Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Blood Igms From Healthy Donors and Patients With Systemic Lupus Erythematosus Reduce the Inflammatory Properties of Platelets From Healthy Donors Publisher Pubmed



Alipour R1 ; Sereshki N2 ; Rafiee M3 ; Mofid MR4 ; Alsahebfosoul F1 ; Pourazar A5
Authors
Show Affiliations
Authors Affiliations
  1. 1. Immunology Department, Medical School, Isfahan University of Medical Sciences, Hezar Jerib Street, Isfahan, Iran
  2. 2. Asadabad School of Medical Sciences, Hamadan, Asadabad, Iran
  3. 3. Cellular and Molecular Research Center, Department of Immunology, Medical School, Birjand University of Medical Sciences, Ghafari Street, Southern Khorasan, Birjand, Iran
  4. 4. Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Hezar Jerib Street, Isfahan, Iran
  5. 5. Department of Immunology, Medical School, Isfahan University of Medical Sciences, Hezar Jerib Street, Isfahan, 81746-73695, Iran

Source: Immunobiology Published:2022


Abstract

Uncontrolled inflammation is the underlining mechanism of many human diseases and the increasing prevalence of such diseases mandate to develop new anti-inflammatory treatments. Utilizing the anti-inflammatory properties as well as other protective/beneficial features of natural IgMs (nIgMs) for treatment of human disorders seems as an easily accessible goal by the use of blood-purified IgMs as an alternative for polyclonal nIgMs. Despite the other blood cells, the functions of platelets have not been inspected under the influence of blood IgMs adequately. However, platelets, the second most numerous blood cells, are involved in the pathology of many inflammatory disorders through the production/expression of many inflammatory molecules. Thus, in the present study, we purified IgMs from serum of healthy donors and plasma of patients with systemic lupus erythematosus (SLE). Subsequently, we carried out comparative analysis of the inflammatory functions of normal platelets (P-selectin expression, GPIIb/IIIa activation, and secretion of soluble CD40L and TNF-α) that were stimulated by SLE microparticles (as key endogenous inflammation-drivers) in the presence or absence of the two IgM preparations; one with normal level of nIgMs (healthy blood IgMs) and the other with likely altered nIgM content (SLE blood IgMs). Both blood IgM preparations could suppress the elevated activation parameters of platelets in response to SLE microparticles. Additionally, the impact of SLE blood IgMs on the platelets was not superior to that of normal blood IgMs. The anti-inflammatory effects of blood IgMs on the activated platelets have been shown for the first time in the present study. Thus, this study provides evidence in favor of use of healthy blood IgMs as an anti-inflammatory therapy in clinical settings. © 2022
Related Docs
1. The in Vitro Effects of Vitamin 1, 25(Oh) 2 D3 on Expression of Cytokines: In New-Onset Systemic Lupus Erythematosus Patients, Lupus (2022)
2. The Impact of Neutrophil Extracellular Trap From Patients With Systemic Lupus Erythematosus on the Viability, Cd11b Expression and Oxidative Burst of Healthy Neutrophils, BMC Immunology (2021)
3. Tnf-Alpha Production by Peripheral Blood Monocytes in Multiple Sclerosis Patients and Healthy Controls, Immunological Investigations (2015)
4. Roles of Gm-Csf in the Pathogenesis of Autoimmune Diseases: An Update, Frontiers in Immunology (2019)
5. Role of Platelets in Neuroinflammation: A Wide-Angle Perspective, Journal of Neuroinflammation (2010)
Experts (# of related papers)
View all Related Experts
Masoud Etemadifar (2)
Nafiseh Esmaeil (1)