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The Study of Antibacterial Effect of Humulus Lupulus on Some of Gram Positive & Gram Negative Bacteria



Kasra Kermanshahi R1 ; Nasr Esfahani B2 ; Esmi Serkani J3 ; Asghari GHR4 ; Pour Babaie AA3
Authors
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Authors Affiliations
  1. 1. Department of Microbiology, School of Sciences, Alzahra University, Tehran, Iran
  2. 2. Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Microbiology, Islamic Azad University of Qom, Qom, Iran
  4. 4. Department of Pharmacognosy, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Medicinal Plants Published:2009

Abstract

Background: In recent years, a new view has been stablished toward pharmaceutical plants and applicational use of them all over the world and researchers, in various medical courses are investigating about this issue. Objective: On this basis, antibacterial effect of Humulus lupulus, the pharmaceutical plant which has an important place in traditional medicine of Iran, was evaluated on some of bacteria. Methods: Based on scientific methods after being dried, prepared extract by using macerasion method. Antibacterial effects of ethanol extract were studied on Staphylococcus aureus (1112PTCC), Escherichia coli (1276PTCC), Bacillus subtilis (1023PTCC), Psedomonas aeroginosa (1430PTCC), by using spreading method, well plate method and MIC. Results: The ethanol extract of Humulus lupulus showed antimicrobial activity on S. aureus and B. subtilis. The extract exhibited antimicrobial activity at a range of 125 mg/ml to 62/5 mg/ml against S. aureus and B. subtilis respectively. Conclusion: With the increase of worldwide antibiotic resistance of many strains of disease producing bacteria, there is a need to access new and complementary approaches to antibiotic therapy. Extract of Humulus lupulus has antibacterial effect. The antimicrobial activity of Humulus lupulus against a variety of gram positive bacteria confirm its use for treatment of infectious.
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