Premature Senescence of T Lymphocytes From Patients With Β-Thalassemia Major

Premature Senescence of T Lymphocytes From Patients With Β-Thalassemia Major Publisher Pubmed

Gharagozloo M^{1, 2} ; Bagherpour B¹ ; Tahanian M¹ ; Oreizy F¹ ; Amirghofran Z² ; Sadeghi HMM³ ; Hourfar H⁴ ; Moayedi B¹

Source: Immunology Letters Published:2009

Abstract

Several researches have demonstrated a suppressed cell mediated immunity in patients with β-thalassemia major. To know whether the premature aging of T cells is involved in abnormalities of cell mediated immunity, the biomarkers of immunosenescence including telomerase activity, apoptosis, and the expression of CD28 and CD95 were evaluated in T lymphocytes from β-thalassemia major patients. The ex vivo spontaneous apoptosis in CD4+ or CD8+ T cells from patients and healthy subjects was assessed by an in situ TdT mediated dUTP-biotin nick end labelling (TUNEL) assay after 24 h incubation in medium. Flow cytometric data revealed that lymphocytes from β-thalassemia patients were resistant to spontaneous apoptosis compared to the normal lymphocytes. Moreover, the percentages of TUNEL+CD4+ or TUNEL+CD8+ T cells from patients were significantly lower than those control cells. Quantitative determination of telomerase activity in resting and activated T cells was performed using the Telomeric Repeat Amplification Protocol (TRAP). The results showed a decreased telomerase activity of activated T cells in patients with thalassemia major compared to that in healthy controls. However, the percentages of CD8+CD28- and CD3+CD95+ T lymphocytes were significantly higher in thalassemia patients, indicating the phenotypes associated with senescent T lymphocytes. These data provide evidences for the occurrence of accelerated aging of T cells in β-thalassemia major; possibly result in abnormal T cell function leading to suppressed cell mediated immunity. Crown Copyright © 2008.

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Style	Citing Format
MLA	Gharagozloo M, et al.. "Premature Senescence of T Lymphocytes From Patients With Β-Thalassemia Major." Immunology Letters, vol. 122, no. 1, 2009, pp. 84-88.
APA	Gharagozloo M, Bagherpour B, Tahanian M, Oreizy F, Amirghofran Z, Sadeghi HMM, Hourfar H, Moayedi B (2009). Premature Senescence of T Lymphocytes From Patients With Β-Thalassemia Major. Immunology Letters, 122(1), 84-88.
Chicago	Gharagozloo M, Bagherpour B, Tahanian M, Oreizy F, Amirghofran Z, Sadeghi HMM, Hourfar H, Moayedi B. "Premature Senescence of T Lymphocytes From Patients With Β-Thalassemia Major." Immunology Letters 122, no. 1 (2009): 84-88.
Harvard	Gharagozloo M et al. (2009) 'Premature Senescence of T Lymphocytes From Patients With Β-Thalassemia Major', Immunology Letters, 122(1), pp. 84-88.
Vancouver	Gharagozloo M, Bagherpour B, Tahanian M, Oreizy F, Amirghofran Z, Sadeghi HMM, et al.. Premature Senescence of T Lymphocytes From Patients With Β-Thalassemia Major. Immunology Letters. 2009;122(1):84-88.
BibTex	@article{ author = {Gharagozloo M and Bagherpour B and Tahanian M and Oreizy F and Amirghofran Z and Sadeghi HMM and Hourfar H and Moayedi B}, title = {Premature Senescence of T Lymphocytes From Patients With Β-Thalassemia Major}, journal = {Immunology Letters}, volume = {122}, number = {1}, pages = {84-88}, year = {2009} }
RIS	TY - JOUR AU - Gharagozloo M AU - Bagherpour B AU - Tahanian M AU - Oreizy F AU - Amirghofran Z AU - Sadeghi HMM AU - Hourfar H AU - Moayedi B TI - Premature Senescence of T Lymphocytes From Patients With Β-Thalassemia Major JO - Immunology Letters VL - 122 IS - 1 SP - 84 EP - 88 PY - 2009 ER -

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