Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share By
A Comparison of Disk Diffusion Method and E-Test in Determining the Susceptibility and Resistance of Klebsiella and Acinetobacter Strains to Cefepime in Patients With Ventilator-Associated Pneumonia Admitted to the Intensive Care Unit Publisher



Mahjobipoor H1 ; Sajadi M2 ; Honarmand A1 ; Rahimivarposht M1 ; Yadegari L3 ; Attarzadeh A4 ; Mahjobipoor L5 ; Fattahpour S6
Authors

Source: Immunopathologia Persa Published:2024


Abstract

Introduction: Ventilator-associated pneumonia (VAP) which is mostly caused by microorganisms such as Acinetobacter and Klebsiella is one of the causes of death among patients in the intensive care unit (ICU). Objectives: The aim of this study was to compare disk diffusion method and E-test in detecting the susceptibility of Klebsiella and Acinetobacter strains isolated from lung secretions of patients with VAP admitted to the ICU treated with cefepime. Patients and Methods: In this cross-sectional descriptive study, 100 patients with VAP including 55 and 45 cases infected with Acinetobacter and Klebsiella, respectively, according to the antibiogram participated. The susceptibility and resistance of Acinetobacter and Klebsiella strains to cefepime was determined using disk diffusion method and E-test. In addition, kappa test was used to determine the sensitivity and specificity of disk diffusion method compared to E-test. Results: The sensitivity and specificity of the disk diffusion method were 100% and 25%, respectively and its positive predictive value was 87.5% and its negative predictive value was 100%. The kappa coefficient was 0.35 (P < 0.0001), which indicates a significant agreement between the two tests. Conclusion: Disk diffusion method has high sensitivity and low specificity in comparison to E-test in determining the resistance and susceptibility of Acinetobacter and Klebsiella strains to cefepime in patients with VAP admitted to the ICU. Copyright © 2024 The Author(s).
Related Docs
View other Related Docs
1. Prevalence Incidence and Clinical Outcome of Klebsiella and Acinetobacter Ventilator-Associated Pneumonia, Immunopathologia Persa (2023)
2. Effect of Nebulized Eucalyptus on Contamination of Microbial Plaque of Endotracheal Tube in Ventilated Patients, Iranian Journal of Nursing and Midwifery Research (2016)
3. Reliability of Disk Diffusion Test Results for the Antimicrobial Susceptibility Testing of Nosocomial Gram-Positive Microorganisms: Is E-Test Method Better?, Iranian Journal of Pharmaceutical Research (2012)
Experts (# of related papers)
View all Related Experts
Hamid Solgi (5)
Hossein Fazeli (4)
Other Related Docs
4. Prevalence and Antibiotic Resistance Pattern of Acinetobacter Species Isolated From Al-Zahra Hospital in Isfahan, Iran, Journal of Isfahan Medical School (2013)
5. Detection of Blactx-M15 and Blaoxa-48 Genes in Gram-Negative Isolates From Neonatal Sepsis in Central of Iran, Iranian Journal of Microbiology (2019)
6. High Rate of Carbapenem-Resistant Klebsiella Pneumoniae Detected From Hospital Equipments in Iran, Acta Microbiologica et Immunologica Hungarica (2018)
7. Bacterial Infections and Outcomes of Inpatients With Covid-19 in the Intensive Care Unit During the Delta-Dominant Phase: The Worst Wave of Pandemic in Iran, Frontiers in Public Health (2024)
8. Frequency Determination of Carbapenem-Resistant Klebsiella Pneumoniae (Crkp) Isolated From Hospitals in Isfahan of Iran and Evaluation of Synergistic Effect of Colistin and Meropenem on Them, Cellular and Molecular Biology (2018)
9. Secondary Bacterial Infection Caused by St16 Ndm-1 and Oxa-48-Producing Colistin and Carbapenem-Resistant Klebsiella Pneumoniae Treated With Tigecycline in a Pregnant Woman With Covid-19, Journal of Pharmaceutical Policy and Practice (2023)
10. Effect of Nebulized Eucalyptus on Ventilator Associated Pneumonia in Ventilated Patients, Journal of Medicinal Plants (2020)
11. Epidemiology, Risk Factors, and Antimicrobial Resistance of Nosocomial Infections in the Intensive Care Unit Trauma Patients: A Cross-Sectional Study, Journal of Research in Medical Sciences (2025)
12. Frequency of Metallo-Β-Lactamase and Antimicrobial Resistance Patterns of Acinetobacter Baumannii in Carbapenem-Resistant Isolates in Intensive Care Units, Journal of Isfahan Medical School (2015)
13. Frequency of Multi-Drug Resistance Acinetobacter Baumannii Isolates in Intensive Care Units (Icu) of Isfahan Hospitals, Iran, Via Molecular Method and Their Antimicrobial Resistance Patterns, Journal of Isfahan Medical School (2014)
14. The Antibiotic Resistance Profiles of Bacterial Strains Isolated From Patients With Hospital-Acquired Bloodstream and Urinary Tract Infections, Critical Care Research and Practice (2012)
15. Characterization of Oxacillinase and Metallo-Β-Lactamas Genes and Molecular Typing of Clinical Isolates of Acinetobacter Baumannii in Ahvaz, South-West of Iran, Jundishapur Journal of Microbiology (2016)
16. Comparative Evaluation of Disk Diffusion and E-Test in Determining the Susceptibility of Helicobacter Pylori to Metronidazole, Clarithromycin and Amoxicillin, Journal of Isfahan Medical School (2013)
17. Prevalence of Metallo-Beta-Lactamase Producing Acinetobacter Baumannii Isolated From Intensive Care Unit in Tertiary Care Hospitals, Annali di Igiene Medicina Preventiva e di Comunita (2018)
18. Outbreak of Colistin and Carbapenem-Resistant Klebsiella Pneumoniae St16 Co-Producing Ndm-1 and Oxa-48 Isolates in an Iranian Hospital, BMC Microbiology (2024)
19. Molecular Characterization of Ndm and Oxa-48-Like-Producing Klebsiella Pneumoniae St16 and Hypervirulent St337 Clone Among Two Patients; a Case Report, BMC Infectious Diseases (2024)
20. Comparison of Inhaled Colistin With Inhaled Amikacin-Fosfomycin in the Treatment of Ventilator-Associated Pneumonia Caused by Extensively Drug-Resistant (Xdr) Acinetobacter: A Clinical Trial, Archives of Clinical Infectious Diseases (2021)