Vitamin D3 Induces the Expression of Membrane Progesterone Receptors (Mprs) on Naive Cd4 + T Lymphocyte Cells in Women of Reproductive Age

Vitamin D3 Induces the Expression of Membrane Progesterone Receptors (Mprs) on Naive Cd4 + T Lymphocyte Cells in Women of Reproductive Age Publisher Pubmed

Rafiee M¹ ; Naseri M² ; Akbarifakhrabadi M³ ; Motamedi N⁴ ; Ghahiri A⁵ ; Mehrabian F⁵ ; Rezaei A^{1, 6}

Show Affiliations

1. School of Medicine, Isfahan University of Medical Sciences, Department of Immunology, Isfahan, Iran
2. Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran
3. Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Iran
4. School of Medicine, Isfahan University of Medical Sciences, Department of Community Medicine, Isfahan, Iran
5. Al-Zahra University Hospital, Isfahan University of Medical Sciences, Department of Gynecology and Obstetrics, Isfahan, Iran
6. Acquired Immunodeficiency Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Source: International Immunopharmacology Published:2019

Abstract

Objective: Vitamin D3 and progesterone (P4) both belong to steroid hormones. These hormones have effects on the function of each other in different ways. The immunomodulatory activity of vitamin D3 and P4 and their role in inducing maternal tolerance for fetus have been shown in various studies. The purpose of this study was to evaluate the effect of vitamin D3 on the expression of membrane progesterone receptors (mPRs) on CD4 + T cells. Materials and methods: Naive CD4 + T cells were isolated from peripheral blood of 38 healthy women of childbearing age. After stimulating by anti-CD3 and anti-CD28 monoclonal antibodies (mAb), these cells were exposed to either various concentrations of vitamin D3 or no exposure at all in a culture medium at 37 °C for 3 days. In the final stage, the mean fluorescence intensity (MFI) of mPRα and mPRβ were evaluated using polyclonal and monoclonal antibodies and several gating strategies on CD4 + T cells. Results: Vitamin D3 significantly increased the expression of mPR α and mPR β on the surface of CD4 + T cells (p ≤ 0.05). Conclusion: The present study demonstrated the potential effect of vitamin D3 on increasing the expression of P4 receptors on CD4 + T cells. This study shows a new aspect of correlation between vitamin D3 and P4 that may influence P4 performance. Therefore, our findings suggest that the appropriate level of this vitamin may affect the optimum P4 immunomodulatory activity during pregnancy. © 2019

Related Docs

View other Related Docs

1. Altered Th17/Treg Ratio in Recurrent Miscarriage After Treatment With Paternal Lymphocytes and Vitamin D3: A Doubleblind Placebo-Controlled Study, Iranian Journal of Immunology (2015)

2. Progesterone-Induced Blocking Factor (Pibf) Influences the Expression of Membrane Progesterone Receptors (Mprs) on Peripheral Cd4+ T Lymphocyte Cells in Normal Fertile Females, Hormones (2021)

3. Impact of Vitamin C on Gene Expression Profile of Inflammatory and Anti-Inflammatory Cytokines in the Male Partners of Couples With Recurrent Pregnancy Loss, International Journal of Inflammation (2022)

4. The in Vitro Effects of Vitamin 1, 25(Oh) 2 D3 on Expression of Cytokines: In New-Onset Systemic Lupus Erythematosus Patients, Lupus (2022)

5. Comparison of Sun Exposure Versus Vitamin D Supplementation for Pregnant Women With Vitamin D Deficiency, Journal of Maternal-Fetal and Neonatal Medicine (2019)

6. Progesterone Decreases Viability and up Regulates Membrane Progesterone Receptors Expression on the Human Chronic Myeloid Leukemia Cell Line, Cancer Genetics (2024)

7. Evaluation of the Natural Killer Cell Subsets and Their Relationship With Serum Interferon Gamma and Vitamin D Levels in Women With Stages Iii and Iv Endometriosis: A Case-Control Study, International Journal of Reproductive BioMedicine (2024)

8. Vitamin D Supplementation for Premenstrual Syndrome-Related Inflammation and Antioxidant Markers in Students With Vitamin D Deficient: A Randomized Clinical Trial, Scientific Reports (2019)

Experts (# of related papers)

View all Related Experts

Reza Amani (2)

Abbas Rezaei (2)

Style	Citing Format
MLA	Rafiee M, et al.. "Vitamin D3 Induces the Expression of Membrane Progesterone Receptors (Mprs) on Naive Cd4 + T Lymphocyte Cells in Women of Reproductive Age." International Immunopharmacology, vol. 72, no. , 2019, pp. 55-61.
APA	Rafiee M, Naseri M, Akbarifakhrabadi M, Motamedi N, Ghahiri A, Mehrabian F, Rezaei A (2019). Vitamin D3 Induces the Expression of Membrane Progesterone Receptors (Mprs) on Naive Cd4 + T Lymphocyte Cells in Women of Reproductive Age. International Immunopharmacology, 72(), 55-61.
Chicago	Rafiee M, Naseri M, Akbarifakhrabadi M, Motamedi N, Ghahiri A, Mehrabian F, Rezaei A. "Vitamin D3 Induces the Expression of Membrane Progesterone Receptors (Mprs) on Naive Cd4 + T Lymphocyte Cells in Women of Reproductive Age." International Immunopharmacology 72, no. (2019): 55-61.
Harvard	Rafiee M et al. (2019) 'Vitamin D3 Induces the Expression of Membrane Progesterone Receptors (Mprs) on Naive Cd4 + T Lymphocyte Cells in Women of Reproductive Age', International Immunopharmacology, 72(), pp. 55-61.
Vancouver	Rafiee M, Naseri M, Akbarifakhrabadi M, Motamedi N, Ghahiri A, Mehrabian F, et al.. Vitamin D3 Induces the Expression of Membrane Progesterone Receptors (Mprs) on Naive Cd4 + T Lymphocyte Cells in Women of Reproductive Age. International Immunopharmacology. 2019;72():55-61.
BibTex	@article{ author = {Rafiee M and Naseri M and Akbarifakhrabadi M and Motamedi N and Ghahiri A and Mehrabian F and Rezaei A}, title = {Vitamin D3 Induces the Expression of Membrane Progesterone Receptors (Mprs) on Naive Cd4 + T Lymphocyte Cells in Women of Reproductive Age}, journal = {International Immunopharmacology}, volume = {72}, number = {}, pages = {55-61}, year = {2019} }
RIS	TY - JOUR AU - Rafiee M AU - Naseri M AU - Akbarifakhrabadi M AU - Motamedi N AU - Ghahiri A AU - Mehrabian F AU - Rezaei A TI - Vitamin D3 Induces the Expression of Membrane Progesterone Receptors (Mprs) on Naive Cd4 + T Lymphocyte Cells in Women of Reproductive Age JO - International Immunopharmacology VL - 72 IS - SP - 55 EP - 61 PY - 2019 ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract