Transcriptional Noise in Intact and Tgf-Beta Treated Human Kidney Cells; the Importance of Time-Series Designs

Transcriptional Noise in Intact and Tgf-Beta Treated Human Kidney Cells; the Importance of Time-Series Designs Publisher Pubmed

Rabieian R¹ ; Moein S¹ ; Khanahmad H¹ ; Mortazavi M² ; Gheisari Y^{1, 3}

Show Affiliations

1. Department of Genetics and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran
2. Isfahan Kidney Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
3. Regenerative Medicine Lab, Isfahan Kidney Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Cell Biology International Published:2018

Abstract

The transforming growth factor (TGF)-β signaling pathway plays a key role in various cellular processes. However, insufficient knowledge about the complex and sometimes paradoxical functions of this pathway hinders its therapeutic targeting. In this study, the transcriptional profile of seven mediators and downstream elements of the TGF-β pathway were assessed in TGF-β treated and untreated human kidney derived cells for 2 weeks in a time course manner. As expected the up-regulation of ACTA2 and COL1A2 was evident in the treated cells. However, we observed remarkable fluctuations in gene expression, even in the supposedly steady states. The magnitude of noise was diverse in the examined genes. Our findings underscore the significance of time-course designs for gene expression analyses and clearly show that misleading data can be obtained in single point measurements. Furthermore, we propose specific considerations in the interpretation of time-course data in the context of noisy gene expression. © 2018 International Federation for Cell Biology

2. In Vitro Versus in Vivo Models of Kidney Fibrosis: Time-Course Experimental Design Is Crucial to Avoid Misinterpretations of Gene Expression Data, Journal of Research in Medical Sciences (2020)

3. Identification of Key Genes and Biological Regulatory Mechanisms in Diabetic Nephropathy: Meta-Analysis of Gene Expression Datasets; [Identificacion De Genes Clave Y Mecanismos Reguladores Biologicos En La Nefropatia Diabetica: Metaanalisis De Conjuntos De Datos De Expresion Genica], Nefrologia (2023)

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Yousof Gheisari (3)

Shiva Moein (1)

Style	Citing Format
MLA	Rabieian R, et al.. "Transcriptional Noise in Intact and Tgf-Beta Treated Human Kidney Cells; the Importance of Time-Series Designs." Cell Biology International, vol. 42, no. 9, 2018, pp. 1265-1269.
APA	Rabieian R, Moein S, Khanahmad H, Mortazavi M, Gheisari Y (2018). Transcriptional Noise in Intact and Tgf-Beta Treated Human Kidney Cells; the Importance of Time-Series Designs. Cell Biology International, 42(9), 1265-1269.
Chicago	Rabieian R, Moein S, Khanahmad H, Mortazavi M, Gheisari Y. "Transcriptional Noise in Intact and Tgf-Beta Treated Human Kidney Cells; the Importance of Time-Series Designs." Cell Biology International 42, no. 9 (2018): 1265-1269.
Harvard	Rabieian R et al. (2018) 'Transcriptional Noise in Intact and Tgf-Beta Treated Human Kidney Cells; the Importance of Time-Series Designs', Cell Biology International, 42(9), pp. 1265-1269.
Vancouver	Rabieian R, Moein S, Khanahmad H, Mortazavi M, Gheisari Y. Transcriptional Noise in Intact and Tgf-Beta Treated Human Kidney Cells; the Importance of Time-Series Designs. Cell Biology International. 2018;42(9):1265-1269.
BibTex	@article{ author = {Rabieian R and Moein S and Khanahmad H and Mortazavi M and Gheisari Y}, title = {Transcriptional Noise in Intact and Tgf-Beta Treated Human Kidney Cells; the Importance of Time-Series Designs}, journal = {Cell Biology International}, volume = {42}, number = {9}, pages = {1265-1269}, year = {2018} }
RIS	TY - JOUR AU - Rabieian R AU - Moein S AU - Khanahmad H AU - Mortazavi M AU - Gheisari Y TI - Transcriptional Noise in Intact and Tgf-Beta Treated Human Kidney Cells; the Importance of Time-Series Designs JO - Cell Biology International VL - 42 IS - 9 SP - 1265 EP - 1269 PY - 2018 ER -

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