Characterization of a Bioactive Compounds Produced by Streptomyces Phaeochromogenes Nrrl B-2123



Bamzadeh Z¹ ; Baserisalehi M² ; Bahador N¹ ; Hejazi SH³ Show Affiliations
Source: Nature Environment and Pollution Technology Published:2014

Abstract

Streptomyces is the largest genus of Actinobacteria and includes aerobic, Gram-positive filamentous bacteria with high G+C content. Although, few species of Streptomyces are pathogens, they could be characterized by production of the beneficial metabolites viz., antibiotics, antifungal, antiparasitic, and immunosuppressant compounds. The present study was conducted to isolate Streptomyces from soil and characterize their bioactive compounds. In total 85 soil samples were collected and assessed for production of the bioactive compounds by Agar Well Diffusion method against antagonistic microorganisms such as Escherichia coli, Pseudomonas aeruginosa, Bacillus cereus, Staphylococcus aureus, Aspergillus niger and Candida albicans. Of all the Streptomyces isolates, one strain exhibited potent activity against Staphylococcus aureus, Bacillus cereus and Aspergillus niger. The promising strain identified using 16SrRNA gene sequencing and recognized as Streptomyces phaeochromogenes strain NRRL. Then, the growth phase of production and Arbitrary Unit (AU) of the bioactive compounds were determined, and the cured bioactive compound was purified and subjected to 1H NMR, 13C NMR and FTIR analysis for determination of the structural formula. The results obtained illustrated that high level of the bioactive compounds produced at stationary phase with Arbitrary Unit 32 AU. On the other hand, the data obtained from 1H NMR, 13C NMR and FTIR analysis exhibited a straight chain with a possible structural formula C 13H15NO3 for the bioactive compound.