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Functional Analyses of Recombinant Mouse Hepcidin-1 in Cell Culture and Animal Model Publisher Pubmed



Yazdani Y1 ; Keyhanvar N2 ; Kalhor HR3 ; Rezaei A4
Authors

Source: Biotechnology Letters Published:2013


Abstract

Hepcidin is a peptide hormone that plays an important role in iron metabolism. We have produced a recombinant mouse hepcidin-1 by using baculovirus expression system. Its expression yield was 25 μg/ml when cell culture media were supplemented with a protease inhibitor cocktail. The recombinant mouse hepcidin-1 and synthetic human hepcidin-25 had similar effects on reducing ferroportin expression in J774A cell line and in peritoneal macrophages. However, synthetic human hepcidin-25 was more efficient than recombinant mouse hepcidin-1 in reducing iron concentration in blood circulation (p < 0.01). © 2013 Springer Science+Business Media Dordrecht.
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