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On the Colorimetric Method for Cholesterol Determination in the Laboratory Media Publisher



Mirlohi M1, 2 ; Madany G1 ; Hassanzadeh A1, 3 ; Yahay MJ1
Authors
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Authors Affiliations
  1. 1. Tood Security Research Center, Isfahan University of Medical Science, Iran
  2. 2. Department of Food Science, School of Nutrition and Food Science, Isfahan University of Medical Science, Iran
  3. 3. School of Health, Isfahan University of Medical Science, Iran

Source: International Journal of Biological Chemistry Published:2012


Abstract

This study aimed at the evaluation of sensitivity and reproducibility of the modified o-phthalaldehyde colorimetric method in determination of cholesterol in the Lactobacillus specific laboratory media; (de-Man Rogosa Sharpe medium). The results indicated that at the range of 0-100 mg L -1 cholesterol concentration, the quantity of measured cholesterol was affected by the type of cholesterol. Application of the method for water soluble cholesterol yielded less quantity of cholesterol in comparison to the same situation for free alcoholic cholesterol. Also, the method did not present substantial reproducibility. Therefore, cholesterol containing MRS solutions cannot be accurately distinguished using this method. © 2012 Academic Journals Inc.
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