Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
A Survey on Apoptosis and Hydatid Cyst Infertility Publisher



Rahmanidehaghani M1 ; Ghayournajafabadi Z1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Iran

Source: Journal of Isfahan Medical School Published:2021


Abstract

Background: Hydatidosis is an infection with global distribution caused by the genus of Echinococcus. Although surgery is regarded as the first choice of hydatid cyst treatment, it has not always been successful. Apoptosis has recently been studied as an important part of host innate immunity in suppressing parasites and probably, it can be an ideal mechanism for killing protoscolices of parasites. This study was designed to compare the outcomes of apoptosis in the germinal layer in infertile and fertile hydatid cysts. Methods: 6 samples of fertile and infertile cysts were used from bovine liver infected with hydatidosis. To survey gene expression of Fas-L, the mRNA was extracted from laminated-germinal layer and normal tissue of each cyst as the control, and cDNA synthesis was done. Then, the mean of gene expression in each sample along with the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene as the reference gene was measured by real-time polymerase chain reaction (RT-PCR). Moreover, DNA fragmentation was evaluated by electrophoresis. Findings: The mean expression of Fas-L gene in the laminated-germinal layer of infertile cysts was higher than fertile cysts and host normal tissue. DNA Fragmentation was seen in infertile cysts. Conclusion: Increasing the expression of Fas-L gene and DNA Fragmentation in laminated-germinal layer of infertile cysts represents that apoptosis may be involved in hydatid cyst infertility. © 2021 Isfahan University of Medical Sciences(IUMS). All rights reserved.
Related Docs
View other Related Docs
1. Apoptosis As a Potential Target to Arrest and Survival of Hydatid Cyst, Advanced Biomedical Research (2023)
2. Apoptosis Genes Expression in Bovine Lymphocytes in Response to Fertile and Infertile Hydatid Cyst Fluid, Journal of Veterinary Research (2019)
3. Evaluating the Gene Expression Level of Il-12 in the Fibrous Layer of Hepatic Hydatid Cysts Isolated From Slaughtered Animals, Comparative Clinical Pathology (2015)
4. Histopathological Study on Fibrous Layer of Hydatid Cyst in Camel, Cattle and Sheep, Journal of Isfahan Medical School (2016)
5. Antibody Response to Glycan Antigens of Hydatid Cyst Fluid, Laminated Layer and Protoscolex of Echinococcus Granulosus, Medical Journal of the Islamic Republic of Iran (2017)
6. Effect of Hydatid Cyst Fluid Antigens on Induction of Apoptosis on Breast Cancer Cells, Advanced Biomedical Research (2019)
7. Overview of Hydatid Disease in Iranian Children, Archives of Pediatric Infectious Diseases (2015)
8. Effect of Hydatid Cyst Antigens Polyspecific Antisera on Breast Cancer Cells (4T1) Growth in Cell Culture Medium, International Journal of Environmental Health Engineering (2022)
Experts (# of related papers)
View all Related Experts
Hossein Yousofi Darani (26)
Zahra Ghayour Najafabadi (6)
Other Related Docs
9. Genetic Diversity of Echinococcus Granulosus in Center of Iran, Korean Journal of Parasitology (2014)
10. First Report of Tasmanian Sheep Strain (G2) Genotype Isolated From Iranian Goat Using the High Resolution Melting (Hrm) Analysis, Gastroenterology and Hepatology from Bed to Bench (2016)
11. Il-4 Gene Expression in Adventitial Layer (Fibrous Layer) of Hepatic Ovine and Bovine Hydatid Cysts, Journal of Parasitic Diseases (2016)
12. Diagnostic Value of Hydatid Cyst Antigens Using Western Blotting Method, Jundishapur Journal of Microbiology (2010)
13. Immunological Diagnosis of Human Hydatid Cyst Using Western Immunoblotting Technique, Journal of Research in Medical Sciences (2016)
14. Molecular Characterization of Human and Animal Echinococcus Granulosus Isolates in Isfahan, Iran, Acta Tropica (2011)
15. Expression of Matrix Metalloproteinases in Human Cystic Echinococcosis, Current Molecular Medicine (2024)
16. Immunohistochemical Observation of Local Inflammatory Cell Infiltration in the Host-Tissue Reaction Site of Human Hydatid Cysts, Journal of Helminthology (2019)
17. Molecular Epidemiology of Echinococcus Granulosus Strains in Domestic Herbivores of Lorestan, Iran, Jundishapur Journal of Microbiology (2011)
18. Effect of Two Hydatid Cyst Antigens on the Growth of Melanoma Cancer in C57/Black Mice, Journal of Parasitic Diseases (2016)
19. Albendazole and Treatment of Hydatid Cyst: Review of the Literature, Infectious Disorders - Drug Targets (2019)
20. Sensitive Detection of Specific Cell-Free Dna in Serum Samples From Sheep With Cystic Echinococcosis, PLoS Neglected Tropical Diseases (2023)
21. Investigating the Reaction of Antisera Against Hydatid Cyst Antigens With the Surface of Breast Cancer (4T1), Melanoma (B16f10), and Normal Spleen Cells Using Flow Cytometry Technique, Journal of Isfahan Medical School (2018)
22. Identifying the Most Effective Hydatid Cyst Fluid Fraction for Anticancer Vaccination of 4T1 Breast Tumor-Bearing Mice, International Journal of Preventive Medicine (2019)
23. Epidemiological, Clinical, and Paraclinical Study of Hydatidosis in Isfahan City, Iran, During the Years 2008-2016, Journal of Isfahan Medical School (2021)
24. Genotype Identification of Human Cystic Echinococcosis in Isfahan, Central Iran, Parasitology Research (2010)
25. Isolated Human and Livestock Echinococcus Granulosus Genotypes Using Real-Time Pcr of Cox1 Gene in Northeast Iran, Acta Parasitologica (2019)
26. Monoclonal Antibodies Production Against a 40Kda Band of Hydatid Cyst Fluid, Recent Patents on Biotechnology (2018)
27. Effect of Alive Protoscoleces of Hydatid Cyst on the Growth of Melanoma Cells in Mouse Model, Journal of Isfahan Medical School (2014)
28. Echinococcus Multilocularis and Echinococcus Granulosus in Canines in North-Khorasan Province, Northeastern Iran, Identified Using Morphology and Genetic Characterization of Mitochondrial Dna, Parasites and Vectors (2019)
29. A Slaughterhouse Study on Prevalence of Some Helminths of Cattle in Lorestan Provience, West Iran, Asian Pacific Journal of Tropical Disease (2014)
30. Genetic Characterization of Echinococcus Granulosus Strains Isolated From Humans Based on Nad1 and Cox1 Gene Analysis in Isfahan, Central Iran, Journal of Helminthology (2018)
31. Echinococcus Granulosus Genotypes in Iran, Gastroenterology and Hepatology from Bed to Bench (2014)
32. Echhinococcus Granulosus Lnfection in Mice Following Lmmunization With Protoscoleces Antigens, Journal of Isfahan Medical School (2009)
33. Echinococcus Granulosus Sheep Strain (G1) As the Predominant Genotype in Definitive Host (Dogs) Isolates in Northeastern Iran, Veterinary Parasitology: Regional Studies and Reports (2024)
34. Stability Determination of Recombinant Echinococcus Granulosus Antigen B Kit by Physical and Bacteriostatical Methods, Journal of Isfahan Medical School (2013)
35. New Mitogenome and Nuclear Evidence on the Phylogeny and Taxonomy of the Highly Zoonotic Tapeworm Echinococcus Granulosus Sensu Stricto, Infection, Genetics and Evolution (2017)
36. Diagnostic Value of Echinococcus Granulosus Recombinant Protein B-Containing Kit in Comparison With the Euroimmun Kit, Journal of Isfahan Medical School (2013)
37. A New Primer Pair in Its1 Region for Molecular Studies on Echinococcus Granulosus, Iranian Journal of Public Health (2007)
38. Evaluation of the Slide Latex Agglutination and Intradermal (Casoni) Tests for Echinococcosis, American Journal of Tropical Medicine and Hygiene (1976)
39. Inhibition of Mouse Colon Cancer Growth Following Immunotherapy With a Fraction of Hydatid Cyst Fluid, Experimental Parasitology (2023)
40. Multi-Epitope Vaccine Against Cystic Echinococcosis Using Immunodominant Epitopes From Ega31 and Egg1y162 Antigens, Informatics in Medicine Unlocked (2020)
41. Rapid Identification of Echinococcus Granulosus and E. Canadensis Using High-Resolution Melting (Hrm) Analysis by Focusing on a Single Nucleotide Polymorphism, Japanese Journal of Infectious Diseases (2016)
42. Analysis of the Cox1 Gene in Echinococcus Granulosus From Sheep in Northeast Iran Using Pcr High-Resolution Melting (Qpcr-Hrm) Curve Analysis, Tropical Biomedicine (2018)
43. Scolicidal Agents for Protoscolices of Echinococcus Granulosus Hydatid Cyst: Review of Literature, Journal of Research in Medical Sciences (2017)
44. Bio-Membrane Selex As a New Approach for Selecting Ss-Dna Aptamers That Bind to the Hydatid Cyst Laminated Layer, Current Molecular Medicine (2023)
45. Effect of Hydatid Cyst Antigens on Inhibition of Melanoma Cancer Growth in Mouse Model, Cellular and Molecular Biology (2018)
46. Molecular Studies on Cystic Echinococcosis of Camel (Camelus Dromedarius) and Report of Echinococcus Ortleppi in Iran, Iranian Journal of Parasitology (2017)