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Analysis of Promoter Methylation, Polymorphism and Expression Profile of Cytotoxic T-Lymphocyte-Associated Antigen-4 in Patients With Gastric Cancer Publisher Pubmed



Korditamandani DM1 ; Davani SK1 ; Baranzehi T1 ; Hemati S2
Authors
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Authors Affiliations
  1. 1. Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran
  2. 2. Department of Radiation Oncology, Isfahan University of Medical Science, Isfahan, Iran

Source: Journal of Gastrointestinal and Liver Diseases Published:2014


Abstract

Background & Aim: Cytotoxic T lymphocyte-associated antigen-4 (CTLA4) is a crucial immune-checkpoint receptor regulating T-cell activation. The current study was carried out to evaluate the function of CTLA4 gene in patients with gastric cancer. Methods: The methylation of CTLA4 gene promoter was evaluated by methylation-specific polymerase chain reaction (MSP) technique using 85 paraffin-embedded gastric cancer tissue samples and normal tissue on the tumor margins as control tissue samples. Expression analysis was performed on paraffin-embedded tissue samples (25 each of cancerous and normal tissues) using Real-time PCR. Results: Statistically significant differences were observed between the tumor and margin-cell areas with respect to promoter methylation status (OR = 4.829, 95% CI: 2.46-9.48, p < 0.001) and CTLA4 expression profile (mean ± SD = 7.56 ± 17.35, p = 0.04). Conclusion: To the best of our knowledge, the current study is the first one highlighting the association between promoter hypermethylation of CTLA4 gene, decreased CTLA4 expression, and increased risk of gastric cancer.
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