The Effect of Aerobic Training on Tumor Growth and Expression of Bcl-2 Gene and Protein in Female Mice With Breast Cancer

The Effect of Aerobic Training on Tumor Growth and Expression of Bcl-2 Gene and Protein in Female Mice With Breast Cancer Publisher

Shahvali Koohshoori Y¹ ; Marandi M² ; Kargarfard M² ; Vaseghi G³ ; Moshtaghian J⁴

Show Affiliations

1. Faculty of Sport Sciences, University of Isfahan, Isfahan, Iran
2. Department of Exercise Physiology, Faculty of Sport Sciences, University of Isfahan, Isfahan, Iran
3. Department of Applied physiology, Faculty of Medical, Isfahan University of Medical Sciences, Isfahan, Iran
4. Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran

Source: Iranian Journal of Breast Diseases Published:2021

Abstract

Introduction: The aim of this study was to investigate the effect of aerobic exercise with detraining in different phases of prevention on Bcl-2 gene expression and protein. Methods: For this purpose, 32 female BALB-c mice (18-20 g) were purchased and randomly assigned to four groups of primordial prevention (A), primary prevention (B), secondary prevention (C), and control (D). Group A performed aerobic exercise for 4 weeks, followed by injection of 4T1 cells and 8 weeks of detraining after the injection. Group B performed aerobic exercise for 4 weeks immediately after the injection of 4T1 cells and then detrained for 4 weeks. Group C received a 4T1 cell injection and maintained a sedentary life for 4 weeks, followed by 4 weeks of aerobic exercise. The subjects were killed 48 hours after the last training session and detraining courses and tumor tissues were removed. Real-time polymerase chain reaction was used to measure gene expression and western blotting was used to measure protein content.The one-way ANOVA test was used to analyze the data. Results: The mean gene expression due to aerobic exercise was significantly (P<0.001) lower in groups A (0.481), B (0.323), and C (0.035) compared with group D (1.711). Also, aerobic exercise caused a significant decrease in Bcl-2 (P=0.005) protein expression in groups A (0.692), B (0.821), and C (0.670) compared with group D (1.000). It should be noted that tumor growth in experimental groups was not significantly different from the control group (P=0.092). Conclusion: Exercise may be able to reduce anti-apoptotic agents in tumor cells, leading to apoptosis of tumor cells and reduced tumor growth. © 2021 Iranian Academic Center for Education, Culture and Research. All right reserved.

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Golnaz Vaseghi (4)

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Style	Citing Format
MLA	Shahvali Koohshoori Y, et al.. "The Effect of Aerobic Training on Tumor Growth and Expression of Bcl-2 Gene and Protein in Female Mice With Breast Cancer." Iranian Journal of Breast Diseases, vol. 13, no. 4, 2021, pp. 67-76.
APA	Shahvali Koohshoori Y, Marandi M, Kargarfard M, Vaseghi G, Moshtaghian J (2021). The Effect of Aerobic Training on Tumor Growth and Expression of Bcl-2 Gene and Protein in Female Mice With Breast Cancer. Iranian Journal of Breast Diseases, 13(4), 67-76.
Chicago	Shahvali Koohshoori Y, Marandi M, Kargarfard M, Vaseghi G, Moshtaghian J. "The Effect of Aerobic Training on Tumor Growth and Expression of Bcl-2 Gene and Protein in Female Mice With Breast Cancer." Iranian Journal of Breast Diseases 13, no. 4 (2021): 67-76.
Harvard	Shahvali Koohshoori Y et al. (2021) 'The Effect of Aerobic Training on Tumor Growth and Expression of Bcl-2 Gene and Protein in Female Mice With Breast Cancer', Iranian Journal of Breast Diseases, 13(4), pp. 67-76.
Vancouver	Shahvali Koohshoori Y, Marandi M, Kargarfard M, Vaseghi G, Moshtaghian J. The Effect of Aerobic Training on Tumor Growth and Expression of Bcl-2 Gene and Protein in Female Mice With Breast Cancer. Iranian Journal of Breast Diseases. 2021;13(4):67-76.
BibTex	@article{ author = {Shahvali Koohshoori Y and Marandi M and Kargarfard M and Vaseghi G and Moshtaghian J}, title = {The Effect of Aerobic Training on Tumor Growth and Expression of Bcl-2 Gene and Protein in Female Mice With Breast Cancer}, journal = {Iranian Journal of Breast Diseases}, volume = {13}, number = {4}, pages = {67-76}, year = {2021} }
RIS	TY - JOUR AU - Shahvali Koohshoori Y AU - Marandi M AU - Kargarfard M AU - Vaseghi G AU - Moshtaghian J TI - The Effect of Aerobic Training on Tumor Growth and Expression of Bcl-2 Gene and Protein in Female Mice With Breast Cancer JO - Iranian Journal of Breast Diseases VL - 13 IS - 4 SP - 67 EP - 76 PY - 2021 ER -

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