Premature Senescence of T Lymphocytes From Patients With Β-Thalassemia Major

Premature Senescence of T Lymphocytes From Patients With Β-Thalassemia Major Publisher Pubmed

Gharagozloo M^{1, 2} ; Bagherpour B¹ ; Tahanian M¹ ; Oreizy F¹ ; Amirghofran Z² ; Sadeghi HMM³ ; Hourfar H⁴ ; Moayedi B¹

Show Affiliations

1. Department of Immunology, Isfahan University of Medical Sciences, Isfahan, Iran
2. Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
3. Department of Pharmaceutical Biotechnology, Isfahan University of Medical Sciences, Isfahan, Iran
4. Division of Thalassemia and Hemophilia, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Immunology Letters Published:2009

Abstract

Several researches have demonstrated a suppressed cell mediated immunity in patients with β-thalassemia major. To know whether the premature aging of T cells is involved in abnormalities of cell mediated immunity, the biomarkers of immunosenescence including telomerase activity, apoptosis, and the expression of CD28 and CD95 were evaluated in T lymphocytes from β-thalassemia major patients. The ex vivo spontaneous apoptosis in CD4+ or CD8+ T cells from patients and healthy subjects was assessed by an in situ TdT mediated dUTP-biotin nick end labelling (TUNEL) assay after 24 h incubation in medium. Flow cytometric data revealed that lymphocytes from β-thalassemia patients were resistant to spontaneous apoptosis compared to the normal lymphocytes. Moreover, the percentages of TUNEL+CD4+ or TUNEL+CD8+ T cells from patients were significantly lower than those control cells. Quantitative determination of telomerase activity in resting and activated T cells was performed using the Telomeric Repeat Amplification Protocol (TRAP). The results showed a decreased telomerase activity of activated T cells in patients with thalassemia major compared to that in healthy controls. However, the percentages of CD8+CD28- and CD3+CD95+ T lymphocytes were significantly higher in thalassemia patients, indicating the phenotypes associated with senescent T lymphocytes. These data provide evidences for the occurrence of accelerated aging of T cells in β-thalassemia major; possibly result in abnormal T cell function leading to suppressed cell mediated immunity. Crown Copyright © 2008.

Related Docs

View other Related Docs

1. Double-Faced Cell-Mediated Immunity in Β-Thalassemia Major: Stimulated Phenotype Versus Suppressed Activity, Annals of Hematology (2009)

2. Serum Levels of Tgfβ, Il-10, Il-17, and Il-23 Cytokines in Β-Thalassemia Major Patients: The Impact of Silymarin Therapy, Immunopharmacology and Immunotoxicology (2014)

3. Immunomodulatory Effects of Silymarin in Patients With Β-Thalassemia Major, International Immunopharmacology (2013)

4. Effects of Silymarin on the Proliferation and Glutathione Levels of Peripheral Blood Mononuclear Cells From Β-Thalassemia Major Patients, International Immunopharmacology (2006)

5. Combined Therapy of Silymarin and Desferrioxamine in Patients With Β-Thalassemia Major: A Randomized Double-Blind Clinical Trial, Fundamental and Clinical Pharmacology (2009)

6. Oxidative Stress and Age-Related Changes in T Cells: Is Thalassemia a Model of Accelerated Immune System Aging?, Central European Journal of Immunology (2016)

7. The Influence of Iron Loading and Iron Chelation on the Proliferation and Telomerase Activity of Human Peripheral Blood Mononuclear Cells, Iranian Journal of Immunology (2009)

8. Evaluating the Safety and Efficacy of Silymarin in Β-Thalassemia Patients: A Review, Hemoglobin (2015)

Experts (# of related papers)

View all Related Experts

Nafiseh Esmaeil (2)

Mohammad Reza Maracy (2)

Style	Citing Format
MLA	Gharagozloo M, et al.. "Premature Senescence of T Lymphocytes From Patients With Β-Thalassemia Major." Immunology Letters, vol. 122, no. 1, 2009, pp. 84-88.
APA	Gharagozloo M, Bagherpour B, Tahanian M, Oreizy F, Amirghofran Z, Sadeghi HMM, Hourfar H, Moayedi B (2009). Premature Senescence of T Lymphocytes From Patients With Β-Thalassemia Major. Immunology Letters, 122(1), 84-88.
Chicago	Gharagozloo M, Bagherpour B, Tahanian M, Oreizy F, Amirghofran Z, Sadeghi HMM, Hourfar H, Moayedi B. "Premature Senescence of T Lymphocytes From Patients With Β-Thalassemia Major." Immunology Letters 122, no. 1 (2009): 84-88.
Harvard	Gharagozloo M et al. (2009) 'Premature Senescence of T Lymphocytes From Patients With Β-Thalassemia Major', Immunology Letters, 122(1), pp. 84-88.
Vancouver	Gharagozloo M, Bagherpour B, Tahanian M, Oreizy F, Amirghofran Z, Sadeghi HMM, et al.. Premature Senescence of T Lymphocytes From Patients With Β-Thalassemia Major. Immunology Letters. 2009;122(1):84-88.
BibTex	@article{ author = {Gharagozloo M and Bagherpour B and Tahanian M and Oreizy F and Amirghofran Z and Sadeghi HMM and Hourfar H and Moayedi B}, title = {Premature Senescence of T Lymphocytes From Patients With Β-Thalassemia Major}, journal = {Immunology Letters}, volume = {122}, number = {1}, pages = {84-88}, year = {2009} }
RIS	TY - JOUR AU - Gharagozloo M AU - Bagherpour B AU - Tahanian M AU - Oreizy F AU - Amirghofran Z AU - Sadeghi HMM AU - Hourfar H AU - Moayedi B TI - Premature Senescence of T Lymphocytes From Patients With Β-Thalassemia Major JO - Immunology Letters VL - 122 IS - 1 SP - 84 EP - 88 PY - 2009 ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract