Isolation of Bacteriophages Against Multidrug Resistant Acinetobacter Baumannii Publisher



Ghajavand H¹ ; Esfahani B¹ ; Havaei A¹ ; Fazeli H¹ ; Jafari R² ; Moghim S¹ Show Affiliations
Source: Research in Pharmaceutical Sciences Published:2017

Abstract

Increasing multiple drug resistant (MDR) strains of Acinetobacter baumannii has aggravated curiosity in development of alternative therapy. Bacteriophages are often considered as alternative agents for controlling A. baumannii infections. In the present study two lytic phages for MDR A. baumannii were isolated and their efficacy and host ranges were evaluated. The phages were isolated from hospital wastewater. Electron microscopy revealed that IsfAB78 might belong to Myoviridae and IsfAB39 to Podoviridae. Initial characterization of phages showed that they have narrow host range and failed to infect relative and non- relative bacteria. Both phages decreased the A. baumannii turbidity significantly, indicating that these isolated phages may be considered as candidates for phage therapy. Ghajavand Hasan 1 Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan Esfahani Bahram 2 Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan Havaei Asghar 3 Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan Fazeli Hossein 4 Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan Jafari Reyhaneh 5 Department of Microbiology, School of Biology, Falavarjan Branch, Islamic Azad University, Isfahan Moghim Sharareh 6 Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan Perez F, Hujer AM, Hujer KM, Decker BK, Rather PN,Bonomo RA. Global challenge of multidrug - resistunat Acinetobacter baumannii. Antimicrob Agent Chemother. 2007,51(10):3471-3348. Barbolla RE, Centron D, Maimones, Rospide F, Salgueila C, Altclas J, et al. 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