Isolation and Identification of Nocardia Species From Soil Samples According to Comparison of Three Methods

Isolation and Identification of Nocardia Species From Soil Samples According to Comparison of Three Methods

Faghri J^{1, 4} ; Bourbour S^{1, 4} ; Moghim S^{1, 4} ; Safaei HG^{1, 4} ; Esfahani BN^{1, 4} ; Meidani M² ; Akbari M³ ; Hosseini NS^{1, 4}

Show Affiliations

1. Department of Microbiology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2. Department of Infectious and Tropical Diseases, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3. Department of Epidemiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4. Department of Microbiology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Isfahan Medical School Published:2013

Abstract

Background: Aerobic Actinomycetes, especially Nocardia are of the most common pathogens of systemic infections in worldwide. It is seen from skin infections caused by trauma in normal-immune system host to severe lung diseases or diseases of the central nervous system in patients with immune deficiency. Nocardia species (spp.) are soil-indwelling bacteria. This study aimed to compare three phenotypic methods for isolation and identification of Nocardia spp. Methods: In this study, 60 soil samples were collected from areas of agricultural lands, gardens and around some of the hospitals with different soil temperature and pH in Isfahan, Iran. In order to isolation of Nocardia spp. from soil, three different methods were studied: Paraffin baiting method (McClung's carbon free broth with paraffin bait), Paraffin coated slides, and Slip-burid method. Findings: Paraffin baiting method was surveyed on 10 soil samples and no results were gained. Paraffin coated slides was surveyed on 4 soil samples (same soil samples) but no results were attained. So, slip-burid method of isolation was tested. In this method, 15 out of 60 soil samples (25%) were positive for Nocardia spp. and colonies were suspected to Nocardia spp. Conclusion: In this study, slip-burid method found to be more effective for Nocardia spp. Isolation.

2. Molecular Analysis and Species Diversity of Nocardia in the Hospital Environment in a Developing Country, a Potential Health Hazard, Journal of Medical Microbiology (2017)

3. Biodegradation of Polycyclic Aromatic Hydrocarbons, Phenol and Sodium Sulfate by Nocardia Species Isolated and Characterized From Iranian Ecosystems, Scientific Reports (2020)

4. First Report of the Isolation of Nocardia Thailandica From the Bronchoalveolar Lavage of a Patient in Iran, Iranian Journal of Medical Sciences (2018)

5. Assessment of Indoor and Outdoor Airborne Fungi in an Educational, Research and Treatment Center, Italian Journal of Medicine (2017)

6. First Case Report of Pulmonary and Cutaneous Nocardiosis Caused by Nocardia Mexicana in Iran, Access Microbiology (2019)

7. Comment on a Primary Cutaneous Nocardiosis of the Hand, Open Access Macedonian Journal of Medical Sciences (2018)

8. Clinical Isolation of Nocardia Cyriacigeorgica From Patients With Various Clinical Manifestations, the First Report From Iran, Medical Mycology Journal (2011)

Experts (# of related papers)

Hasan Shojaei (4)

Jamshid Faghri (1)

Style	Citing Format
MLA	Faghri J, et al.. "Isolation and Identification of Nocardia Species From Soil Samples According to Comparison of Three Methods." Journal of Isfahan Medical School, vol. 31, no. 230, 2013, pp. -.
APA	Faghri J, Bourbour S, Moghim S, Safaei HG, Esfahani BN, Meidani M, Akbari M, Hosseini NS (2013). Isolation and Identification of Nocardia Species From Soil Samples According to Comparison of Three Methods. Journal of Isfahan Medical School, 31(230), -.
Chicago	Faghri J, Bourbour S, Moghim S, Safaei HG, Esfahani BN, Meidani M, Akbari M, Hosseini NS. "Isolation and Identification of Nocardia Species From Soil Samples According to Comparison of Three Methods." Journal of Isfahan Medical School 31, no. 230 (2013): -.
Harvard	Faghri J et al. (2013) 'Isolation and Identification of Nocardia Species From Soil Samples According to Comparison of Three Methods', Journal of Isfahan Medical School, 31(230), pp. -.
Vancouver	Faghri J, Bourbour S, Moghim S, Safaei HG, Esfahani BN, Meidani M, et al.. Isolation and Identification of Nocardia Species From Soil Samples According to Comparison of Three Methods. Journal of Isfahan Medical School. 2013;31(230):-.
BibTex	@article{ author = {Faghri J and Bourbour S and Moghim S and Safaei HG and Esfahani BN and Meidani M and Akbari M and Hosseini NS}, title = {Isolation and Identification of Nocardia Species From Soil Samples According to Comparison of Three Methods}, journal = {Journal of Isfahan Medical School}, volume = {31}, number = {230}, pages = {-}, year = {2013} }
RIS	TY - JOUR AU - Faghri J AU - Bourbour S AU - Moghim S AU - Safaei HG AU - Esfahani BN AU - Meidani M AU - Akbari M AU - Hosseini NS TI - Isolation and Identification of Nocardia Species From Soil Samples According to Comparison of Three Methods JO - Journal of Isfahan Medical School VL - 31 IS - 230 SP - EP - PY - 2013 ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract