Coordinated Modification in Expression Levels of Hspa1a/B, Dgkh, and Notch2 in Parkinson’S Patients’ Blood and Substantia Nigra As a Diagnostic Sign: The Transcriptomes’ Relationship

Coordinated Modification in Expression Levels of Hspa1a/B, Dgkh, and Notch2 in Parkinson’S Patients’ Blood and Substantia Nigra As a Diagnostic Sign: The Transcriptomes’ Relationship Publisher Pubmed

Asad Samani L¹ ; Ghaedi K² ; Majd A³ ; Peymani M⁴ ; Etemadifar M⁵

Show Affiliations

1. Department of Cellular and Molecular Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran
2. Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Azadi Sq., Hezar Jerib Ave, P.O. Code, Isfahan, 81746-73441, Iran
3. Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran
4. Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
5. Department of Neurology and Isfahan Neurosurgery Research Center, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Neurological Sciences Published:2023

Abstract

Background: Diagnosis of Parkinson’s disease (PD) is associated with a vast number of challenges. This study aimed to assess the overlap of PD patients’ transcriptomes in the substantia nigra (SN) with peripheral blood mononuclear cells (PBMCs) to discover potential biomarkers for diagnosis. Methods: GEO data were used to select genes with significant changes in expression level in the SN region and eligible studies. Also, transcriptome data related to blood of PD patients with other neurodegenerative diseases (ND) was considered. Differential expression genes between PD and control were evaluated in the SN and blood, and RT-qPCR was applied to validate the findings. Results: At the expression level, no significant similarity in long non-coding RNA was found between the patients’ SN and blood. While in silico results revealed 16 common mRNAs in SN and blood with significant expression levels. Among all overexpressed mRNAs, HSPA1A/B expression level had the highest expression difference between control and PD samples. Moreover, DGKH had the highest score of down-regulated genes in both blood and SN. The NOTCH pathway had the highest score pathway among up-regulated pathways, and the expression levels of NOTCH2, H4C8, and H2BC21 associated with this pathway had the most ability to separate the control and PD populations. Furthermore, RT-qPCR results revealed that HSPA1A/B, NOTCH2, and H4C8 were overexpressed in PD PBMCs, while DGKH expression levels were lower compared to controls. Conclusion: Our findings indicate that expression levels of HSPA1A/B, DGKH, and NOTCH2 could be applied as candidate biomarkers to diagnose PD patients in the SN region and PBMCs. © 2023, Fondazione Societa Italiana di Neurologia.

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Ahmad Chitsaz (3)

Masoud Etemadifar (3)

Style	Citing Format
MLA	Asad Samani L, et al.. "Coordinated Modification in Expression Levels of Hspa1a/B, Dgkh, and Notch2 in Parkinson’S Patients’ Blood and Substantia Nigra As a Diagnostic Sign: The Transcriptomes’ Relationship." Neurological Sciences, vol. 44, no. 8, 2023, pp. 2753-2761.
APA	Asad Samani L, Ghaedi K, Majd A, Peymani M, Etemadifar M (2023). Coordinated Modification in Expression Levels of Hspa1a/B, Dgkh, and Notch2 in Parkinson’S Patients’ Blood and Substantia Nigra As a Diagnostic Sign: The Transcriptomes’ Relationship. Neurological Sciences, 44(8), 2753-2761.
Chicago	Asad Samani L, Ghaedi K, Majd A, Peymani M, Etemadifar M. "Coordinated Modification in Expression Levels of Hspa1a/B, Dgkh, and Notch2 in Parkinson’S Patients’ Blood and Substantia Nigra As a Diagnostic Sign: The Transcriptomes’ Relationship." Neurological Sciences 44, no. 8 (2023): 2753-2761.
Harvard	Asad Samani L et al. (2023) 'Coordinated Modification in Expression Levels of Hspa1a/B, Dgkh, and Notch2 in Parkinson’S Patients’ Blood and Substantia Nigra As a Diagnostic Sign: The Transcriptomes’ Relationship', Neurological Sciences, 44(8), pp. 2753-2761.
Vancouver	Asad Samani L, Ghaedi K, Majd A, Peymani M, Etemadifar M. Coordinated Modification in Expression Levels of Hspa1a/B, Dgkh, and Notch2 in Parkinson’S Patients’ Blood and Substantia Nigra As a Diagnostic Sign: The Transcriptomes’ Relationship. Neurological Sciences. 2023;44(8):2753-2761.
BibTex	@article{ author = {Asad Samani L and Ghaedi K and Majd A and Peymani M and Etemadifar M}, title = {Coordinated Modification in Expression Levels of Hspa1a/B, Dgkh, and Notch2 in Parkinson’S Patients’ Blood and Substantia Nigra As a Diagnostic Sign: The Transcriptomes’ Relationship}, journal = {Neurological Sciences}, volume = {44}, number = {8}, pages = {2753-2761}, year = {2023} }
RIS	TY - JOUR AU - Asad Samani L AU - Ghaedi K AU - Majd A AU - Peymani M AU - Etemadifar M TI - Coordinated Modification in Expression Levels of Hspa1a/B, Dgkh, and Notch2 in Parkinson’S Patients’ Blood and Substantia Nigra As a Diagnostic Sign: The Transcriptomes’ Relationship JO - Neurological Sciences VL - 44 IS - 8 SP - 2753 EP - 2761 PY - 2023 ER -

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