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Comparing Disk Diffusion and E-Test Methods in Reviewing Methicillin- Resistance in Staphylococcus Epidermidis Isolated From Clinical Specimens



Pishva E1 ; Havaei SA1 ; Arsalani F2
Authors
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Authors Affiliations
  1. 1. Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Isfahan Medical School Published:2011

Abstract

Background: In recent years, resistance of Staphylococcus epidermidis to methicillin has significantly increase. Therefore, reviewing methicillin resistance is necessary and crucial in determining an appropriate therapeutic pattern. This study aimed to compare disk fusion and E-test methods in reviewing methicillin resistance in Staphylococcus epidermidis isolated from clinical specimens. Methods: Determination of methicillin resistance was conducted through disk fusion and E-test methods on 146 samples of Staphylococcus epidermidis. The two methods were then compared. Findings: The resistant rates of E-test and disk fusion were 70.54% and 61.64%, respectively. The sensitivity and specificity of E-test were 95.3% and 94.7%, respectively. The corresponding values for disk fusion were 86.5% and 80.9%. Conclusion: Assessing the sensitivity and specificity of E-test and disk fusion methods showed that Etest had higher sensitivity and specificity. It is also a faster, more accurate, and more reliable method to determine methicillin resistance in Staphylococcus epidermidis. © 2011, Isfahan University of Medical Sciences(IUMS). All rights reserved.
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