The Frequency of Exon 2 Mutations of Klf6 Tumor Suppressor Gene in Prostate Cancer Specimens



Dastjerdi MN¹ ; Ramezani J² Show Affiliations
Source: Journal of Isfahan Medical School Published:2012

Abstract

Background: Prostate cancer is an important disease whose many genetic aspects are still uncovered in spite of the wide spectrum of the studies previously performed on its genetic and environmental risk factors. According to the previous studies, KLF6, a suppressor gene dealing with prostate cancer, is exposed to genetic changes. Among all these changes, mutation of exon No. 2, which is in relation with disease prognosis, is common. The few studies conducted on the mutation of KLF6 gene have shown a relation between mutation and environmental factors as well as race and geographic conditions. The goal of this study was to investigate the mutation of exon No. 2 of KLF6 gene in people who suffer from prostate cancer in city of Isfahan, Iran. Methods: The study was carried out on microscopically and pathologically proved cases of prostate cancer in Isfahan, Iran, during 2009-2011. The samples were taken by prostatectomy or biopsy from 40 patients suffering the disease. A procedure including DNA extraction, determination of quality and quantity of DNA, PCR (using specific primers to replicate exon No. 2), polyacrylamide gel electro-phoreses, and data analysis was performed on the samples to determine the mutation in exon No. 2 of KLF6 gene. Findings: Overall, 80% of the patients (32 people) were above 60 years of age. Only 17.5% of the patients (7 people) had mutation in exon No. 2 of KLF6 gene. There was no significant relation between the mutation of exon No. 2 of KLF6 gene and the age of the patients. A significant relation was found between the presence of mutation and tumor grading in the studied patients. Conclusion: The frequency of mutation in exon No. 2 of KLF6 gene was higher in cancer samples with higher grade. This finding can be a sign of weak prognosis in samples whose exon No. 2 of KLF6 gene has been mutated.