An Advanced Uracil Dna Glycosylase-Supplemented Loop-Mediated Isothermal Amplification (Udg-Lamp) Technique Used in the Sensitive and Specific Detection of Cryptosporidium Parvum, Cryptosporidium Hominis, and Cryptosporidium Meleagridis in Aids Patients

An Advanced Uracil Dna Glycosylase-Supplemented Loop-Mediated Isothermal Amplification (Udg-Lamp) Technique Used in the Sensitive and Specific Detection of Cryptosporidium Parvum, Cryptosporidium Hominis, and Cryptosporidium Meleagridis in Aids Patients Publisher Pubmed

Fallahi S^{1, 2} ; Moosavi SF² ; Karimi A² ; Chegeni AS³ ; Saki M³ ; Namdari P⁴ ; Rashno MM⁵ ; Varzi AM⁶ ; Tarrahi MJ⁷ ; Almasian M⁸

Show Affiliations

1. Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
2. Department of Medical Parasitology and Mycology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
3. Department of Communicable Disease Control and Prevention, Deputy of Health, Lorestan University of Medical Sciences, Khorramabad, Iran
4. Medical Student, Faculty of Medicine, University of Debrecen, Hungary
5. Department of Biotechnology, Islamic Azad University, Damghan Branch, Damghan, Iran
6. Department of Immunology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
7. Department of Epidemiology and Statistics, Behavioral Sciences Research Center, Isfahan University of Medical Science, Isfahan, Iran
8. School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran

Source: Diagnostic Microbiology and Infectious Disease Published:2018

Abstract

The rapid and accurate detection of Cryptosporidium spp. is critically important for the prevention and timely treatment of cryptosporidiosis in AIDS patients (APs). This study was conducted to examine a UDG-LAMP technique for the first time to diagnose cryptosporidiosis in APs. After collecting demographic and clinical data, three stool samples were collected from the participants (120 volunteering APs). The microscopic examination of stained smears using the acid-fast method and the UDG-LAMP assay were performed for each sample. 10% of APs were infected with Cryptosporidium spp. The number of detected cryptosporidiosis cases using the acid-fast staining and UDG-LAMP methods were significantly different (P < 0.001). Diarrhea and weight loss were found to be significantly associated with cryptosporidiosis in patients (P < 0.05). The pretreatment of LAMP reagents with UDG successfully eliminated the likelihood of product re-amplification remaining from previous reactions. The UDG-LAMP technique could detect cryptosporidiosis in APs with high sensitivity and rapidity without carryover contamination. © 2017 Elsevier Inc.

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Mahnaz Nikaeen (4)

Nader Pestechian (4)

Style	Citing Format
MLA	Fallahi S, et al.. "An Advanced Uracil Dna Glycosylase-Supplemented Loop-Mediated Isothermal Amplification (Udg-Lamp) Technique Used in the Sensitive and Specific Detection of Cryptosporidium Parvum, Cryptosporidium Hominis, and Cryptosporidium Meleagridis in Aids Patients." Diagnostic Microbiology and Infectious Disease, vol. 91, no. 1, 2018, pp. 6-12.
APA	Fallahi S, Moosavi SF, Karimi A, Chegeni AS, Saki M, Namdari P, Rashno MM, Varzi AM, Tarrahi MJ, Almasian M (2018). An Advanced Uracil Dna Glycosylase-Supplemented Loop-Mediated Isothermal Amplification (Udg-Lamp) Technique Used in the Sensitive and Specific Detection of Cryptosporidium Parvum, Cryptosporidium Hominis, and Cryptosporidium Meleagridis in Aids Patients. Diagnostic Microbiology and Infectious Disease, 91(1), 6-12.
Chicago	Fallahi S, Moosavi SF, Karimi A, Chegeni AS, Saki M, Namdari P, Rashno MM, Varzi AM, Tarrahi MJ, Almasian M. "An Advanced Uracil Dna Glycosylase-Supplemented Loop-Mediated Isothermal Amplification (Udg-Lamp) Technique Used in the Sensitive and Specific Detection of Cryptosporidium Parvum, Cryptosporidium Hominis, and Cryptosporidium Meleagridis in Aids Patients." Diagnostic Microbiology and Infectious Disease 91, no. 1 (2018): 6-12.
Harvard	Fallahi S et al. (2018) 'An Advanced Uracil Dna Glycosylase-Supplemented Loop-Mediated Isothermal Amplification (Udg-Lamp) Technique Used in the Sensitive and Specific Detection of Cryptosporidium Parvum, Cryptosporidium Hominis, and Cryptosporidium Meleagridis in Aids Patients', Diagnostic Microbiology and Infectious Disease, 91(1), pp. 6-12.
Vancouver	Fallahi S, Moosavi SF, Karimi A, Chegeni AS, Saki M, Namdari P, et al.. An Advanced Uracil Dna Glycosylase-Supplemented Loop-Mediated Isothermal Amplification (Udg-Lamp) Technique Used in the Sensitive and Specific Detection of Cryptosporidium Parvum, Cryptosporidium Hominis, and Cryptosporidium Meleagridis in Aids Patients. Diagnostic Microbiology and Infectious Disease. 2018;91(1):6-12.
BibTex	@article{ author = {Fallahi S and Moosavi SF and Karimi A and Chegeni AS and Saki M and Namdari P and Rashno MM and Varzi AM and Tarrahi MJ and Almasian M}, title = {An Advanced Uracil Dna Glycosylase-Supplemented Loop-Mediated Isothermal Amplification (Udg-Lamp) Technique Used in the Sensitive and Specific Detection of Cryptosporidium Parvum, Cryptosporidium Hominis, and Cryptosporidium Meleagridis in Aids Patients}, journal = {Diagnostic Microbiology and Infectious Disease}, volume = {91}, number = {1}, pages = {6-12}, year = {2018} }
RIS	TY - JOUR AU - Fallahi S AU - Moosavi SF AU - Karimi A AU - Chegeni AS AU - Saki M AU - Namdari P AU - Rashno MM AU - Varzi AM AU - Tarrahi MJ AU - Almasian M TI - An Advanced Uracil Dna Glycosylase-Supplemented Loop-Mediated Isothermal Amplification (Udg-Lamp) Technique Used in the Sensitive and Specific Detection of Cryptosporidium Parvum, Cryptosporidium Hominis, and Cryptosporidium Meleagridis in Aids Patients JO - Diagnostic Microbiology and Infectious Disease VL - 91 IS - 1 SP - 6 EP - 12 PY - 2018 ER -

Style

Citing Format

MLA

Fallahi S, et al.. "An Advanced Uracil Dna Glycosylase-Supplemented Loop-Mediated Isothermal Amplification (Udg-Lamp) Technique Used in the Sensitive and Specific Detection of Cryptosporidium Parvum, Cryptosporidium Hominis, and Cryptosporidium Meleagridis in Aids Patients." Diagnostic Microbiology and Infectious Disease, vol. 91, no. 1, 2018, pp. 6-12.

APA

Fallahi S, Moosavi SF, Karimi A, Chegeni AS, Saki M, Namdari P, Rashno MM, Varzi AM, Tarrahi MJ, Almasian M (2018). An Advanced Uracil Dna Glycosylase-Supplemented Loop-Mediated Isothermal Amplification (Udg-Lamp) Technique Used in the Sensitive and Specific Detection of Cryptosporidium Parvum, Cryptosporidium Hominis, and Cryptosporidium Meleagridis in Aids Patients. Diagnostic Microbiology and Infectious Disease, 91(1), 6-12.

Chicago

Fallahi S, Moosavi SF, Karimi A, Chegeni AS, Saki M, Namdari P, Rashno MM, Varzi AM, Tarrahi MJ, Almasian M. "An Advanced Uracil Dna Glycosylase-Supplemented Loop-Mediated Isothermal Amplification (Udg-Lamp) Technique Used in the Sensitive and Specific Detection of Cryptosporidium Parvum, Cryptosporidium Hominis, and Cryptosporidium Meleagridis in Aids Patients." Diagnostic Microbiology and Infectious Disease 91, no. 1 (2018): 6-12.

Harvard

Fallahi S et al. (2018) 'An Advanced Uracil Dna Glycosylase-Supplemented Loop-Mediated Isothermal Amplification (Udg-Lamp) Technique Used in the Sensitive and Specific Detection of Cryptosporidium Parvum, Cryptosporidium Hominis, and Cryptosporidium Meleagridis in Aids Patients', Diagnostic Microbiology and Infectious Disease, 91(1), pp. 6-12.

Vancouver

Fallahi S, Moosavi SF, Karimi A, Chegeni AS, Saki M, Namdari P, et al.. An Advanced Uracil Dna Glycosylase-Supplemented Loop-Mediated Isothermal Amplification (Udg-Lamp) Technique Used in the Sensitive and Specific Detection of Cryptosporidium Parvum, Cryptosporidium Hominis, and Cryptosporidium Meleagridis in Aids Patients. Diagnostic Microbiology and Infectious Disease. 2018;91(1):6-12.

BibTex

@article{ author = {Fallahi S and Moosavi SF and Karimi A and Chegeni AS and Saki M and Namdari P and Rashno MM and Varzi AM and Tarrahi MJ and Almasian M}, title = {An Advanced Uracil Dna Glycosylase-Supplemented Loop-Mediated Isothermal Amplification (Udg-Lamp) Technique Used in the Sensitive and Specific Detection of Cryptosporidium Parvum, Cryptosporidium Hominis, and Cryptosporidium Meleagridis in Aids Patients}, journal = {Diagnostic Microbiology and Infectious Disease}, volume = {91}, number = {1}, pages = {6-12}, year = {2018} }

RIS

TY - JOUR
AU - Fallahi S
AU - Moosavi SF
AU - Karimi A
AU - Chegeni AS
AU - Saki M
AU - Namdari P
AU - Rashno MM
AU - Varzi AM
AU - Tarrahi MJ
AU - Almasian M
TI - An Advanced Uracil Dna Glycosylase-Supplemented Loop-Mediated Isothermal Amplification (Udg-Lamp) Technique Used in the Sensitive and Specific Detection of Cryptosporidium Parvum, Cryptosporidium Hominis, and Cryptosporidium Meleagridis in Aids Patients
JO - Diagnostic Microbiology and Infectious Disease
VL - 91
IS - 1
SP - 6
EP - 12
PY - 2018
ER -

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