Study of the Effects of Actinomycin and Puromycin on Estradiol-Induced Ceruloplasmin Elevation in the Male Rat



Mostaghie AA¹ ; Satari G¹ Show Affiliations
Source: Romanian Journal of Endocrinology Published:1996

Abstract

The effects of some steroid hormones on serum ceruloplasmin (ferroxidase) activity in male rats were investigated over different periods of time. The ceruloplasmin activity was elevated by 50, 72 and 62 percent, respectively, following administration of 0.5, 1.0 and 1.5 mg/kg estradiol-17β daily during 15 days. Following daily administration, of some amounts of estradiol-17β for 30 days, the enzyme activity increased by 87, 120 and 108 percent, respectively. When the rats were daily injected with 0.5, 1.0 and 1.5 mg/kg estradiol for 60 days, a 73, 102 and 75 percent increase in the activity of ceruloplasmin was noted. No significant changes were found when the rats were treated with either progesterone or testosterone under the same conditions. Castration of the rats did not have any effect on the level of serum ceruloplasmin. The response of ceruloplasmin (ferroxidase) activity to estradiol was blocked by actinomycin-D and/or puromycin. The relationship between the present study and the general endocrine control of ceruloplasmin is discussed.