Functional Analyses of Recombinant Mouse Hepcidin-1 in Cell Culture and Animal Model

Functional Analyses of Recombinant Mouse Hepcidin-1 in Cell Culture and Animal Model Publisher Pubmed

Yazdani Y¹ ; Keyhanvar N² ; Kalhor HR³ ; Rezaei A⁴

Show Affiliations

1. Infectious Diseases Research Center and Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran
2. Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran
3. Biochemistry Research Laboratory, Department of Chemistry, Sharif University of Technology, Tehran, Iran
4. Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Biotechnology Letters Published:2013

Abstract

Hepcidin is a peptide hormone that plays an important role in iron metabolism. We have produced a recombinant mouse hepcidin-1 by using baculovirus expression system. Its expression yield was 25 μg/ml when cell culture media were supplemented with a protease inhibitor cocktail. The recombinant mouse hepcidin-1 and synthetic human hepcidin-25 had similar effects on reducing ferroportin expression in J774A cell line and in peritoneal macrophages. However, synthetic human hepcidin-25 was more efficient than recombinant mouse hepcidin-1 in reducing iron concentration in blood circulation (p < 0.01). © 2013 Springer Science+Business Media Dordrecht.

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Mehdi Nematbakhsh (2)

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Style	Citing Format
MLA	Yazdani Y, et al.. "Functional Analyses of Recombinant Mouse Hepcidin-1 in Cell Culture and Animal Model." Biotechnology Letters, vol. 35, no. 8, 2013, pp. 1191-1197.
APA	Yazdani Y, Keyhanvar N, Kalhor HR, Rezaei A (2013). Functional Analyses of Recombinant Mouse Hepcidin-1 in Cell Culture and Animal Model. Biotechnology Letters, 35(8), 1191-1197.
Chicago	Yazdani Y, Keyhanvar N, Kalhor HR, Rezaei A. "Functional Analyses of Recombinant Mouse Hepcidin-1 in Cell Culture and Animal Model." Biotechnology Letters 35, no. 8 (2013): 1191-1197.
Harvard	Yazdani Y et al. (2013) 'Functional Analyses of Recombinant Mouse Hepcidin-1 in Cell Culture and Animal Model', Biotechnology Letters, 35(8), pp. 1191-1197.
Vancouver	Yazdani Y, Keyhanvar N, Kalhor HR, Rezaei A. Functional Analyses of Recombinant Mouse Hepcidin-1 in Cell Culture and Animal Model. Biotechnology Letters. 2013;35(8):1191-1197.
BibTex	@article{ author = {Yazdani Y and Keyhanvar N and Kalhor HR and Rezaei A}, title = {Functional Analyses of Recombinant Mouse Hepcidin-1 in Cell Culture and Animal Model}, journal = {Biotechnology Letters}, volume = {35}, number = {8}, pages = {1191-1197}, year = {2013} }
RIS	TY - JOUR AU - Yazdani Y AU - Keyhanvar N AU - Kalhor HR AU - Rezaei A TI - Functional Analyses of Recombinant Mouse Hepcidin-1 in Cell Culture and Animal Model JO - Biotechnology Letters VL - 35 IS - 8 SP - 1191 EP - 1197 PY - 2013 ER -

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