Construction of a Sensitive and Specific Lead Biosensor Using a Genetically Engineered Bacterial System With a Luciferase Gene Reporter Controlled by Pbr and Cada Promoters

Construction of a Sensitive and Specific Lead Biosensor Using a Genetically Engineered Bacterial System With a Luciferase Gene Reporter Controlled by Pbr and Cada Promoters Publisher Pubmed

Nourmohammadi E^{1, 2} ; Hosseinkhani S³ ; Nedaeinia R⁴ ; Khoshdelsarkarizi H² ; Nedaeinia M⁵ ; Ranjbar M⁶ ; Ebrahimi N⁷ ; Farjami Z¹ ; Nourmohammadi M⁸ ; Mahmoudi A¹ ; Goli M⁹ ; Ferns GA¹⁰ ; Sadeghizadeh M¹¹

Show Affiliations

1. Department of Medical Biotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2. Research Center of Advanced Technologies in Medicine, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
3. Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
4. Pediatric Inherited Diseases Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran
5. Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
6. Advanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran
7. Laboratory of Cedars-Sinai Medical Center, Los Angeles, CA, United States
8. Department of Health Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
9. Department of Food Science and Technology, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
10. Brighton and Sussex Medical School, Division of Medical Education, Falmer, Brighton, Sussex, BN1 9PH, United Kingdom
11. Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

Source: BioMedical Engineering Online Published:2020

Abstract

Background: A bacterial biosensor refers to genetically engineered bacteria that produce an assessable signal in the presence of a physical or chemical agent in the environment. Methods: We have designed and evaluated a bacterial biosensor expressing a luciferase reporter gene controlled by pbr and cadA promoters in Cupriavidus metallidurans (previously termed Ralstonia metallidurans) containing the CH34 and pI258 plasmids of Staphylococcus aureus, respectively, and that can be used for the detection of heavy metals. In the present study, we have produced and evaluated biosensor plasmids designated pGL3-luc/pbr biosensor and pGL3-luc/cad biosensor, that were based on the expression of luc+ and under the control of the cad promoter and the cadC gene of S. aureus plasmid pI258 and pbr promoter and pbrR gene from plasmid pMOL30 of Cupriavidus metallidurans. Results: We found that the pGL3-luc/pbr biosensor may be used to measure lead concentrations between 1–100 μM in the presence of other metals, including zinc, cadmium, tin and nickel. The latter metals did not result in any significant signal. The pGL3-luc/cad biosensor could detect lead concentrations between 10 nM to 10 μM. Conclusions: This biosensor was found to be specific for measuring lead ions in both environmental and biological samples. © 2020, The Author(s).

2. Resazurin Reduction Assay, a Useful Tool for Assessment of Heavy Metal Toxicity in Acidic Conditions, Environmental Monitoring and Assessment (2015)

3. Aptamer-Functionalized Localized Surface Plasmon Resonance Sensor for the Rapid and Affordable Detection of Staphylococcus Aureus Alpha-Toxin, Optik (2022)

4. Bacteria in Heavy Metal Remediation and Nanoparticle Biosynthesis, ACS Sustainable Chemistry and Engineering (2020)

Experts (# of related papers)

View all Related Experts

Mahnaz Nikaeen (2)

Mohammad Mehdi Amin (2)

Style	Citing Format
MLA	Nourmohammadi E, et al.. "Construction of a Sensitive and Specific Lead Biosensor Using a Genetically Engineered Bacterial System With a Luciferase Gene Reporter Controlled by Pbr and Cada Promoters." BioMedical Engineering Online, vol. 19, no. 1, 2020, pp. -.
APA	Nourmohammadi E, Hosseinkhani S, Nedaeinia R, Khoshdelsarkarizi H, Nedaeinia M, Ranjbar M, Ebrahimi N, Farjami Z, Nourmohammadi M, Mahmoudi A, Goli M, Ferns GA, Sadeghizadeh M (2020). Construction of a Sensitive and Specific Lead Biosensor Using a Genetically Engineered Bacterial System With a Luciferase Gene Reporter Controlled by Pbr and Cada Promoters. BioMedical Engineering Online, 19(1), -.
Chicago	Nourmohammadi E, Hosseinkhani S, Nedaeinia R, Khoshdelsarkarizi H, Nedaeinia M, Ranjbar M, Ebrahimi N, et al.. "Construction of a Sensitive and Specific Lead Biosensor Using a Genetically Engineered Bacterial System With a Luciferase Gene Reporter Controlled by Pbr and Cada Promoters." BioMedical Engineering Online 19, no. 1 (2020): -.
Harvard	Nourmohammadi E et al. (2020) 'Construction of a Sensitive and Specific Lead Biosensor Using a Genetically Engineered Bacterial System With a Luciferase Gene Reporter Controlled by Pbr and Cada Promoters', BioMedical Engineering Online, 19(1), pp. -.
Vancouver	Nourmohammadi E, Hosseinkhani S, Nedaeinia R, Khoshdelsarkarizi H, Nedaeinia M, Ranjbar M, et al.. Construction of a Sensitive and Specific Lead Biosensor Using a Genetically Engineered Bacterial System With a Luciferase Gene Reporter Controlled by Pbr and Cada Promoters. BioMedical Engineering Online. 2020;19(1):-.
BibTex	@article{ author = {Nourmohammadi E and Hosseinkhani S and Nedaeinia R and Khoshdelsarkarizi H and Nedaeinia M and Ranjbar M and Ebrahimi N and Farjami Z and Nourmohammadi M and Mahmoudi A and Goli M and Ferns GA and Sadeghizadeh M}, title = {Construction of a Sensitive and Specific Lead Biosensor Using a Genetically Engineered Bacterial System With a Luciferase Gene Reporter Controlled by Pbr and Cada Promoters}, journal = {BioMedical Engineering Online}, volume = {19}, number = {1}, pages = {-}, year = {2020} }
RIS	TY - JOUR AU - Nourmohammadi E AU - Hosseinkhani S AU - Nedaeinia R AU - Khoshdelsarkarizi H AU - Nedaeinia M AU - Ranjbar M AU - Ebrahimi N AU - Farjami Z AU - Nourmohammadi M AU - Mahmoudi A AU - Goli M AU - Ferns GA AU - Sadeghizadeh M TI - Construction of a Sensitive and Specific Lead Biosensor Using a Genetically Engineered Bacterial System With a Luciferase Gene Reporter Controlled by Pbr and Cada Promoters JO - BioMedical Engineering Online VL - 19 IS - 1 SP - EP - PY - 2020 ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract