For Staining of Alk Protein, the Novel D5f3 Antibody Demonstrates Superior Overall Performance in Terms of Intensity and Extent of Staining in Comparison to the Currently Used Alk1 Antibody

For Staining of Alk Protein, the Novel D5f3 Antibody Demonstrates Superior Overall Performance in Terms of Intensity and Extent of Staining in Comparison to the Currently Used Alk1 Antibody Publisher Pubmed

Taheri D^{1, 2} ; Zahavi DJ¹ ; Del Carmen Rodriguez M¹ ; Meliti A¹ ; Rezaee N³ ; Yonescu R¹ ; Ricardo BFP¹ ; Dolatkhah S² ; Ning Y¹ ; Bishop JA¹ ; Netto GJ¹ ; Sharma R¹

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1. Department of Pathology, The Johns Hopkins Hospital, 600 North Wolfe Street, Baltimore, 21287, MD, United States
2. Department of Pathology, Isfahan Kidney Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
3. Department of Surgery, Johns Hopkins Medical Institution, Baltimore, MD, United States

Source: Virchows Archiv Published:2016

Abstract

Inflammatory myofibroblastic tumor (IMT) is a rare neoplasm. Approximately 50 % of IMTs show an anaplastic lymphoma kinase (ALK) gene fusion resulting in ALK overexpression on immunohistochemistry (IHC). A novel anti-ALK monoclonal antibody (D5F3) has been suggested to be of superior sensitivity to the ALK1 antibody which is currently used. We compared the performance of D5F3 in detecting ALK protein expression in IMTs from various anatomic sites compared to the currently utilized ALK1. We selected 25 IMTs from our surgical pathology files (2005–2015). The novel rabbit monoclonal anti-human CD246 (clone D5F3) and the currently used mouse monoclonal anti-human CD246 (clone ALK1) were used for immunohistochemical staining (IHC) in an automated slide stainer. The percentage of immunoreactive tumor cells (0, <5 %, 5–50 %, >50 %) and cytoplasmic staining intensity (graded 0–3) were assessed and compared between the two antibodies. Fluorescence in situ hybridization (FISH) studies for ALK gene rearrangement were performed on 11 tumors. D5F3 antibody stained 76 % and ALK1 antibody stained 72 % of IMTs (p = 0.747). Compared to staining with ALK1, D5F3 stained a higher proportion of cases extensively (>50 % cells) (76 vs. 28 %, p < 0.001) and with high intensity (grade 3 76 % vs 0; p < 0.001). FISH and IHC findings (for both antibodies) were concordant in 9/10 (90 %) IMTs, in which results were informative. The novel anti-ALK rabbit monoclonal antibody (D5F3 clone) demonstrates superior overall performance in term of intensity and extent of staining of ALK protein in IMT. We found IHC staining with both antibody clones to correlate equally well with FISH results for detection of ALK rearrangement. © 2016, Springer-Verlag Berlin Heidelberg.

Related Docs

1. Inflammatory Myofibroblastic Tumor: Report of a Rare Case in Kidney, Iranian Journal of Kidney Diseases (2014)

Style	Citing Format
MLA	Taheri D, et al.. "For Staining of Alk Protein, the Novel D5f3 Antibody Demonstrates Superior Overall Performance in Terms of Intensity and Extent of Staining in Comparison to the Currently Used Alk1 Antibody." Virchows Archiv, vol. 469, no. 3, 2016, pp. 345-350.
APA	Taheri D, Zahavi DJ, Del Carmen Rodriguez M, Meliti A, Rezaee N, Yonescu R, Ricardo BFP, Dolatkhah S, Ning Y, Bishop JA, Netto GJ, Sharma R (2016). For Staining of Alk Protein, the Novel D5f3 Antibody Demonstrates Superior Overall Performance in Terms of Intensity and Extent of Staining in Comparison to the Currently Used Alk1 Antibody. Virchows Archiv, 469(3), 345-350.
Chicago	Taheri D, Zahavi DJ, Del Carmen Rodriguez M, Meliti A, Rezaee N, Yonescu R, Ricardo BFP, et al.. "For Staining of Alk Protein, the Novel D5f3 Antibody Demonstrates Superior Overall Performance in Terms of Intensity and Extent of Staining in Comparison to the Currently Used Alk1 Antibody." Virchows Archiv 469, no. 3 (2016): 345-350.
Harvard	Taheri D et al. (2016) 'For Staining of Alk Protein, the Novel D5f3 Antibody Demonstrates Superior Overall Performance in Terms of Intensity and Extent of Staining in Comparison to the Currently Used Alk1 Antibody', Virchows Archiv, 469(3), pp. 345-350.
Vancouver	Taheri D, Zahavi DJ, Del Carmen Rodriguez M, Meliti A, Rezaee N, Yonescu R, et al.. For Staining of Alk Protein, the Novel D5f3 Antibody Demonstrates Superior Overall Performance in Terms of Intensity and Extent of Staining in Comparison to the Currently Used Alk1 Antibody. Virchows Archiv. 2016;469(3):345-350.
BibTex	@article{ author = {Taheri D and Zahavi DJ and Del Carmen Rodriguez M and Meliti A and Rezaee N and Yonescu R and Ricardo BFP and Dolatkhah S and Ning Y and Bishop JA and Netto GJ and Sharma R}, title = {For Staining of Alk Protein, the Novel D5f3 Antibody Demonstrates Superior Overall Performance in Terms of Intensity and Extent of Staining in Comparison to the Currently Used Alk1 Antibody}, journal = {Virchows Archiv}, volume = {469}, number = {3}, pages = {345-350}, year = {2016} }
RIS	TY - JOUR AU - Taheri D AU - Zahavi DJ AU - Del Carmen Rodriguez M AU - Meliti A AU - Rezaee N AU - Yonescu R AU - Ricardo BFP AU - Dolatkhah S AU - Ning Y AU - Bishop JA AU - Netto GJ AU - Sharma R TI - For Staining of Alk Protein, the Novel D5f3 Antibody Demonstrates Superior Overall Performance in Terms of Intensity and Extent of Staining in Comparison to the Currently Used Alk1 Antibody JO - Virchows Archiv VL - 469 IS - 3 SP - 345 EP - 350 PY - 2016 ER -

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