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Development and Validation of a Novel Rp-Hplc Method for Pharmacokinetic Studies of Gliclazide in Rat



Talar R1 ; Arshosaz J2 ; Mostafavi A2 ; Nokhodchi A3, 4
Authors
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Authors Affiliations
  1. 1. Food and Drug Laboratory, Tehran, Iran
  2. 2. Pharmaceutical Research Center, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Medway School of Pharmacy, University of Kent, Chatham ME4 4TB, Kent, Central Ave, United Kingdom
  4. 4. Drug Applied Research Centre, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

Source: Farmacia Published:2011

Abstract

A sensitive, simple and accurate reverse phase HPLC method was developed to determine plasma levels of gliclazide (GL) in rat. The method uses liquid-liquid extraction that is not expensive and ibuprofen as internal standard, that is easily available. Mean recovery for GL and internal standard was 80% and 82% respectively. Relative standard errors and CV% ranged from 0.47 to 8.99 and from 0.72 to 12.54% for intraday and interday HPLC injections, respectively. The limit of quantitation (LOQ) and the limit of detection (LOD) for GL in serum were 0.12 μg/mL and 0.06 μg/mL respectively. The developed HPLC method is a suitable and rapid method to be adopted for pharmacokinetic studies in rat and human.
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