Tgf B 1 Genetic Variants Are Associated With an Increased Risk of Acute Brucellosis Publisher Pubmed



Sepanjnia A¹ ; Eskandarinasab E^{2, 3} ; Moghadampour M⁴ ; Tahmasebi A⁵ ; Dahmardeh F⁶ Show Affiliations
Source: Infectious Diseases Published:2015

Abstract

Objective: Cytokines play a critical role in the regulation of the immune response against brucellosis infection, and mediate production of many pro-and anti-inflammatory signals. Transforming growth factor-beta 1 (TGFβ?1), a powerful suppressive cytokine, inhibits macrophage activation and modulates T-cell function, and plays crucial roles in regulation of microbial replication and host responses to brucella. Methods: The association of three polymorphisms in the TGFβ?1 gene ( - 509 C/T [rs1800469],+868 C/T [rs1800470], and+913 G/C [rs1800471]) in promoter, codons 10 and 25, respectively, with brucellosis infection was evaluated. This case-control study was performed on a total of 281 Iranian subjects including 153 patients with active brucellosis and 128 age-and sex-matched healthy individuals as controls. Genotyping for the TGFβ?1 - 509 C/T and+868 C/T variants was performed using tetra amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR). Also, the+913 G/C polymorphism was genotyped using an allelespecific PCR. Results: The results demonstrated that the TGFβ?1+868 C/T mutant homozygote genotype (TT vs CC), was a risk factor for developing brucellosis in the co-dominant and recessive models (odds ratio (OR)=2.60, p=0.023; OR=2.602, p=0.014, respectively). Additionally, the diplotype analyses revealed that TGFβ?1 codon 10 and 25 diplotype, TT/GG, was associated with an increased risk of brucellosis (OR=2.49, p=0.038). Other TGFβ?1 variants did not increase the risk of brucellosis infection. Conclusions: Our findings propose that TGFβ?1+868 TT genotype and TT/GG diplotype may confer increased risk of brucellosis in the examined population. © 2015 Informa Healthcare.