Direct Labelling of Octreotide With 99Mtc: Effect of Different Concentration of Reducing Agents and Amount of Sodium Pertechnetate on Radiolabelling Efficiency

Direct Labelling of Octreotide With 99Mtc: Effect of Different Concentration of Reducing Agents and Amount of Sodium Pertechnetate on Radiolabelling Efficiency Publisher Pubmed

Gandomkar M¹ ; Najafi R¹ ; Sadat Ebrahimi SE² ; Shafiee A² ; Babaei MH¹ ; Rabbani M³ ; Shabani GA¹

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1. Radioisotope Division, Nuclear Research Center, Atomic Energy Organization of Iran, Tehran, Iran
2. Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran Univ. of Medical Sciences, Tehran, Iran
3. Department of Pharmacology, Faculty of Pharmacy, Isfahan Univ. of Medical Sciences, Isfahan, Iran

Source: Applied Radiation and Isotopes Published:2003

Abstract

Octreotide, a synthetic analog of natural hormone somatostatin, was labelled with 99mTc. Labelling was accomplished by reduction of the cysteine bridge, which provided sulfhydryl groups for chelating with 99mTc. Sodium ascorbate and sodium dithionite in different concentrations were used as reducing agents. Different amounts of sodium pertechnetate were used for labelling of peptide. When the mass ratio of peptide and sodium ascorbate was 1:100 and the final concentration of dithionite in the labelling vial was 0.2-0.4μg/μl with 0.18-1.48GBq sodium pertechnetate more than 80% radiolabelling efficiency was confirmed by RP-HPLC, ITLC-SG and C18 Cartridge analysis. The stability of the 99mTc-peptide bond was evaluated by human serum challenge and that showed the stability was 90% after 4h. © 2003 Elsevier Science Ltd. All rights reserved.

Style	Citing Format
MLA	Gandomkar M, et al.. "Direct Labelling of Octreotide With 99Mtc: Effect of Different Concentration of Reducing Agents and Amount of Sodium Pertechnetate on Radiolabelling Efficiency." Applied Radiation and Isotopes, vol. 58, no. 3, 2003, pp. 361-364.
APA	Gandomkar M, Najafi R, Sadat Ebrahimi SE, Shafiee A, Babaei MH, Rabbani M, Shabani GA (2003). Direct Labelling of Octreotide With 99Mtc: Effect of Different Concentration of Reducing Agents and Amount of Sodium Pertechnetate on Radiolabelling Efficiency. Applied Radiation and Isotopes, 58(3), 361-364.
Chicago	Gandomkar M, Najafi R, Sadat Ebrahimi SE, Shafiee A, Babaei MH, Rabbani M, Shabani GA. "Direct Labelling of Octreotide With 99Mtc: Effect of Different Concentration of Reducing Agents and Amount of Sodium Pertechnetate on Radiolabelling Efficiency." Applied Radiation and Isotopes 58, no. 3 (2003): 361-364.
Harvard	Gandomkar M et al. (2003) 'Direct Labelling of Octreotide With 99Mtc: Effect of Different Concentration of Reducing Agents and Amount of Sodium Pertechnetate on Radiolabelling Efficiency', Applied Radiation and Isotopes, 58(3), pp. 361-364.
Vancouver	Gandomkar M, Najafi R, Sadat Ebrahimi SE, Shafiee A, Babaei MH, Rabbani M, et al.. Direct Labelling of Octreotide With 99Mtc: Effect of Different Concentration of Reducing Agents and Amount of Sodium Pertechnetate on Radiolabelling Efficiency. Applied Radiation and Isotopes. 2003;58(3):361-364.
BibTex	@article{ author = {Gandomkar M and Najafi R and Sadat Ebrahimi SE and Shafiee A and Babaei MH and Rabbani M and Shabani GA}, title = {Direct Labelling of Octreotide With 99Mtc: Effect of Different Concentration of Reducing Agents and Amount of Sodium Pertechnetate on Radiolabelling Efficiency}, journal = {Applied Radiation and Isotopes}, volume = {58}, number = {3}, pages = {361-364}, year = {2003} }
RIS	TY - JOUR AU - Gandomkar M AU - Najafi R AU - Sadat Ebrahimi SE AU - Shafiee A AU - Babaei MH AU - Rabbani M AU - Shabani GA TI - Direct Labelling of Octreotide With 99Mtc: Effect of Different Concentration of Reducing Agents and Amount of Sodium Pertechnetate on Radiolabelling Efficiency JO - Applied Radiation and Isotopes VL - 58 IS - 3 SP - 361 EP - 364 PY - 2003 ER -

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