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Simultaneous Analysis of Benzoic and Sorbic Acids in Orange Juice Using Thymol/ Water Natural Deep Eutectic Solvent and Dllme Method Followed by High Performance Liquid Chromatography Publisher



Mohamadi S1 ; Esfandiari Z2 ; Khodadadi M3 ; Dehaghani MST2
Authors
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Authors Affiliations
  1. 1. Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Shahre-Kord University, Shahre-Kord, Iran
  2. 2. Nutrition and Food Security Research Center, Department of Food Science and Technology, School of Nutrition and Food Science, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Core Research Facilities (CRF), Isfahan University of Medical Sciences, Isfahan, 81746 73461, Iran

Source: Journal of Food Measurement and Characterization Published:2024


Abstract

In this study, the analytical and developed method was successfully validated for the simultaneous determination of benzoic acid (BA) and Sorbic acid (SA) by natural deep eutectic solvent (DES) composed of thymol/water and dispersive liquid–liquid microextraction (DLLME) followed by HPLC/UV in orange juice samples. A response surface methodology (RSM) based on a central composite design (CCD) was applied for optimization of the main variables. The significant parameters including DES volume (600 µL), dispersive solvent volume (THF) (709 µL), sample volume (11.1 mL), salt amount (5% w/v), and vortex time (10 min) were optimized. Under the optimal conditions, the limits of detection (LOD) and limits of quantifications (LOQ) were obtained 0.468 and 1.419 mg L−1 for BA and 2.517 and 7.628 mg L−1 for SA, respectively. The recovery values were between 94 and 97%. R2 of BA and SA were 0.9940 and 0.9921, respectively. Graphical Abstract: (Figure presented.). © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024.
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