium Silicate-Based and Resin-Based Sealers by Fluid Filtration Technique, Journal of Dental Materials and Techniques (2022)
36. An Ex Vivo Analysis of Apical Transportation in Root Canals Prepared With Bt Race Rotary and Hand K-Flexofile by Cone Beam Computed Tomography, Journal of Mazandaran University of Medical Sciences (2020)
37. Rotary Versus Manual Instrumentation for Root Canal Preparation in Primary Teeth: A Systematic Review and Meta-Analysis of Clinical Trials, Contemporary Clinical Dentistry (2022)
38. Comparison of Cone Beam Computed Tomography and Digital Radiography in Detecting Separated Endodontic Files and Strip Perforation, Applied Sciences (Switzerland) (2020)
39. Comparison of Clinical and Radiographic Success Between Mta and Biodentine in Pulpotomy of Primary Mandibular Second Molars With Irreversible Pulpitis: A Randomized Double-Blind Clinical Trial, International Journal of Dentistry (2022)
40. Evaluation of the Apical Microleakage of Mta Fillapex, Ah26, and Endofill Sealers; [Avaliacao Da Microinfiltracao Apical De Cimentos Mta Fillapex Ah26 E Endofill], Brazilian Dental Science (2020)
41. Comparative Evaluation of Hydroxyapatite and Nano-Bioglass in Two Forms of Conventional Micro-And Nano-Particles in Repairing Bone Defects (An Animal Study), Indian Journal of Dental Research (2015)
42. The Efficacy Comparison of Ibuprofen, Acetaminophen-Codeine, and Placebo Premedication Therapy on the Depth of Anesthesia During Treatment of Inflamed Teeth, Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology (2006)
43. A New Storage Medium for an Avulsed Tooth, Journal of Contemporary Dental Practice (2008)
44. The Effect of Micro‑Osteoperforation on Root Resorption,Pulp Vitality, and Biological Changes of Teeth Subjected to Orthodontic Tooth Movement:A Systematic Review Study, Dental Research Journal (2023)
45. Influence of Intracanal Irrigants on Coronal Fracture Resistance of Endodontically Treated and Bleached Teeth: An in Vitro Study, Contemporary Clinical Dentistry (2017)
46. Human Pulp Response to Direct Pulp Capping and Miniature Pulpotomy With Mta After Application of Topical Dexamethasone: A Randomized Clinical Trial, Iranian Endodontic Journal (2016)
47. Effect of Different Protective Bases on Ph Changes and Hydrogen Peroxide Microleakage During Intracoronal Bleaching, Frontiers in Dentistry (2023)
48. Effect of Polyhydroxybutyrate/Chitosan/Bioglass Nanofiber Scaffold on Proliferation and Differentiation of Stem Cells From Human Exfoliated Deciduous Teeth Into Odontoblast-Like Cells, Materials Science and Engineering C (2018)
49. A Randomized Controlled Clinical Trial Comparing Clinical and Radiographic Success Rates of Mta and Rhbmp2 in Pulpotomy of Primary Teeth, Frontiers in Dentistry (2024)
50. The Effect of Intraorifice Barriers (Theracal Lc, Lime-Lite and Ionoseal) on the Fracture Resistance and Failure Patterns of Endodontically Treated Teeth Submitted to Intracoronal Bleaching, Dental Research Journal (2023)